The University of Toledo

Exercise Physiology Lab

 

The Excercise Biology Laboratories

The Exercise Biology Laboratories are on the first floor of the Health Sciences and Human Services Building located on the Main Campus of The University of Toledo.   The Exercise Biology Laboratories consists of two large analytical labs, an Animal Facility, a Cell Culture Lab, and a Microscopy Lab. 

 

 Analytical Labs

The analytical labs contain the following major pieces of equipment that are used to prepare samples and to analysis samples for protein or gene expression:

     Ultra-low temperature freezers
     Tissue homogenizers (e.g., magnet bead homogenizer and cell sonicator) 
     Leica Cryostat
     Molecular Devices UV and fluorescent plate readers
     Gel electrophoresis units
     Wet and semi-dry transfer units
     LI-COR's Odyssey Infrared Imaging System 
     Applied Biosystem’s Real-time PCR System

 

 

 Animal Facility

The animal facility contains a cage washing room, a surgical suite, and housing areas. The surgical room contains computerized equipment for assessing contractile properties of rodent skeletal muscle in situ and in vitro, dissecting microscopes, a down-draft necropsy table, scales, and surgical supplies.  A rodent treadmill is also housed in the facility.

 

 Cell Culture Lab

The Cell Culture Lab contains two biological class II safety cabinets, a Flex Cell Strain Unit, a CO₂ incubator, a refrigerator, a freezer, a cytospin centrifuge, a cell culture grade water purification system, microscopes, an autoclave, and liquid nitrogen storage dewars.

  

 

 Microscopy Lab 

The Microscopy Room houses an Olympus inverted microscope equipped with phase contrast, Nomarski optics, three color fluorescent capabilities, and a computer-interfaced digital camera.