Medical Microbiology and Immunology

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Welcome

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Additional Information

Contact Us

Health Science Campus
Health Education Building
Medical Microbiology and Immunology
Room 229, MS1021
Office: 419-383-4323
Fax: 419-383-3002 Microbiology.Immunology@utoledo.edu

Leah Marie Wuescher, Ph.D.

 

Leah
Research Associate
Affiliation: Randall G. Worth, Ph.D.
College of Medicine and Life Sciences
Department of Medical Microbiology& Immunology
Lab Phone: 419-383-3906
Email:Leah.Wuescher@utoledo.edu

CV

Education                                                                                

The University of Toledo College of Medicine                                              Toledo, OH
Ph.D.                                                                                                       Completed December 2012
Research: Effects of menin on hepatic glucose production


The University of Toledo                                                                                Toledo, OH
B.S.                                                                                                        Completed December 2007
Major: Biology

 

 Publications

Abstracts

  • Wuescher LM, Takashima A, Worth RG. Platelets are Critical for Survival from Staphylococcus aureus Blood Infection in Mice. Midwest Platelet Conference 2014 (Poster presentation)
  • Wuescher LM, Takashima A, Worth RG. Platelets are Critical for Survival from Staphylococcus aureus Blood Infection in Mice. Midwest Microbial Pathogenesis Conference 2013 (Poster presented by RG Worth)
  • Wuescher L, Angevine K, Mensah-Osman EJ. Hepatic FoxO1 Acetylation is Regulated by Menin and Influenced by Insulin Signaling.  Experimental Biology Meeting 2012 (Poster presentation)
  • Wuescher L, Angevine K, Mensah-Osman EJ. Insulin regulates Menin expression, localization and interaction with FOXO 1. 38th Annual Pharmacology Research Colloquium 2011 (Oral presentation)
  • Palladino L, Angevine K, Patel P, Mensah-Osman E, Menin Liver Specific Hemizygous Mice Exhibit a Metabolic Syndrome Phenotype when Challenged with High Fat Diet. 37th Annual Pharmacology Research Colloquium 2010 (Poster presentation)
  • Palladino L, Angevine K, Patel P, Mensah-Osman E, Menin Liver Specific Hemizygous Mice Exhibit a Metabolic Syndrome Phenotype when Challenged with High Fat Diet. First Annual Cardiovascular and Metabolic Diseases Student Research Forum 2010 (Oral presentation, 3rd place award)
  • Palladino L, Burgess A, Vanella L, Kim DH, Sodhi K, Peterson S, Abraham NG, Mensah-Osman E. L-4F Improves Metabolic Syndrome Phenotype in HO-2 Null Mice by Decreasing NFkB Activity & Increasing Adiponectin Levels. Experimental Biology Meeting 2010 (Poster presentation)
  • Palladino L, Angevine K, Kim DH, Sodhi K, Inoue K, Gotlinger KH, Burgess A, Vanella L,  Abraham NG, Mensah-Osman E.  L-4F Rescues the Inflammatory Phenotype of HO-2 null Mice through Induction of HO-1 and Epoxyeicosatrienoic Acids (EETs) and Decreased NF-kB Activity. Winter Eicosanoid Conference 2010 (Oral presentation)
  • Palladino L, Angevine K, Ramakrishnan S, Bowman T, Ledford K, Najjar S, Mensah-Osman E. Menin in the Liver is a Metabolic Sensor Tightly Regulated by Insulin for its Clearance. 36th Annual Pharmacology Research Colloquium 2009 (Poster presentation)
  • Angevine K, Palladino L, Ramakrishnan S, Mensah-Osman E. Menin – A central player in Metabolic and Energy Homeostasis. 36th Annual Pharmacology Research Colloquium 2009

Skills

  • In vivo (mouse): Retro-orbital injection, cardiac stick, intraperitoneal injection, submandibular bleed, subcutaneous injection, tail vein injection, handling/breeding mutant mouse models, ear punch, tail snip, genotyping, retro-orbital bleeding, glucose tolerance testing, insulin tolerance testing, NMR, blood pressure measurement, tissue harvesting, serum analysis, intravital imaging
  •  In vivo (C. elegans): Confocal microscopy, RNA isolation, worm lysis, worm maintenance

 Human procedures: Venipuncture, platelet purification

  • In vitro: T cell isolation, cell culture, lentiviral infection, siRNA and plasmid transfection, western blotting, immunoprecipitation, co-immunoprecipitation, quantitative real time polymerase chain reaction (qPCR), polymerase chain reaction (PCR), agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), flow cytometry, RNA isolation, DNA isolation, ELISA, immunocytochemistry (cell and tissue sections), maxi/mini/midiprep, luciferase, cellular fractionation, bacterial culture, PCR fusion, confocal microscopy

 Research Experience

 University of Toledo College of Medicine                                                   Toledo, OH
Postdoctoral Fellow                                                                                    April 2014-Present
Supervisor: Randall Worth, Ph.D.
The role of platelets in bacterial infection and clearance

University of Toledo                                                                                      Toledo, OH
Postdoctoral Fellow                                                                                   July 2013-April 2014
Supervisor: Richard Komuniecki, Ph.D.

  • Analyzing neuropeptide release in C. elegans ASI neurons

University of Toledo College of Medicine                                                   Toledo, OH
Research Associate                                                                                 January 2013-July 2013
Supervisor: Randall Worth, Ph.D.

  • Analysis of a novel mouse model of chronic platelet depletion

University of Toledo College of Medicine                                                   Toledo, OH
Ph.D. Candidate                                                                                  August 2008-December 2012
Supervisor: Edith Mensah-Osman M.D., Ph.D.

  • Researching the effects of a small peptide on the metabolic syndrome phenotype of a diabetic mouse model

University of Toledo                                                                                      Toledo, OH
Undergraduate Researcher                                                                August 2006-December 2007
Supervisor: Douglas W. Leaman, Ph.D.

  • Characterization of murine XAF1

University of Toledo                                                                                     Toledo, OH
Undergraduate Researcher                                                                               Fall 2007     
Supervisor: Yun-Ming Lin, Ph.D.

  • Developing new catalysts to allow efficient synthesis of biologically interesting small organic molecules

Cuyahoga County Board of Heath -Water Quality Program                    Parma, OH
Water Quality Intern                                                                                 Summers 2005-2007
Supervisor: Harry Stark, RS, MPH

  • Conducted biological and chemical monitoring of streams as a stormwater technician

 Society Memberships

American Society of Pharmacology and Experimental Therapeutics               2009-Present
The American Physiological Society                                                               2011-Present
The American Heart Association                                                                    2013-Present
The International Society for Heart and Lung Transplantation                        2013

 

Academic Honors

3rd Place Award for Oral Presentation                                                            June 2010
First Annual Cardiovascular and Metabolic Diseases Student Research Forum
University of Toledo Tower Prestige Scholarship                                             2004-2007   

Last Updated: 8/3/18