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Flow Cytometry Core Facility
Services
The Flow Core provides high-speed cell analysis for
Immunophenotyping
Cell Tracking and Proliferation DNA Cell Cycle/Tumour Ploidy
DNA Synthesis/Degradation
Chromatin Structure
Gene Expression
Cell Membrane Potential
Surface Charge
Membrane Fusion/Runover
Ion Flux
Redox State
Oxidative Metabolism
Cellular pH Change
Cell Viability/Apoptosis/Necrosis
Intracellular Protein Staining
Total Protein
Enzyme Activity
Lipids
Sulfhydryl Groups/Glutathione
Intracellular Cytokine/Chemokine Expression
ELISA
In addition the Flow Core also offers high-speed cell sorting. This can be used to
isolate/separate cell types (or vesicular bodies), rare populations, as well as specific
clones post-transfection for further cell culture or analysis/experimentation—if a
population can be labeled, distinguished, and gated, it can be sorted.
Samples are prepared by individual investigators who then deliver them to the Core
for flow cytometric analysis or sorting. The core will not prepare cells but can provide
assistance, guidance, and/or generation of publication-quality graphics and experimental
design (Example).
BD Biosciences FACSCalibur (Cell Analysis Only)
Features
More information is available at BD Biosciences website |
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BD FACSAria I High-Speed Cell Sorter
Features
More information is available from BD Biosciences |
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Hours of Operation, Fees, and Scheduling
The Flow Cytometry Core Facility will be staffed from 8:00 a.m. until 4:00 p.m.,
Monday through Friday, and is operated by Sean Linkes, M.S. Any inquiries can be made
by phone at 419.383.3402 or by email.
The Core Facility operates on a Fee-for-Service basis which will include data acquisition,
analysis and consultation if needed.
|
Current Flow Rates |
||
| Assisted | Self-Operation | |
| UT Investigators | $30/hr | $30/hr |
| Outside Users | $30/hr | $30/hr |
Cancellations with less than a 24-hour notice are billed for the scheduled time. Investigators
are not billed for cancellations with at least 24 hour notice. Exceptions will be
determined by the Core Facility.
Scheduling can be done by calling 419.383.3402 or by visiting the facility. No scheduling will be accepted by email.
Located on the second floor of the Health Education Building in Room 253, the lab
is directly accessible from the second floor hallway of the Pharmacy Building and
Dowling Hall.
Parking is located in the front of the Health Education Building in Area 43. Alternate
parking is available in Areas 44, 46 (not pictured above), and 48 with access to Health
Education through the adjoining buildings or basement tunnel.
A full campus map is available for download.
