Medical Microbiology and Immunology

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Health Science Campus
Health Education Building
Medical Microbiology and Immunology
Room 229, MS1021
Office: 419-383-4323
Fax: 419-383-3002 Microbiology.Immunology@utoledo.edu

Akira Takashima, M.D., Ph.D.

 

    

Professor and Chairman

Director, Jacobson Center of Clinical & Translational Research

Director, Center for Biomarker Research & Individualized Medicine (BRIM)

Robert A. Stranahan Endowed Chair

Molecular mechanisms regulating function and behavior of dendritic cells; development of dendritic cell-targeted immunotherapeutics


Telephone: 419.383.5423
Fax:  419.383.3002
E-mail Address: akira.takashima@utoledo.edu 

 
  • Development of dendritic cell (DC)-targeted immunotherapeutic
  • Immunobiology of a newly discovered leukocyte population, termed "neutrophil-DC hybrids"
  • Intravital confocal imaging of dynamic behaviors of DCs, neutrophils and other leukocytes
  • Development and validation of new HTP screening platforms

Dr. Takashima's major research interest is in the biology of DCs, which play crucial roles in the induction of both innate and adaptive immunity. His group has recently identified a new leukocyte population that exhibits the surface phenotypes and functional properties of both neutrophils and DCs.  This population, termed “neutrophil-DC hybrid,” plays dual protective roles against bacterial infection by rapidly capturing and killing bacteria and presenting bacterial antigens to T cells. Interestingly, neutrophil-DC hybrids are derived from immature neutrophils and they emerge in lymphoid and non-lymphoid tissues under inflammatory conditions. Using recently developed, HTP-compatible assay platforms, his group has identified several cytokines and small chemical compounds capable of regulating neutrophil transdifferentiation into hybrid cells. Studies are in progress in his laboratory to define: a) mechanisms regulating generation and function of neutrophil-DC hybrids, b) contributions of hybrids to the pathogenesis of autoimmune inflammatory disease, allergic disorders, and cancer, and c) in vivo impact of the above cytokines and small compounds on the development of neutrophil-DC hybrids.

After receiving his M.D. and Ph.D. from the Nagoya City University Medical School (Nagoya, Japan), he completed his clinical training in Dermatology and post-doctoral research training in Cell Biology. He served on the faculty at the University of Texas Southwestern Medical Center (Dallas, TX) from 1991 until his move to The University of Toledo, when in 2006, he assumed the position of Professor and Chairman of the Department of Medical Microbiology and Immunology and the Robert A. Stranahan Endowed Chair in the College of Medicine. 

Representative Publications (Original Articles):

Matsushima H, Geng S, Lu R, Okamoto T, Yao Y, Mayuzumi N, Kotol PF, Chojnacki BJ, Miyazaki T, Gallo RL, Takashima A. Neutrophil differentiation into a unique hybrid population exhibiting dual phenotype and functionality of neutrophils and dendritic cells. Blood in press 2013

Geng S, Matsushima H, Okamoto T, Yao Y, Lu R,Page K, Blumenthal RM, Ward NL, Miyazaki T, Takashima A. Emergence, origin, and function of neutrophil-dendritic cell hybrids in experimentally induced inflammatory lesions in mice. Blood in press 2013

Fruge RE, Krout C, Lu R, Matsushima H, Takashima, A. Real-time visualization of Macromolecule uptake by epidermal Langerhans cells in living animals. J Invest Dermatol 2012, 132, 609-614.

Miyazawa A, Takashima A. Development and validation of a new in vitro assay designed to measure contact allergen-induced oxidative stress in dendritic cells. J Dermatol Sci 2012, 68, 73-81.

Cho JS, Ramos RI, Hebroni F, Plaisier SB, Xuan C, Granick JL, Matsushima H, Takashima A, Iwakura Y, Cheung AL, Cheng G, Lee DJ, Simon SI, Miller LS. Neutrophil-derived IL-1 beta is sufficient for abscess formation in immunity against Staphylococcus aureus in mice. PLOS Pathogen 2012, 8, e1003047

 Matsushima H, Takashima A  Bidirectional homing of Tregs between the skin and lymph nodes.  J Clin Invest 2010 Mar;120(3):653-656.  doi: 10.1172/JC142280. Epub 2010 Feb 22.

Matsushima H, Ogawa Y, Miyazaki T, Tanaka H, Nishibu A, Takashima A  Intravital imaging of IL-1beta production in skin.  J Invest Dermatol2010;130:1571-1580. 

Mayuzumi N, Matsushima H, Takashima A IL-33 promotes DC development in BM culture by triggering GM-CSF production. Eur J Immunol 2009;39:3331-3342.   

Tanaka H*, Matsushima H, Mizumoto N, Clausen B, Takashima A Classification of chemotherapeutic agents based on their differential in vitro impacts on dendritic cells. Cancer Res 2009;69:6978-6986.  *These authors contributed equally to this work.

Tanaka H*, Matsushima H, Nishibu A, Takashima A. Dual therapeutic efficacy of vinblastine as a unique chemotherapeutic agent capable of  inducing dendritic cell maturation. Cancer Res 2009;69:6987-6994.  *These authors contributed equally to this work.

Matsushima H, Tanaka H, Mizumoto N, Takashima A.  Identification of crassin acetate as a new immunosuppressant triggering heme oxygenase-1 expression in dendritic cells.  Blood 2009 Jul 2;114:64-73.   

Mizumoto N, Tanaka H, Matsushima H, Vishwanath M, and Takashima A  Colchicine promotes antigen cross-presentation by murine dendritic cells.  J. Invest Dermatol 2007;127:1543-1546.

Takashima, A, Liu, FT.  Dermal immunopathology:  from genetics to effector mechanisms. Clin Rev Allergy Immunol 2007;33:1-3.

Mohr, R, Takashima A.  Epidermal langerhands movement in situ: a model for understanding immunologic function in the skin. Arch Dermatol  2007;143:1352.

Mizumoto N, Tanaka H, Matsushima H, Vishwanath M, Takashima A.  Colchicine promotes antigen cross-presentation by murine dendritic cells. J Invest Dermatol 127:1543-1546, 2007.

Nishibu A, Ward BR, Boes M, Takashima A. Roles for IL-1 and TNFalpha in dynamic behavioral responses of Langerhans cells to topical hapten application. J Dermatol Sci 45:23-30, 2007.

Ward BR, Jester JV, Nishibu A, Vishwanath M, Shalhevet D, Kumamoto T, Petroll WM, Cavanagh HD, Takashima A. Local thermal injury elicits immediate dynamic behavioural responses by corneal Langerhans cells. Immunology 120:556-572, 2007.

Gao J, Huang S, Li M, Luo R, Wang X, Takashima A.  GM-CSF-surface-modified B16.F10 melanoma cell vaccine.  Vaccine Jun 19;24(25):5265-8, 2006.

Nishibu A, Ward BR, Jester JV, Ploegh HL, Boes M, Takashima A.  Behavioral responses of epidermal Langerhans cell in situ to local pathological stimuli. J Invest Dermatol 126:787-796, 2006.

Ogawa Y, Mizumoto N, Tanaka H, Matsushima H, Takashima A.  Identification of Novel Pharmacological Activities of an Antifungal Agent, Nystatin, to Promote Dendritic Cell Maturation.  J Invest Dermatol 126:349-353, 2006.

Tohyama M, Dai X, Sayama K, Yamasaki K, Shirakata Y, Hanakawa Y, Tokumaru S, Yahata Y, Yang L, Nagai H, Takashima A, Hashimoto K.  dRNA-mediated innate immunity of epidermal keratinocytes. Biochemical and Biophysical Research Communications 335:505-511, 2005.

Lu M, Zhang M, Takashima A, Weiss J, Apicella MA, Li X-H, Yuan D, Munford RS. LPS deacylation by endogenous lipase controls innate antibody responses to Gram-negative bacteria. Nature Immunol 6:989-994, 2005.

Mizumoto N, Gao J, Matsushima H, Ogawa Y, Tanaka H, Takashima A. Discovery of novel immunostimulants by dendritic cell-based functional screening. Blood 106:3082-2089, 2005.

Mizumoto N, Hui F, Edelbaum D, Weil MR, Wren JD, Shalhevet D, Matsue H, Liu L, Garner HR, Takashima A. Differential activation profiles of multiple transcription factors during dendritic cell maturation. J Invest Dermatol 124:718-724, 2005.

Huebeschman ML, Hunt J, Munjuluri B, Takashima A, Garner HR. Design and performance of variable spectrum synthesizer. Rev Sci Instruments 75:4845-4855, 2004.

Matsue H, Matsue K, Edelbaum D, Walters M, Morita A, Takashima A.  New strategy for efficient selection of dendritic cell-tumor hybrids and clonal heterogeneity of resulting hybrids.  Cancer Biol & Therapy 3:1145-1151, 2004.

McGuire M, Sykes K, Samli K, Timares L, Barry M, Stemke-Hale K, Tagliaferri F, Logan M, Jansa K, Takashima A, Brown K, Johnston SA. A library-selected, Langerhans cell-targeting peptide enhances an immune response.  DNA & Cell Biology 23:742-752, 2004.

Gao J, Takashima A. Cloning and characterization of Trichophyton ruburum genes encoding actin, Tri r2, and Tri r4. J Clin Microbiol 42:3298-3299, 2004.

Mummert DI, Takashima A, Mummert ME. Langerhans cells in CD44-deficient mice emigrate from the epidermis but fail to reach the lymph nodes after hapten application.  J Invest Dermatol 122:846-847, 2004.

Matsue H, Edelbaum, Shalhevet D, Mizumoto N, Yang C, Mummert ME, Oeda J, Masayasu H, Takashima A. Generation and function of reactive oxygen species in dendritic cells during Ag presentation.  J Immunol 171:3010-3018, 2003.

Mummert DI, Takashima A, Ellinger L, Mummert MA. Involvement of hyaluronan in epidermal Langerhands cell maturation and migration in vivo. J Dermatol Sci 33:91-97, 2003.

Mizumoto N, Mummert ME, Shalhevet D, Takashima A. Keratinocyte ATP release assay for testing skin irritating potentials of structurally diverse chemicals.  J. Inves Dermatol 121: 1066-1072, 2003.

Timares L, Safer KM, Qu B, Takashima A, Johnston SA. Drug-inducible dendritic cell-based  genetic immunization. J Immunol 170:5483-5490, 2003.

Kumamoto T, Shalhevet D, Matsue H, Mummert ME, Ward BR, Jester JV, Takashima A. Hair follicles serve as local reservoirs of skin mast cell precursors. Blood 103:1654-1660, 2003.

LuM, Zhang M, Kitchens RL, Fosmire S, Takashima A, Munford RS. Stimulus-dependent eacylation of bacterial lipopolysaccharide by dendritic cells. J Exp Med 197:1745-1754, 2003.

Mummert ME, Mummert DI, Ellinger L, Takashima A. Functional roles of hyaluronan in B16-10 melanoma growth and experimental metastasis in mice. Mol Cancer Therapeutics 2:295-300, 2003.

Wolfe T, Asseman C, Hughes A, Matsue H, Takashima A, von Herrath MG. Reduction of anti-viral CD8 lymphoycytes in vivo with dendritic cells expressing FAS ligand – indreased survival of viral (lymphocytic chorimeningitis virus) central nervous system infection. J Immunol 169:4867-4782, 2002.

Matsue H, Yang C, Matsue K, Edelbaum D, Mummert, M, Takashima A. Contrasting impacts of immunosuppressive agents (rapamycin, FK506, cyclosporin A, and dexamethasone) on bi-directional dendritic cell-T cell interaction during antigen presentation. J Immunol 169:3555-3564, 2002.

Mummert M, Mummert D, Edelbaum D, Hui F, Matsue H, Takashima A. Synthesis and surface expression of hyaluronan by dendritic cells and its potential role in antigen presentations. J Immunol 169:4322-4331, 2002

Kusuhara M, Matsue K, Edelbaum D, Loftus J, Takashima A, Matsue H. Killing of naVve T cells by CD95L-transfected dendritic cells (DC): In vivo study using killer DC-DC hybrids and CD4+ T cells from DO11.10 mice. Eur J Immunol 32:1035-1043, 2002.

Mizumoto N, Kumamoto T, Robson SC, Sevigny J, Matsue H, Enjyoji K, Takashima A.  CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness. Nature Med 8:358-365, 2002.

Kumamoto T, Huang EK, Paek HJ, Morita A, Matsue H, Valentini RF, Takashima A. Induction of tumor-specific protective immunity by in situ Langerhans cell vaccine Nature Biotech 20:64-69, 2002.

Yokota K, Takashima A, Bergstresser PR, Ariizumi K. Identification of a human homologue of the dendritic cell-associated C-type lectin-1, dectin 1. Gene 272:51-60, 2001.

Matsue H, Matsue K, Kusuhara M, Kumamoto T, Okumura K, Yagita H, Takashima A. Immunosuppressive properties of CD95L-transduced "killer" hybrids created by fusing donor- and recipient-derived dendritic cells. Blood 98:3465-3472, 2001.

Morita A, Ariizumi K, Ritter R, Jester JV, Kumamoto T, Johnson SA, Takashima A.  Development of Langerhans cells-targeted gene expression system using dectin-2 promoter.  Gene Therapy 8:;1729-1737, 2001.

Bouis DA, Popova TG, Takashima A, Norgard MV. Dendritic cells phagocytose and are activated by treponema pallidum. Infect Immunol 69:518-528, 2001.

Fehr BS, Takashima A, Bergstresser PR, Cruz PD Jr.  T cells reactive to keratinocyte antigens are generated during induction of contact hypersensitivity in mice. A model for autoeczematization in humans? Am J Contact Derm 11:145-154, 2000.

Mummert ME, Mohamadzadeh M, Mummert DI, Mizumoto N, Takashima A.  Development of a peptide inhibitor of hyaluronan-mediated leukocyte trafficking. J Exp Med 192:769-779, 2000.

Abeyama K, Eng W, Jester JV, Edelbaum D, Cockerell CJ, Bergstresser PR, Takashima A.  A role for NFkB gene transactivation in sunburn. J Clin Invest 105:1751-1759, 2000.

Hayashi S, Johnston SA, Takashima A.  Induction of Th2-directed immune responses by IL-4-transduced dendritic cells in mice. Vaccine 18:3097-3105, 2000.

Ariizumi K, Shen  G-L, Shikano S, Ritter III R, Zukas P, Edelbaum D, Morita A, Takashima A:  Cloning of a second dendritic cell-associated c-type lectin (dectin-2) and its alternatively spliced isoforms. J Biol Chem 275:11957-11963, 2000.

Ariizumi K, Shen  G-L, Shikano S, Xu S, Ritter III R, Kumamoto T,  Edelbaum D, Morita A, Bergstresser PR, Takashima A. Identification of a novel, dendritic cell-associated molecule, dectin-1, by subtractive cDNA cloning. J Biol Chem 275: 20157-20167, 2000.

Matsue H, Matsue K, Walters M, Okumura K, Yagita H, and Takashima A. Induction of antigen-specific immunosuppression by CD95L cDNA-transfected "killer" dendritic cells. Nature Med 5:930-937,  1999. 

Matsue H, Edelbaum D, Hartmann AC, Morita A, Bergstresser PR, Yagita H, Okumura K, and  Takashima A. Dendritic cells undergo rapid apoptosis in vitro during antigen-specific interaction with CD4+ T cells.  J Immunol  162:5287-5298, 1999.

Hosoi J, Tsuchiya T, Denda M, Ashida Y, Takashima A, Granstein RD, Koyama J.  Modification of LC phenotype and suppression of contact hypersensitivity response by stress. J Cutan Med Surg 3:79-84, 1998. 

Timares L, Takashima A, Johnston SA.  Quantitative analysis of the immunopotency of genetically transfected dendritic cells. Proc Natl Acad Sci USA  95:13147-13152, 1998.

Aragane Y, Schwarz A, Luger TA, Ariizumi K, Takashima A, Schwarz T.  Ultraviolet light suppresses IFNγ-induced IL-7 gene transcription in murine keratinocytes by interfering with interferon regulatory factors.  J Immunol 158:5393-5399, 1997.

Torii H, Hosoi J, Beissert S, Xu S, Fox FE, Asahina A, Takashima A, Rook AH, Granstein RD.  Regulation of cytokine expression in macrophages and the Langerhans cell-like line XS52 by calicitonin gene-related peptide.  J Leukocyte Biol 61:216-223, 1997.

Ogoshi M, Takashima A, Taylor RS.  Mechanisms regulating telomerase activity in murine T cells.  J Immunol 158:622-628, 1997.

Catalina MD, Carroll MC, Arizpe H, Takashima A, Estess P, Siegelman MH.  The route of antigen entry determines the requirement for L-selectin during immune responses.  J Exp Med 184:2341-2351, 1996.

Kitajima T. Ariizumi K, Bergstresser PR, Takashima A.  UVB radiation sensitizes an epidermal dendritic cell line (XS52) to undergo apoptosis in response to antigen-specific interaction with T cells.  J Immunol 157:3312-3316, 1996.

Kitajima T, Caceres-Dittmar G, Tapia FJ, Jester J, Bergstresser PR, Takashima A.  T cell-mediated terminal maturation of dendritic cells: Loss of adhesive and phagocytotic capacities.  J Immunol 157:2340-2347, 1996.

Yokota K, Ariizumi K, Kitajima T, Bergstresser PR, Street NE, Takashima A.  Cytokine-mediated communication between dendritic epidermal T cells and Langerhans cells: In vitro studies using cell lines.  J Immunol 157:1529-1537, 1996.

Kitajima T, Ariizumi K, Bergstresser PR, Takashima A.  A novel mechanism of glucocorticoid-induced immune suppression: The inhibition of T cell-mediated terminal maturation of a murine dendritic cell line.  J Clin Invest 98:142-147, 1996.

Qureshi AA, Hosoi J, Xu S, Takashima A, Granstein R, Lerner EA.  Langerhans cells express inducible nitric oxide synthase and produce nitric oxide.  J Invest Dermatol 107:815-821, 1996.

Mohamadzadeh M, McGuire MJ, Smith DJ, Gaspari AA, Bergstresser PR, Takashima A.  Functional roles for granzymes in murine epidermal γδ T cell-mediated killing of tumor targets. J Invest Dermatol 107:738-742, 1996.

Mohamadzadeh M, Poltorak AN, Bergstresser PR, Beutler B, Takashima A.  Dendritic cells produce macrophage inflammatory protein-1γ, a new member of the CC chemokine family.  J Immunol 156:3102-3106, 1996.

Schuhmachers G, Ariizumi K, Kitajima T, Edelbaum D, Xu S, Shadduck RK, Gilmore GL, Taylor RS, Bergstresser PR, Takashima A.  UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism. J Invest Dermatol 106:1023-1029, 1996.

Ariizumi K, Bergstresser P, Takashima A.  Wavelength-specific induction of immediate early genes by ultraviolet radiation.  J Dermatol Sci 12:147-155, 1996.

Ono M, Ariizumi K, Bergstresser PR, Takashima A.  IL-7 upregulates T cell receptor/CD3 expression by cultured dendritic epidermal T cells.  J Dermatol Sci 11:89-96, 1996.

Mohamadzadeh M, Ariizumi K, Sugamura K, Bergstresser PR, Takashima A.  Expression of the common cytokine receptor γ chain by murine dendritic cells including epidermal Langerhans cells.  Eur J Immunol 26:156-160, 1996.

Mohamadzadeh M, Takashima A, Dougherty I, Knop J, Bergstresser PR, Cruz PD Jr.  Ultraviolet B radiation up-regulates the expression of IL-15 in human skin.  J Immunol 155:4492-4496, 1995.

Kitajima T, Ariizumi K, Mohamadzadeh M, Edelbaum D, Bergstresser PR, Takashima A.  T cell-dependent secretion of IL-1β by a dendritic cell line (XS52) derived from murine epidermis.  J Immunol 155:3794-3800, 1995.

Kitajima T, Ariizumi K, Bergstresser PR, Takashima A.  T cell-dependent loss of proliferative responsiveness to CSF-1 by a murine epidermal-derived dendritic cell line, XS52.  J Immunol 155:5190-5197, 1995.

XuS, Bergstresser PR, Takashima A. Phenotypic and functional heterogeneity among murine epidermal-derived dendritic cell clones.  J Invest Dermatol 105:831-836, 1995.

Edelbaum D, Mohamadzadeh M, Bergstresser PR, Sugamura K, Takashima A.  Interleukin (IL)-15 promotes the growth of murine epidermal γδ T cells by a mechanism involving the β- and γc-chains of the IL-2 receptor.  J Invest Dermatol 105:837-843, 1995.

Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A.  2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors.  J Invest Dermatol 105:592-596, 1995.

Ariizumi K, Kitajima T, Bergstresser PR, Takashima A.  Interleukin-1β converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines.  Eur J Immunol 25:2137-2140, 1995.

Schuhmachers G, Xu S, Bergstresser PR, Takashima A.  Identity and functional properties of novel skin-derived fibroblast lines (NS series) that support the growth of epidermal-derived dendritic cell lines.  J Invest Dermatol 105:225-230, 1995.

Asahina A, Moro O, Hosoi J, Lerner EA, Xu S, Takashima A, Granstein RD.  Specific induction of cAMP in Langerhans cells by calcitonin gene-related peptide: Relevance to functional effects.  Proc Natl Acad Sci USA 92:8323-8327, 1995.

XuS, Ariizumi K, Edelbaum D, Bergstresser PR, Takashima A.  Cytokine-dependent regulation of growth and maturation in murine epidermal dendritic cell lines.  Eur J Immunol 25:1018-1024, 1995.

Takashima A, Edelbaum D, Kitajima T, Shadduck RK, Gilmore G, Xu S, Taylor RS, Bergstresser PR, Ariizumi K. Colony-stimulating factor-1 secreted by fibroblasts promotes the growth of dendritic cell lines (XS series) derived from murine epidermis.  J Immunol 154:5128-5135, 1995.
 
Schuhmachers G, Ariizumi K, Mathew PA, Bennett M, Kumar V, Takashima A.  Activation of murine epidermal γδ T cells through surface 2B4.  Eur J Immunol 25:1117-1120, 1995.

Caceres-Dittmar G, Ariizumi K, Xu S, Tapia FJ, Bergstresser PR, Takashima A.  Hydrogen peroxide mediates UV-induced impairment of antigen presentation in a murine epidermal-derived dendritic cell line.  Photochem Photobiol 62:176-183, 1995.

Ariizumi K, Meng Y, Bergstresser PR, Takashima A.  IFN-γ-dependent IL-7 gene regulation in keratinocytes.  J Immunol 154:6931-6039, 1995.

Pandya AG, Sontheimer RD, Cockerell CJ, Takashima A, Piepkorn M.  Papulonodular mucinosis associated with systemic lupus erythematosus: Possible mechanisms of increased glycosaminoglycan accumulation.  J Amer Acad Dermatol 32:199-205, 1995.

XuS, Ariizumi K, Caceres-Dittmar G, Edelbaum D, Hashimoto K, Bergstresser PR, Takashima A.  Successive generation of antigen-presenting, dendritic cell lines from murine epidermis.  J Immunol 154:2697-2705, 1995.

Lyson K, Ceriani G, Takashima A, Catania A, Lipton JM.   Binding of anti-inflammatory α-melanocyte-stimulating-hormone peptides and proinflammatory cytokines to receptors on melanoma cells.  Neuroimmodulation 1:121-126, 1994.

Fehr B, Takashima A, Matsue H, Gerometta J, Bergstresser P, Cruz, P.D., Jr.  Contact sensitization induces proliferation of heterogeneous populations of hapten-specific T cells.  Exp Dermatol 3:189-197, 1994.

Matsue H, Bergstresser PR, Takashima A.   Keratinocyte-derived IL-7 serves as a growth factor for dendritic epidermal T cells in mouse skin.  J Immunol 151:6012-6019, 1993. 

Mathew PA, Garni-Wagner BA, Land K, Takashima A, Stoneman E, Bennet M, Kumar V.  Cloning and characterization of a molecule associated with non-MHC restricted killing mediated by natural killer cells and T cells.  J Immunol 151:5328-5337, 1993.

Kaminski MJ, Cruz PD Jr., Bergstresser PR, Takashima A.  Killing of skin-derived tumor cells by mouse dendritic epidermal T cells.  Cancer Res 53:4014-4019, 1993.

Chung BS, Bergstresser PR, Takashima A.  Mouse dendritic epidermal T cells exhibit chemotactic migration toward Pam 212 keratinocyte culture supernatants. J Invest Dermatol101:371-376, 1993.

Matsue H, Bergstresser PR, Takashima A.  Reciprocal cytokine-mediated cellular interactions in mouse epidermis: promotion of γδ T cell growth by IL-7 and TNFα and inhibition of keratinocyte growth by γIFN.  J Invest Dermatol 101:543-548, 1993.

Matsue H, Cruz PD Jr., Bergstresser PR, Takashima A.  Profiles of cytokine mRNA expressed by dendritic epidermal T cells in mice. J Invest Dermatol 101:537-542, 1993.

Kaminski MJ, Bergstresser PR, Takashima A.  In vivo activation of mouse dendritic epidermal T cells in sites of contact dermatitis.  Eur J Immunol 23:1715-1718, 1993.

Kanazaki T, Morita A, Takashima A.  Follicular keratosis of the chin.  J Amer Acad Dermatol 26:134-135, 1992.

Matsue H, Cruz PD Jr., Bergstresser PR, Takashima A. Langerhans cells are the major source of RNA for IL-1ß and MIP-1α among unstimulated mouse epidermal cells.  J Invest Dermatol 99:537-431, 1992.

Matsue H, Rothberg KG, Takashima A, Kamen BA, Anderson RGW, Lacey SW.   Folate receptor allows cells to grow in low concentrations of 5-methyltetrahydrofolate.  Proc Natl Acad Sci USA 89:6006-6009, 1992.

Takashima A, Yasuda S, Mizuno N.  Determination of the action spectrum for UV-induced plasminogen activator synthesis in mouse keratinocytes in vitro.  J Dermatol Sci 4:11-17, 1992.

Takashima A, Yamamoto K, Kimura S, Takakuwa Y, Mizuno N.  Allergic contact and photocontact dermatitis due to psoralens in patients with psoriasis treated with topical PUVA.  Br J Dermatol 124:37-42, 1991.

Takashima A, Morita A.  Genomic, phenotypic and functional analyses of T cells in patients with psoriasis undergoing systemic cyclosporin A treatment.  J Invest Dermatol 96:376-382, 1991.

Takashima A, Nixon-Fulton JL, Bergstresser PR, Tigelaar RE.   Thy-1+ dendritic epidermal cells in mice: Precursor frequency analysis and cloning of Concanavalin A-reactive cells.  J Invest Dermatol 90:671-678, 1988.

Kuziel WA, Takashima A, Bonyhadi M, Bergstresser PR, Allison JP, Tigelaar RE, Tucker PW.   Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells.  Nature 328:263-266, 1987.

Grinnell F, Takashima A, Lamke-Seymour.  Differentiation of keratinocytes on fibroblast reorganized collagen gels.  Cell Tissue Res 246:13-21, 1986.

Takashima A, Billingham RE, Grinnell F.  Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing.  J Invest Dermatol 86:585-590, 1986.

Takashima A, Grinnell F.  Fibronectin-mediated keratinocyte migration and initiation of fibronectin receptor function in vitro.  J Invest Dermatol 85:304-308, 1985.

Takashima A, Grinnell F.  Human keratinocyte adhesion and phagocytosis prompted by fibronectin.  J Invest Dermatol 83:352-358, 1984.

Representative Publications (Review Articles and Chapters):

Wilgus M-L, Adcock PA, Takashima A. Volume, trend, and citation analyses of skin related publications from 1966 to 2003. J Dermatol Sci 37:125-136 2005.

Mizumoto N, Takashima A.  CD1a and langerin: acting as more than Langerhans cell markers.  J Clin Invest,  113:658-660, 2004.

Takashima A, Matsue H, Kumamoto T, Mizumoto N, Morita A, Mummert M.  Dendritic cells as a target for therapeutic intervention of contact hypersensitivity, in Immune Mechanisms in Allergic Contact Dermatitis. Eds. Cavani, A, Landes Bioscience, Georgetown, TX.  128-135, 2004.

Matsue H, Kusuhara M, Matsue K, Takashima A.  Dendritic Cell-Based Immunoregulatory Strategies.  Intl Arch Allergy Immunol 127:251-258, 2002.

Takashima A, Matsue H.  Antigen presentation and biology of dendritic cells, in Immune Mechanics in Cutaneous Disease, Second Edition. Eds.Kupper TS, Norris DA: Marcel Dekker, Inc., New York, 2002.
 
 Kumamoto T, Morita A, Takashima A.  Recent advances in dendritic cell vaccines for cancer treatment. 28:658-662, J Dermatol 2001.

Takashima A, Matsue H.  Development and testing of dendritic cell lines, in Dendritic Cells: Biology and Clinical Applications, 2nd Edition. Eds. Lotze MT, Thomson AW: Academic Press Ltd., London, UK. 165-178, 2001.

Takashima A, Bergstresser PR.  Derivation of dendritic cell lines from mouse skin, in Dendritic Cell Protocols. Eds. Robinson S, Stagg A., Humana Press, Jew Jersey. 243-254, 2001.

Takashima A, Mummert ME, Kitajima T, Matsue H.  New technologies to prevent and treat contact hypersensitivity responses. Ann NY Acad Sci 919:205-213, 2000.

Matsue M, Morita A, Matsue K, Takashima A.  New technologies toward dendritic cell-based cancer immunotherapies. J Dermatol 26:757-763. 1999.

Takashima A, Morita A.  Dendritic cells in genetic immunization. J Leuko Biology 66:350-356, 1999.

Takashima A, Bergstresser PR.  Impact of UVB radiation on Langerhans cells: in vitro studies using XS 52 cell line, in Biologic Effects of Light 1998. Eds. M.F. Holick & EG Jung, Kluwer Academic Publishers, Boston. 181-190.

Morita A, Takashima A.  Roles of Langerhans cells in genetic immunization.  J Dermatol Sci  20:39-52 1999.

Matsue H, Takashima A. Apoptosis in dendritic cell biology. J Dermatol Sci 20:159-171, 1999.

Kitajima T, Takashima A.  Langerhans cell responses to ultraviolet B radiation. J Dermatol Sci 19:153-160, 1999.

Takashima A.  Preparation and Insolation of Cells: Preparing Fibroblasts, in Current Protocols in Cell Biology, Eds. Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwarts J, Yamada KM,  John Wiley & Sons, Inc., New York, 1999.

Takashima A, Kitajima T.  T cell mediated terminal maturation of dendritic cells, a critical transition into fully potent antigen presenting cells. Pathologie Biologie 46:53-60, 1998.

Bergstresser PR, Kitajima T, Xu S, Ariizumi K, Takashima A. T cell-mediated terminal maturation of dendritic cells, in Dendritic Cells in Fundamental and Clinical Immunology, Ed. Ricchiardi-Castagnoli P, Plenum Publishing, New York.  p. 449-454, 1997.

Ariizumi K, Bergstresser PR, Takashima A.  Subtractive cDNA cloning: A new approach to understanding dendritic cell biology, in Dendritic Cells in Fundamental and Clinical Immunology, Ed. Ricchiardi-Castagnoli P, Plenum Publishing Company Ltd., London, 1997.

Bergstresser PR, Elmets CA, Takashima A, Mukhtar H.  Photocarcinogenesis. Photodermatology, Photoimmunology & Photomedicine 11:181-184, 1996.

Takashima A, Bergstresser PR.  Cytokine-mediated communication by keratinocytes and Langerhans cells with dendritic epidermal T cells.  Seminars in Immunology 8:333-339, 1996.

Takashima A and Bergstresser PR. Impact of UVB Radiation on the Epidermal Cytokine Network.  Photochem Photobiol 63:397-400, 1996.

Takashima A, Matsue H, Bergstresser PR, Ariizumi K.  Interleukin-7-dependent interaction of dendritic epidermal T cells with keratinocytes.  J Invest Dermatol 105:50S-53S, 1995.

Bergstresser PR, Takashima A.  Ultraviolet Radiation-Mediated Defects in Langerhans Cell Function, in The Immune Functions of Epidermal Langerhans Cells, Ed. Heidrun Moll, R.G. Landes Company, Springer-Verlag, London, pp 119-140, 1995.

Takashima A.  UVB-dependent modulation of epidermal cytokine network: Roles in UVB-induced depletion of Langerhans cells and dendritic epidermal T cells.  J Dermatol 22:876-887, 1995.

Takashima A, Xu S, Ariizumi K, Bergstresser PR. Establishment and characterization of antigen-presenting cell lines (XS series) derived from newborn mouse epidermis.  In Dendritic Cells in Fundamental and Clinical Immunology, Eds. J Banchereau and D Schmitt, Plenum Publishing Company Ltd., London, pp 159-162, 1995.

Last Updated: 8/27/15