Equipment and Facility
The Electron Microscopy facility operates two transmission electron microscopes, FEI Tecnai G2 Spirit and Phillips CM10 PW6020 TEM.
The FEI Tecnai G2 Spirit TEM serves solely for clinical diagnostic purposes and is equipped with the Olympus Soft Imaging system and the iTEM software program. The Tecnai resolves ultrastructures up to 0.2 nm, with accelerating voltage that ranges 20-100kV and magnifies from 18k to 650k times.
The Phillips CM10 PW6020 is available for use by researchers independently with prior approval from the facility director (W. Gunning, PhD) and training from one of the EM laboratory technologists. The TEM has an accelerating voltage range of 40-100kV and magnification from 18k to 450k times. Its camera chamber can hold up to 36 sheets of negative film.
The EM laboratory has the following instrumentation for clinical and research work.
Leica EM UC6 Ultramicrotome
Leica Reichert Ultracut S
LKB Bromma Nova Ultratome
Denton Vacuum Evaporator
LKB Bromma 7800 Knifemaker
Olympus BH-2 Light Microscope equipped with Evolution MP Color Media Cybernetics Imaging System
Reichert-Jung 820 Microtome Cambridge Instruments GmbH
OMU3 Ultramicrotome C. Reichert, Austria
MT6000-XL Ultramicrotome RMC, Inc.
LKB Bromma Ultramicrotome
Dent-X 8 CR Negative Film Development Equipment
Beem Drying Cabinet
Kodamatic 42A Processor
Reichert HistoSTAT Rotary Microtome 820
Leitz 1512 Microtome
Laboratory Microwave LMV-1-120 TBS, Inc.
Leica EG1160 Paraffin Block Maker