The University of Toledo

Publications

• D. S. Tawfik and R. E. Viola, Arsenate replacing phosphate – alternative life chemistries and ion promiscuity, Biochemistry 50, 1128-1134 (2011).
• R. E. Viola, C. R. Faehnle, J. Blanco, R. A. Moore, X. Liu, B. T. Arachea and A. G. Pavlovsky, The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis, J. Amino Acids, vol. 2011, Article ID 352538, 11 pages, 2011. (invited review).
• S. Zano, R. Malik, S. Szucs, R. Matalon, and R. E. Viola, Modification of Aspartoacylase for Potential Use in Enzyme Replacement Therapy for the Treatment of Canavan Disease, Molec. Genet. Metabol., 102, 176-180 (2011).
• G. Gao, X. Liu, A. G. Pavlovsky, and R. E. Viola, Identification of Selective Enzyme Inhibitors by Fragment-library Screening, J. Biomolec. Screen., 15, 1042-1050 (2010).
• R. Malik and R. E. Viola, Tartrate Dehydrogenase: a versatile enzyme catalyzing multiple reactions, Acta Cryst. D66, 673-684 (2010).
• B. T. Arachea, X. Liu, A. G. Pavlovsky and R. E. Viola, Expansion of the Aspartate β-semialdehyde Dehydrogenase Family. The First Structure of a Fungal Ortholog, Acta. Cryst. D66, 205-212 (2010).
• D. V. Camper and R. E. Viola, Fully Automated Protein Purification, Anal. Biochem. 393, 176-181 (2009).
• S. W. Bearden, C. Sexton, J. Pare, J. M. Fowler, C. G. Arvidson, L. Yerman, R. E. Viola, and R. R. Brubaker, Attenuated Enzootic (Pestoides) Isolates of Yersinia pestis express Active Aspartase, Microbiology 155, 198-209 (2009).
• X. Liu, A. Pavlovsky, and R. E. Viola, The Structural Basis for Allosteric Inhibition of a Threonine-sensitive Aspartokinase, J. Biol. Chem. 283, 16216-16225 (2008).
• R. E. Viola, L. Yerman, J. M. Fowler, C. G. Arvidson and R. R. Brubaker, A Missense Mutation Causes Aspartase deficiency in Yersinia pestis, Microbiology 154, 1271-1280 (2008).
• J. Le Coq, A. Pavlovsky, R. Malik, R. Sanishvili, C. Xu, and R. E. Viola, Examination of the Mechanism of Human Brain Aspartoacylase through the Binding of an Intermediate Analog, Biochemistry 47, 3484-3492 (2008).
• R. E. Viola, X. Liu, J. F. Ohren, and C. R. Faehnle, Structure of a redundant enzyme. The second isoform of Aspartate β-semialdehyde dehydrogenase from Vibrio cholerae, Acta. Cryst. D64, 321-330 (2008).
• X. Liu, L. Hanson, P. Langan, and R. E. Viola, The Effect of Deuteration on Protein Structure. A High Resolution Comparison of Hydrogenous and Perdeuterated Haloalkane Dehalogenase, Acta Cryst D63, 1000-1008 (2007)
• D. M. Coe and R. E. Viola, Assessing the Roles of Catalytic Functional Groups in the Mechanism of Homoserine Succinyltransferase, Arch. Biochem. Biophys. 461, 211-218 (2007).
• J. F. Ohren, M. L. Kundracik, C. L. Borders, P. Edmiston, and R. E. Viola, Subunit Asymmetry and Communication in Muscle Creatine Kinase, Acta Cryst. D63, 381-389 (2007).
• R. E. Viola, The Impact of Structural Biology on Neurobiology, Proc. Natl. Acad. Sci. USA 104, 399-400 (2007).
• C. R. Faehnle, X. Liu, A. Pavlovsky, and R. E. Viola, The Initial Step in the Archael Aspartate Biosynthetic Pathway Catalyzed by a Monofunctional Aspartokinase, Acta Cryst. F62 , 962-966 (2006).
• C. R. Faehnle, J. Le Coq, X. Liu, and R. E. Viola, Structural Complexes of the Anti-bacterial Target Aspartate-β-semialdehyde Dehydrogenase from Streptococcus pneumoniae, J. Biol. Chem. 281, 31031-31040 (2006).
• J. Le Coq, H.-J. An, C. Lebrilla, and R. E. Viola, Characterization of Aspartoacylase: the Brain Enzyme Responsible for Canavan Disease, Biochemistry 45, 5878-5884 (2006).
• Q. Liu, J. R. Kirchhoff, C. R. Faehnle, R. E. Viola, and R. A. Hudson, A Rapid Method for the Purification of Methanol Dehydrogenase from Methylobacterium extorquens, Prot. Express. Purif. 46, 316-320 (2006).
• D. M. Colleluori, R. S. Reczknwski, F. A. Emig, E. Cama, J. D. Cox, L. R. Scolnick, K. Compher, K. Jude, S. Han, R. E. Viola, D. W. Christianson, and D. E. Ash, Probing the Role of the Hyper-Reactive Histidine Residue of Arginase, Arch. Biochem. Biophys. 444, 15-26 (2005).
• C. R. Faehnle, J. F. Ohren, and R. E. Viola, A New Branch in the Family. Structure of Aspartate β-semialdehyde Dehydrogenase from Methanococcus jannaschii, J. Molec. Biol. 353, 1055-1068 (2005).
• C. R. Faehnle, J. Blanco, and R. E. Viola, Structural Basis for Discrimination Between Oxyanion Substrates or Inhibitors in Aspartate-β-semialdehyde Dehydrogenase, Acta. Cryst. D60, 2320-2324 (2004).
• J. Blanco, R. A. Moore, C. R. Faehnle, and R. E. Viola, Critical Catalytic Functional Groups in the Mechanism of Aspartate-β-semialdehyde Dehydrogenase, Acta. Cryst. D60, 1808-1815 (2004).
• E. Cama, S. Pethe, J.-L. Boucher, S. Han, F. A. Emig, D. E. Ash, R. E. Viola, D. Mansuy, and D. W. Christianson, Inhibitor Coordination Interactions in the Binuclear Manganese Cluster of Arginase, Biochemistry 43, 8987-8999 (2004).
• J. Blanco, R. A. Moore, C. R. Faehnle, D. M. Coe, and R. E. Viola, The Role of Substrate Binding Groups in the Mechanism of Aspartate-β-semialdehyde Dehydrogenase, Acta Cryst. D60, 1388-1395 (2004).
• C. N. Madhavarao, J. R. Moffett, R. A. Moore, R. E. Viola, M. A. A. Namboodiri, and D. M. Jacobowitz, Immunohistochemical Localization of Aspartoacylase in the Rat Central Nervous System, J. Compar. Neurol. 472, 318-329 (2004).
• S. Han and R. E. Viola, Splicing of Unnatural Amino Acids into Proteins:?A Peptide Model Study, Prot. Pept. Lett. 11, 107-114 (2004).
• I. Elder, S. Han, C. K. Tu, H. Steele, P. J. Laipis, R. E. Viola, and D. N. Silverman, Activation of Carbonic Anhydrase II by Active-Site Incorporation of Histidine Analogs, Arch. Biochem. Biophys., 421, 283-289 (2004).
• J. Blanco, R. A. Moore, and R. E. Viola, Capture of an Intermediate in the Catalytic Cycle of L-Aspartate-β-semialdehyde Dehydrogenase, Proc. Natl. Acad. Sci. USA 100, 12613-12617 (2003).
• S. Han, R. A. Moore, and R. E. Viola, A Facile Synthesis of a Difluoromethylene Analog of β-Aspartyl Phosphate as an Inhibitor of L-Aspartate-β-Semialdehyde Dehydrogenases, SynLett., 845-846 (2003).
• R. A. Moore, C. R. Faehnle, J. LeCoq, and R. E. Viola, Purification and Preliminary Characterization of Brain Aspartoacylase, Arch. Biochem. Biophys. 413, 1-8 (2003).
• J. Blanco, R. A. Moore, V. Kabaleeswaram, and R. E. Viola, A Structural Basis for the Mechanism of Aspartate-β-semialdehyde Dehydrogenase from Vibrio Cholerae, Protein Sci .12, 27-33 (2003).
• R. A. Moore, W. A. Bocik, and R. E. Viola, Expression and Purification of L-Aspartate-β-semialdehyde dehydrogenase from Infectious Microorganisms, Prot. Express. Purif . 25, 189-194 (2002).
• C. L. James and R. E. Viola, Production and Characterization of Bifunctional Enzymes.?Substrate Channeling in the Aspartate Pathway, Biochemistry 41, 3726-3731 (2002).
• C. L. James and R. E. Viola, Production and Characterization of Bifunctional Enzymes.?Domain Swapping to Produce New Bifunctional Enzymes in the Aspartate Pathway, Biochemistry 41, 3720-3725 (2002).
• S. Han, R. A. Moore, and R. E. Viola, Synthesis and Evaluation of Alternative Substrates for Arginase, Bioorg. Chem. 30, 81-94 (2002).
• K. M. Jude, S. K. Wright, C. K. Tu, D. N. Silverman, R. E. Viola, and D. W. Christianson, Crystal Structure of F65A/Y131C-methylimidazole Carbonic Anhydrase V Reveals Architectural Features of an Engineered Proton Shuttle, Biochemistry 41, 2485-2491 (2002).
• J. Blanco and R. E. Viola, Purification, crystallization and preliminary X-ray analysis of aspartokinase III from Escherichia coli, Acta Cryst. D58, 352-354 (2002).
• A. Hadfield, C. Shammes, G. Kryger, D. Ringe, G. Petsko, J. Ouyang, and R. E. Viola, Active site analysis of the potential anti-microbial target Aspartate semialdehyde dehydrogenase, Biochemistry 40, 14475-14483 (2001).
• S. Han and R. E. Viola, A Spectrophotometric Assay of Arginase, Anal. Biochem. 295, 117-119 (2001).
• J. K. Bell, H. Yennawar, S. K. Wright, R. E. Viola, and L. J. Banaszak, Structural Analysis of a Malate Dehydrogenase with a Variable Active Site, J. Biol. Chem. 276, 31156-31162 (2001).
• S. K. Wright and R. E. Viola, Alteration of the Specificity of Malate Dehydrogenase by Chemical Modulation of an Active Site Arginine, J. Biol. Chem. 276, 31151-31155 (2001).
• R. E. Viola, The Central Enzymes of the Aspartate Pathway of Amino Acid Biosynthesis, Acc. Chem. Res. 34, 339-349 (2001).
• Wright, S.K.; Kish, M.M.; Viola, R.E., From Malate Dehydrogenase to Phenyllactate Dehydrogenase: Incorporation of Unnatural Amino Acids to Generate an Improved Enzyme-Catalyzed Activity, J. Biol. Chem. , in press (2000).
• Viola, R.E. "L-Aspartase: New Tricks from an Old Enzyme", in Adv. Enzymol., Mechanism of Enzyme Action, Vol. 74, Part B, (D. Purich, ed.), 2000, pp. 295-341, Academic Press, San Diego, CA.