Q. Wang, M. Zhao, G. Parungao and R. E. Viola, Purification and Characterization of
Aspartate N-acetyltransferase: A critical enzyme in brain metabolism, Prot. Expr. Purif. 119, 11-18 (2016).
Q. Q. Li, Z. X. Mu, R. Zhao, G. Dahal, R. E.Viola, T. Liu, Q. Jin and S. Cui, Structural
Insights into the Tetrameric State of Aspartate-β-semialdehyde Dehydrogenases from
Fungal Species, Nature Sci. Rep.6, 21067 (2016).
G. Dahal and R. E. Viola, Structure of a fungal form of aspartate semialdehyde dehydrogenase
from Cryptococcus neoformans, Acta Cryst. F71, 1365-1371 (2015). [PMID: 26527262].
B. Thangavelu, P. Bhansali, and R. E. Viola, Elaboration of a Fragment Library Hit
Produces Potent and Selective Aspartate semialdehyde Dehydrogenase Inhibitors, Bioorg. Med. Chem. 23, 6622-6631 (2015).
M. Zhao, P. Bhansali, K. Niefer-Sadhwani, R. E. Viola and R. M. Blumenthal, A Surprising
Range of S-Adenosyl-l-Methionine Analogs Support Bacterial Growth and Viability, Microbiology161, 674-682 (2015).
P. Wagh, G. Parungao, R. E. Viola and I. C. Escobar, A new technique to fabricate
high-performance biologically inspired membranes for water treatment, Separ. Purif. Technol.156, 754-765 (2015).
A. G. Pavlovsky, B. Thangavelu, P. Bhansali and R. E. Viola, A Cautionary Tale of
Structure-guided Inhibitor Development against an Essential Enzyme in the Aspartate
Biosynthetic Pathway, Acta Cryst., D70, 3244-3252 (2014)
B. Thangavelu, A. G. Pavlovsky and R. E. Viola, Structure of homoserine O-acetyltransferase
from Staphylococcus aureus: the first Gram-positive ortholog structure, Acta Cryst. F70, 000-000 (2014).
N. Poddar, S. Zano, R. Natarajan, B. Yamamoto and R. E. Viola, Enhanced Brain Distribution
of Modified Aspartoacylase, Molec. Genet. Metabol. 113, 000-000 (2014).
Y. S. Wijayasinghe, R. M. Blumenthal and R. E. Viola, Producing Proficient Methyl
Donors from Alternative Substrates of S-adenosylmethionine Synthetase, Biochemistry 53, 1521-1526 (2014).
S. Zano, A. G. Pavlovsky and R. E. Viola, Structure of an Unusual S-Adenosylmethionine
Synthetase from Campylobacter jejuni, Acta Cryst. D70, 442-450 (2014).
Y. S. Wijayasinghe, A. G. Pavlovsky and R. E. Viola, Aspartoacylase Catalytic Deficiency
as the Cause of Canavan Disease: A Structural Perspective, Biochemistry53, 4970-4978 (2014).
Q. Wang and R. E. Viola, Reexamination of Aspartoacylase: Is this Human Enzyme Really
a Glycoprotein?, Arch. Biochem. Biophys. 548, 66-73 (2014).
S. Zano, Y. S. Wijayasinghe, R. Malik, J. Smith, and R. E. Viola, Relationship between
Enzyme Properties and Disease Progression in Canavan Disease, J. Inher. Metab. Dis. 36, 1-6 (2013).
S. Zano, P. Bhansali, J. Smith and R. E. Viola, Alternative Substrates Selective for
S-adenosylmethionine Synthetases from Pathogenic Bacteria, Arch. Biochem. Biophys. 536, 64-71 (2013).
A. Luniwal, L. Wang, A. G. Pavlovsky, P. W. Erhardt and R. E. Viola, Molecular Docking
and Enzymatic Evaluation to Identify Selective Inhibitors of Aspartate Semialdehyde
Dehydrogenase, Bioorg. Med. Chem. 20, 2950-2956 (2012).
A. G. Pavlovsky, X. Liu, C. R. Faehnle, N. Potente and R. E. Viola, Structural Characterization
of Selective Enzyme Inhibitors Against Members of a Homologous Enzyme Family, Chem. Biol. Drug Design79, 128-136 (2012).
B. T. Arachea, Z. Sun, N. Potente, R. Malik, D. Isailovic and R. E. Viola, Detergent
Selection for Enhanced Extraction of Membrane Proteins, Protein Express. Purif., 86, 12-20 (2012).
J. G. Sarver, J. A. Trendel, N. Bearss, L. Wang, A. Luniwal, P. W. Erhardt and R.
E. Viola, Early Stage Efficacy and Toxicology Screening for Antibiotics and Enzyme
Inhibitors, J. Biomolec. Screen. 17, 673-682 (2012).
A. S. Saribas, B. T. Arachea, M. K. White, R. E. Viola and M. Safak, Human polyomavirus
JC small regulatory agnoprotein forms highly stable dimers and oligomers: Implications
for their roles in agnoprotein function, Virology420, 51-65 (2011).
D. S. Tawfik and R. E. Viola, Arsenate replacing phosphate – alternative life chemistries
and ion promiscuity, Biochemistry50, 1128-1134 (2011).
R. E. Viola, C. R. Faehnle, J. Blanco, R. A. Moore, X. Liu, B. T. Arachea and A. G.
Pavlovsky, The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis, J. Amino Acids, vol. 2011, Article ID 352538, 11 pages, 2011. (invited review).
S. Zano, R. Malik, S. Szucs, R. Matalon, and R. E. Viola, Modification of Aspartoacylase
for Potential Use in Enzyme Replacement Therapy for the Treatment of Canavan Disease, Molec. Genet. Metabol., 102, 176-180 (2011).
G. Gao, X. Liu, A. G. Pavlovsky, and R. E. Viola, Identification of Selective Enzyme
Inhibitors by Fragment-library Screening, J. Biomolec. Screen., 15, 1042-1050 (2010).
R. Malik and R. E. Viola, Tartrate Dehydrogenase: a versatile enzyme catalyzing multiple
reactions, Acta Cryst.D66, 673-684 (2010).
B. T. Arachea, X. Liu, A. G. Pavlovsky and R. E. Viola, Expansion of the Aspartate
β-semialdehyde Dehydrogenase Family. The First Structure of a Fungal Ortholog, Acta. Cryst. D66, 205-212 (2010).
D. V. Camper and R. E. Viola, Fully Automated Protein Purification, Anal. Biochem. 393, 176-181 (2009).
S. W. Bearden, C. Sexton, J. Pare, J. M. Fowler, C. G. Arvidson, L. Yerman, R. E.
Viola, and R. R. Brubaker, Attenuated Enzootic (Pestoides) Isolates of Yersinia pestis express Active Aspartase, Microbiology155, 198-209 (2009).
X. Liu, A. Pavlovsky, and R. E. Viola, The Structural Basis for Allosteric Inhibition
of a Threonine-sensitive Aspartokinase, J. Biol. Chem. 283, 16216-16225 (2008).
R. E. Viola, L. Yerman, J. M. Fowler, C. G. Arvidson and R. R. Brubaker, A Missense
Mutation Causes Aspartase deficiency in Yersinia pestis, Microbiology 154, 1271-1280
J. Le Coq, A. Pavlovsky, R. Malik, R. Sanishvili, C. Xu, and R. E. Viola, Examination
of the Mechanism of Human Brain Aspartoacylase through the Binding of an Intermediate
Analog, Biochemistry 47, 3484-3492 (2008).
R. E. Viola, X. Liu, J. F. Ohren, and C. R. Faehnle, Structure of a redundant enzyme.
The second isoform of Aspartate β-semialdehyde dehydrogenase from Vibrio cholerae,
Acta. Cryst. D64, 321-330 (2008).
X. Liu, L. Hanson, P. Langan, and R. E. Viola, The Effect of Deuteration on Protein
Structure. A High Resolution Comparison of Hydrogenous and Perdeuterated Haloalkane
Dehalogenase, Acta Cryst D63, 1000-1008 (2007)
D. M. Coe and R. E. Viola, Assessing the Roles of Catalytic Functional Groups in the
Mechanism of Homoserine Succinyltransferase, Arch. Biochem. Biophys. 461, 211-218
J. F. Ohren, M. L. Kundracik, C. L. Borders, P. Edmiston, and R. E. Viola, Subunit
Asymmetry and Communication in Muscle Creatine Kinase, Acta Cryst. D63, 381-389 (2007).
R. E. Viola, The Impact of Structural Biology on Neurobiology, Proc. Natl. Acad. Sci. USA 104, 399-400 (2007).
C. R. Faehnle, X. Liu, A. Pavlovsky, and R. E. Viola, The Initial Step in the Archael
Aspartate Biosynthetic Pathway Catalyzed by a Monofunctional Aspartokinase, Acta Cryst.
F62 , 962-966 (2006).
C. R. Faehnle, J. Le Coq, X. Liu, and R. E. Viola, Structural Complexes of the Anti-bacterial
Target Aspartate-β-semialdehyde Dehydrogenase from Streptococcus pneumoniae, J. Biol. Chem. 281, 31031-31040 (2006).
J. Le Coq, H.-J. An, C. Lebrilla, and R. E. Viola, Characterization of Aspartoacylase:
the Brain Enzyme Responsible for Canavan Disease, Biochemistry 45, 5878-5884 (2006).
Q. Liu, J. R. Kirchhoff, C. R. Faehnle, R. E. Viola, and R. A. Hudson, A Rapid Method
for the Purification of Methanol Dehydrogenase from Methylobacterium extorquens, Prot. Express. Purif. 46, 316-320 (2006).
D. M. Colleluori, R. S. Reczknwski, F. A. Emig, E. Cama, J. D. Cox, L. R. Scolnick,
K. Compher, K. Jude, S. Han, R. E. Viola, D. W. Christianson, and D. E. Ash, Probing
the Role of the Hyper-Reactive Histidine Residue of Arginase, Arch. Biochem. Biophys. 444, 15-26 (2005).
C. R. Faehnle, J. F. Ohren, and R. E. Viola, A New Branch in the Family. Structure
of Aspartate β-semialdehyde Dehydrogenase from Methanococcus jannaschii, J. Molec. Biol. 353, 1055-1068 (2005).
C. R. Faehnle, J. Blanco, and R. E. Viola, Structural Basis for Discrimination Between
Oxyanion Substrates or Inhibitors in Aspartate-β-semialdehyde Dehydrogenase, Acta. Cryst.D60, 2320-2324 (2004).
J. Blanco, R. A. Moore, C. R. Faehnle, and R. E. Viola, Critical Catalytic Functional
Groups in the Mechanism of Aspartate-β-semialdehyde Dehydrogenase, Acta. Cryst.D60, 1808-1815 (2004).
E. Cama, S. Pethe, J.-L. Boucher, S. Han, F. A. Emig, D. E. Ash, R. E. Viola, D. Mansuy,
and D. W. Christianson, Inhibitor Coordination Interactions in the Binuclear Manganese
Cluster of Arginase, Biochemistry43, 8987-8999 (2004).
J. Blanco, R. A. Moore, C. R. Faehnle, D. M. Coe, and R. E. Viola, The Role of Substrate
Binding Groups in the Mechanism of Aspartate-β-semialdehyde Dehydrogenase, Acta Cryst.D60, 1388-1395 (2004).
C. N. Madhavarao, J. R. Moffett, R. A. Moore, R. E. Viola, M. A. A. Namboodiri, and
D. M. Jacobowitz, Immunohistochemical Localization of Aspartoacylase in the Rat Central
Nervous System, J. Compar. Neurol.472, 318-329 (2004).
S. Han and R. E. Viola, Splicing of Unnatural Amino Acids into Proteins:?A Peptide
Model Study, Prot. Pept. Lett.11, 107-114 (2004).
I. Elder, S. Han, C. K. Tu, H. Steele, P. J. Laipis, R. E. Viola, and D. N. Silverman,
Activation of Carbonic Anhydrase II by Active-Site Incorporation of Histidine Analogs,
Arch. Biochem. Biophys., 421, 283-289 (2004).
J. Blanco, R. A. Moore, and R. E. Viola, Capture of an Intermediate in the Catalytic
Cycle of L-Aspartate-β-semialdehyde Dehydrogenase, Proc. Natl. Acad. Sci. USA100, 12613-12617 (2003).
S. Han, R. A. Moore, and R. E. Viola, A Facile Synthesis of a Difluoromethylene Analog
of β-Aspartyl Phosphate as an Inhibitor of L-Aspartate-β-Semialdehyde Dehydrogenases,
SynLett., 845-846 (2003).
R. A. Moore, C. R. Faehnle, J. LeCoq, and R. E. Viola, Purification and Preliminary
Characterization of Brain Aspartoacylase, Arch. Biochem. Biophys.413, 1-8 (2003).
J. Blanco, R. A. Moore, V. Kabaleeswaram, and R. E. Viola, A Structural Basis for
the Mechanism of Aspartate-β-semialdehyde Dehydrogenase from Vibrio Cholerae, Protein Sci .12, 27-33 (2003).
R. A. Moore, W. A. Bocik, and R. E. Viola, Expression and Purification of L-Aspartate-β-semialdehyde
dehydrogenase from Infectious Microorganisms, Prot. Express. Purif . 25, 189-194 (2002).
C. L. James and R. E. Viola, Production and Characterization of Bifunctional Enzymes.?Substrate
Channeling in the Aspartate Pathway, Biochemistry41, 3726-3731 (2002).
C. L. James and R. E. Viola, Production and Characterization of Bifunctional Enzymes.?Domain
Swapping to Produce New Bifunctional Enzymes in the Aspartate Pathway, Biochemistry41, 3720-3725 (2002).
S. Han, R. A. Moore, and R. E. Viola, Synthesis and Evaluation of Alternative Substrates
for Arginase, Bioorg. Chem.30, 81-94 (2002).
K. M. Jude, S. K. Wright, C. K. Tu, D. N. Silverman, R. E. Viola, and D. W. Christianson,
Crystal Structure of F65A/Y131C-methylimidazole Carbonic Anhydrase V Reveals Architectural
Features of an Engineered Proton Shuttle, Biochemistry41, 2485-2491 (2002).
J. Blanco and R. E. Viola, Purification, crystallization and preliminary X-ray analysis
of aspartokinase III from Escherichia coli, Acta Cryst.D58, 352-354 (2002).
A. Hadfield, C. Shammes, G. Kryger, D. Ringe, G. Petsko, J. Ouyang, and R. E. Viola,
Active site analysis of the potential anti-microbial target Aspartate semialdehyde
dehydrogenase, Biochemistry40, 14475-14483 (2001).
S. Han and R. E. Viola, A Spectrophotometric Assay of Arginase, Anal. Biochem.295, 117-119 (2001).
J. K. Bell, H. Yennawar, S. K. Wright, R. E. Viola, and L. J. Banaszak, Structural
Analysis of a Malate Dehydrogenase with a Variable Active Site, J. Biol. Chem.276, 31156-31162 (2001).
S. K. Wright and R. E. Viola, Alteration of the Specificity of Malate Dehydrogenase
by Chemical Modulation of an Active Site Arginine, J. Biol. Chem.276, 31151-31155 (2001).
R. E. Viola, The Central Enzymes of the Aspartate Pathway of Amino Acid Biosynthesis,
Acc. Chem. Res.34, 339-349 (2001).
Wright, S.K.; Kish, M.M.; Viola, R.E., From Malate Dehydrogenase to Phenyllactate
Dehydrogenase: Incorporation of Unnatural Amino Acids to Generate an Improved Enzyme-Catalyzed
Activity, J. Biol. Chem. , in press (2000).
Viola, R.E. "L-Aspartase: New Tricks from an Old Enzyme", in Adv. Enzymol., Mechanism of Enzyme Action, Vol. 74, Part B, (D. Purich, ed.), 2000, pp. 295-341, Academic Press, San Diego, CA.