Department of Pharmacology : Ming-Cheh Liu

Institutions/Degrees

• The cytosolic sulfotransferases (SULTs)
• Drug metabolism
• Developmental Pharmacology
• Oxidative/nitrative Stress
• Tyrosylprotein sulfotransferases (TPSTs)
• Protein tyrosine sulfation
• Zebrafish model

Selected Publications

• Liu, T.-A., Yasuda, S., Williams, F.E., Liu, M.-Y., Suiko, M., Sakakibara, Y., Yang, Y.-S., and Liu, M.-C., “A Target-specific Approach for the Identification of Tyrosine-sulfated Hemostatic Proteins,” Anal. Biochem. (2009) In Press.

• Yasuda, S., Yasuda, T., Hui, Y, Liu, M.-Y., Suiko, M., Sakakibara, Y., and Liu, M.-C., “Concerted Action of the Cytosolic Sulfotransferase, SULT1A3, and Catechol-O-Methyltransferase in the Metabolism of Dopamine in SK-N-MC Human Neuroblastoma Cells,” Neurosci. Res. (2009) In Press.

• Teramoto, T., Sakakibara, Y., Liu, M.-C., Suiko, M., Kimura, M., and Kakuta, Y., “Snapshot of a Michaelis complex in a sulfuryl transfer reaction: Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate,” Biochem. Biophys. Res. Commun. (2009) In Press.

  Zhang, P., Liu, M.-C., Cheng, L., Liang, M., Ji, H.-L., and Fu, J., “Blockade of LOX-1 prevents endotoxin-induced acute lung inflammation and injury in mice,” J. Innate. Immunity (2009) In Press.
• Teramoto, T., Sakakibara, Y., Liu, M.-C., Suiko, M., Kimura, M., and Kakuta, Y., “Structural basis for the broad range substrate specificity of a novel mouse cytosolic sulfotransferase - mSULT1D1,” Biochem. Biophys. Res. Commun. (2009) 379, 76-80.
• Liu, T.-A., Bhuiyan, S., Snow, R., Yasuda, S., Yasuda, T., Yang, Y.-S., Williams, F.E., Liu, M.-Y., Suiko, M., Carter, G., and Liu, M.-C., “Identification and Characterization of Two Novel Cytosolic Sulfotransferases, SULT1 ST7 and SULT1 ST8, from Zebrafish,” Aquat. Toxicol. (2008) 89, 94-102.
• Teramoto, T., Sakakibara, Y., Inada, K., Kurogi, K., Liu, M.-C., Suiko, M., Kimura, M., and Kakuta, Y., “Crystal structure of mSULT1D1, a mouse catecholamine sulfotransferase,” FEBS Lett. (2008) 582, 3909-3914.
• Yasuda, T., Yasuda, S., Williams, F.E., Liu, M.-Y., Sakakibara, Y., Bhuiyan, S., Snow, R., Carter, G., and Liu, M.-C., “Characterization and Ontogenic Study of Novel Steroid-sulfating SULT3 Sulfotransferases from Zebrafish,” Mol. Cell. Endocrinol. (2008) 294, 29-36.
• Takahashi, S., Sakakibara, Y., Mishiro, E., Kouriki, H., Nobe, R., Kurogi, K., Yasuda, S., Liu, M.-C., and Suiko, M., “Molecular Cloning, Expression, and Characterization of Mouse Amine N-Sulfotransferases,” Biochem. Biophys. Res. Commun. (2008) 375, 531-535.
• Hui, H., Yasuda, S., Liu, M.-Y., Wu, Y.-Y., and Liu, M.-C., “On the Sulfation and Methylation of Catecholestrogens in Human Mammary Epithelial Cells and Breast Cancer Cells,” Biol. Pharm. Bull. (2008) 31, 769-773.
• Velusamy, A., Shetty, P., Bhandary, Y.P., Liu, M.-C., Shetty, S., “Posttranscriptional Regulation of Urokinase Receptor Expression by Heterogeneous Nuclear Ribonuclear Protein C,” Biochemistry (2008) 47, 6508-6517.
• Shetty, P., Velusamy, A., Bhandary, Y.P., Shetty, R.S., Liu, M.-C., and Shetty, S., “Urokinase Expression by Tumor Suppressor Protein p53: A Novel Role in mRNA Turnover,” Am. J. Resp. Cell. Mol. Biol. (2008) 39, 364-372.
• Liu, T.-A., Liu, M.-C., and Yang, Y.-S., “Immunohistochemical Analysis of A Novel Dehydroepiandrosterone Sulfotransferase-like Protein in Drosophila Neural Circuit,” Biochem. Biophys. Res. Commun. (2008) 367, 14-20.
• Yasuda, S., Liu, M.-Y., Suiko, M., Sakakibara, Y., and Liu, M.-C., “Hydroxylated Serotonin and Dopamine as Substrates and Inhibitors for Human Cytosolic SULT1A3,” J. Neurochem. (2007) 103, 2679-2689.
• Liu, M.-C., Yasuda, S., and Idell, S., "Sulfation of Nitrotyrosine: Biochemistry and Functional Implications," IUBMB Life (2007) 59, 622-627.
• Yasuda, S., Idell, S., Fu, J., Carter, G., Snow, R., and Liu, M.-C., "Cigarette Smoke Toxicants as Substrates and Inhibitors for Human Cytosolic SULTs," Toxicol. Appl. Pharmacol. (2007) 221, 13-20.
• Yasuda, S., Idell, S., and Liu, M.-C., "Generation and Release of Nitrotyrosine O-Sulfate by HepG2 Human Hepatoma Cells upon SIN-1 Stimulation: Identification of SULT1A3 as the Responsible Enzyme," Biochem. J. (2007) 401, 497-503.
• Yasuda, S., Liu, M.-Y., Yang, Y.-S., Snow, R., Takahashi, S., and Liu, M.-C., "Identification of Novel Hydroxysteroid-sulfating Cytosolic SULTs, SULT2 ST2 and SULT2 ST3, from Zebrafish: Cloning, Expression, Characterization, and Ontogenic Study," Arch. Biochem. Biophys. (2006) 455, 1-9.
• Mishiro, E., Sakakibara, Y., Liu, M.-C., and Suiko, M., "Differential Enzymatic Characteristics and Tissue-specific Expression of Human TPST-1 and TPST-2," J. Biochem. (Tokyo) (2006) 140, 731-737.
• Yasuda, S., Kumar, P., Liu, M.-Y., Sakakibara, Y., Suiko, M., Chen, L., and Liu, M.-C., "Identification of A Novel Thyroid Hormone-sulfating Cytosolic SULT, SULT1 ST5, from Zebrafish: Molecular Cloning, Expression, Characterization, and Ontogenic Study," FEBS J. (2005) 272, 3828-3837.
• Suiko, M., Sakakibara, Y., Liu, M.-Y., Yang, Y.-S., and Liu, M.-C., "Cytosolic SULTs and Environmental Estrogenic Chemicals," J. Pesticide Sci. (2005) 30, 345-353.
• Lu., J.H., Li, H.T., Liu, M.-C., Zhang, J.P., Li, M., An, X.M., and Chang, W.-R., "Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3�-Phosphoadenosine 5�-Phosphate," Biochem. Biophys. Res. Commun. (2005) 335, 417-423.
• Shetty, S., Ganachari, M., Liu, M.-C., Azghani, A., Muniyappa, H., and Idell, S., "Regulation of Urokinase Receptor Expression by Phosphoglycerate Kinase is Independent of Its Catalytic Activity," Am. J. Physiol. Lung Cell. Physiol. (2005) 289, L591-598.
• Liu, M.-Y., Yang, Y.-S., Sugahara, T., Yasuda, S., and Liu, M.-C., "Identification of A Novel Zebrafish SULT1 Cytosolic Sulfotransferase: Cloning, Expression, Characterization, and Developmental Expression Study," Arch. Biochem. Biophys. (2005) 437, 10-19.
• Yasuda, S., Liu, C.-C., Takahashi, S., Suiko, M., Chen, L., Snow, R., and Liu, M.-C., "Identification of A Novel Estrogen-sulfating Cytosolic SULT from Zebrafish: Molecular Cloning, Expression, Characterization, and Ontogeny Study," Biochem. Biophys. Res. Commun. (2005) 330, 219-225.
• Yasuda, S., Suiko, M., and Liu, M.-C., "Oral Contraceptives as Substrates and Inhibitors for Human Cytosolic SULTs," J. Biochem. (Tokyo) (2005) 137-401-406.
• Chen, W.T., Liu, M.-C., and Yang, Y.-S., "Fluorometric Assay for Alcohol Sulfotransferase," Anal. Biochem. (2005) 339, 54-60.
• Ohkimoto, K., Sakakibara, Y., Suiko, M., Yoshikawa, H., Liu, M.-C., and Tamura, H., "Biocides, Tributyltin and Triphenyltin, as Possible Inhibitors of the Human Sulfotransferase Involved in the Estrogen Homeostasis," Pesticide Biochem. Physiol. (2005) 81, 32-38.
• Mishiro, E., Liu, M.-Y., Sakakibara, Y., Suiko, M., and Liu, M.-C., "Zebrafish Tyrosylprotein Sulfotransferase: Molecular Cloning, Expression, and Functional Characterization," Biochem. Cell Biol. (2004) 82, 295-303.
• Ohkimoto, K., Liu, M.-Y., Sakakibara, Y., Suiko, M., and Liu, M.-C., "Characterization of A Zebrafish Estrogen-sulfating Cytosolic Sulfotransferase: Inhibitory Effects and Mechanism of Action of Phytoestrogens," Chemico-Biological Interactions (2004) 147, 1-7.
• Pai, T.G., Oxendine, I., Sugahara, T., Suiko, M., Sakakibara, Y., and Liu, M.-C., "Structure-Function Relationships in the Stereospecific and Manganese-Dependent Dopa/Tyrosine-Sulfating Activity of Human Monoamine-Form Phenol Sulfotransferase, SULT1A3," J. Biol. Chem. (2003) 278, 1525-1532.
• Sugahara, T., Yang, Y.-S., Liu, C.-C., Pai, T.G., and Liu, M.-C., "Sulphonation of Dehydroepiandrosterone and Neurosteroids: Molecular Cloning, Expression, and Functional Characterization of A Novel Zebrafish SULT2 Cytosolic Sulphotransferase," Biochem. J. (2003) 375, 785-791.
• Ohkimoto, K., Sugahara, T., Sakakibara, Y., Suiko, M., Liu, M.-Y., Carter, G., and Liu, M.-C., "Sulfonation of Environmental Estrogens by Zebrafish Cytosolic Sulfotransferases," Biochem. Biophys. Res. Commun. (2003) 309, 7-11.
• Sugahara, T., Liu, C.-C., Pai, T.G., Collodi, P., Suiko, M., Sakakibara, Y., Nishiyama, K., and Liu, M.-C., "Sulfation of Hydroxychlorobiphenyls: Molecular Cloning, Expression, and Functional Characterization of Zebrafish SULT1 Sulfotransferases," Eur. J. Biochem. (2003) 270, 2404-2411.
• Sugahara, T., Liu, C.-C., Carter, G., Pai, T.G., and Liu, M.-C., "cDNA Cloning, Expression, and Functional Characterization of A Zebrafish SULT1 Cytosolic Sulfotransferase," Arch. Biochem. Biophys. (2003) 414, 67-73.
• Sugahara, T., Pai, T.G., Suiko, M., Sakakibara, Y., and Liu, M.-C., "Differential Roles of Human Monoamine (M)-form and Simple Phenol (P)-form Phenol Sulfotransferases in Drug Metabolism," J. Biochem. (Tokyo) (2003) 133, 259-262.
• Sugahara, T., Liu, C.-C., Pai, T.G., and Liu, M.-C., "Molecular Cloning, Expression, and Functional Characterization of A Novel Zebrafish Cytosolic Sulfotransferase," Biochem. Biophys. Res. Commun. (2003) 300, 725-730.
• Pai, T.G., Sugahara, T., Suiko, M., Sakakibara, Y., Xu, F., and Liu, M.-C., "Differential xenoestrogen-sulfating activities of the human cytosolic sulfotransferases: molecular cloning, expression, and purification of human SULT2B1a and SULT2B1b sulfotransferases," Biochim. Biophys. Acta (2002) 1573, 165-170.
• Xu, F., Suiko, M., Sakakibara, Y., Pai, T.G., and Liu, M.-C., "Regulatory effects of divalent metal cations on human cytosolic sulfotransferases," J. Biochem. (Tokyo) (2002) 132, 457-462.
• Sakakibara, Y., Suiko, M., Pai, T.G., Nakayama, T., Takami, Y., Katafuchi, J., and Liu, M.-C., "Highly conserved mouse and human brain sulfotransferases: molecular cloning, expression, and functional characterization," Gene (2002) 285, 39-47.
• Pai, T.G., Ohkimoto, K., Sakakibara, Y., Suiko, M., Sugahara, T., and Liu, M.-C., "Manganese Stimulation and Stereospecificity of the Dopa (3,4-Dihydroxyphenylalanine)/Tyrosine-sulfating Activity of Human Monoamine-form Phenol Sulfotransferase. Kinetic Studies of the Mechanism using Wild-type and Mutant Enzymes," J. Biol. Chem. (2002) 277, 43813-43820.