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Health Science Campus
Block Health Science Building

Mail Stop # 1007
3000 Arlington Avenue
Toledo, Ohio 43614-2598
Phone: 419.383.4109
Fax: 419.383.3008

David R. Giovannucci, Ph.D.

Associate Professor
Director, Raymond & Beverly Sackler Laboratory for Neuroendocrine Tumor Research

Office: 105 Block Health Sciences Building

Tel: 419-383-5004

Lab: 419-383-4023

Fax: 419-383- 3008

Ph.D., 1993, Wayne State University, Detroit, MI.
Postdoctoral Fellow, 1993-1998, University of Michigan Medical School, Ann Arbor, MI
Research Assistant Professor, 1999-2002, University of Rochester Medical Center, Rochester, NY.

Research Interests:

Cellular and molecular basis of peptide and protein secretion; Ca 2+ signaling mechanisms; Neuroendocrine cancer; Mitochondrial function in health and disease.

Research Techniques:
Electrophysiology; Confocal and multiphoton microscopy; Molecular biology and protein biochemistry.
Research Summary:

The exocytotic secretion of peptide neurotransmitters and hormones has wide-ranging consequences for neuronal function, cardiovascular homeostasis and GI tract regulation. Work in our lab is focused on how intracellular levels of Ca 2+, ATP and cyclic AMP control the release of vasoactive molecules. This is achieved using a combination of electrophysiological and optical methods to follow in real-time the secretory activity and Ca 2+ dynamics of single isolated nerve endings and a variety of acutely isolated or cultured cell types.

References (partial list):

1.Giovannucci DR & Stuenkel EL. (1995) Glutamate receptor agonists modulate [Ca2+]i in isolated rat melanotropes. Neuroendocrinology 62, 111-122.1.

2.Giovannucci DR & Stuenkel EL. (1995) An NMDA receptor on isolated secretory nerve endings. Brain Research 702, 246-250.

3.Giovannucci DR & Stuenkel EL. (1997) Regulation of secretory granule recruitment and exocytosis at rat neurohypophysial nerve endings. Journal of Physiology 498.3, 735-751.

4.Rusin KI, Giovannucci DR , Stuenkel EL & Moises HC. (1997) k-Opioid receptor activation modulates Ca2+ currents and secretion in isolated neuroendocrine nerve terminals. Journal of Neuroscience 17(17): 6565-74.

5.Giovannucci DR, Yule, D.I. & Stuenkel EL (1998) Optical measurement of stimulus-evoked membrane turnover in single pancreatic acinar cells. American Journal of Physiology. (3 Pt 1):C732-9.

6.Giovannucci DR & Stephenson RS. (1999) Identification and distribution of dietary precursors of the Drosophila visual pigment chromophore: analysis of carotenoids in wild type and ninaD mutants by HPLC. Vision Research 39(2):219-29.

7.Fletcher AI, Shuang R, Giovannucci DR, Zhang L, Bittner M, & Stuenkel EL (1999) Regulation of exocytosis by cyclin-dependent kinase 5 via phosphorylation of Munc18. Journal of Biological Chemistry 274(7):4027-35.

8.Giovannucci DR, Hlubek MD, & Stuenkel EL. (1999) Mitochondria Regulate the Ca2+-exocytosis Relationship of Bovine Adrenal Chromaffin Cells. Journal of Neuroscience 19(21): 9261-9270.

9.Giovannucci DR , Sneyd J, Groblewski GE & Yule DI. (2000) Modulation of InsP3 receptor properties by phosphorylation. J Korean Med. Sci. S55-6.

10.Giovannucci DR , Sneyd J, Groblewski GE & Yule DI. (2000) Targeted phosphorylation of the inositol trisphosphate receptor selectively inhibits localized Ca2+ release and shapes oscillatory Ca2+ signals. Journal of Biological Chemistry 275: 33704–33711.

11.Giovannucci DR , Straub & Yule, DI. (2000) Calcium wave propagation in pancreatic acinar cells: functional interaction of inositol trisphosphate receptors, ryanodine receptors, and mitochondria. Journal of General Physiology 116 :547–559.

12.Beutner G, Sharma VK, Giovannucci DR, Yule DI, & Sheu S-S. (2001) Identification of a ryanodine receptor in rat heart mitochondria. Journal of Biological Chemistry 276(24): 21482-8.

13.Bruce JIE, Shuttleworth TJ, Giovannucci DR & Yule DI. (2002) Phosphorylation of inositol 1,4,5-trisphosphate receptors: a mechanism for the synergistic effects of cAMP on Ca2+ signaling. Journal of Biological Chemistry 277(2), 1340-1348.

14.Giovannucci DR, Bruce JIE, Straub SV, Arreola J, Shuttleworth TJ & Yule DI. Cytosolic Ca2+ and Ca2+-activated Cl- current dynamics: insights from two functionally distinct exocrine cells. Journal of Physiology (2002) 540.2: 469-484.

15.Straub SV, Giovannucci DR *, Bruce JI & Yule DI. A role for phosphorylation of inositol 1,4,5-trisphosphate receptors in defining calcium signals induced by Peptide agonists in pancreatic acinar cells. (2002) Journal of Biological Chemistry, 277(35): 31949-56.

16.Sneyd J, Tsaneva-Atanasova K, Bruce JI, Straub SV, Giovannucci DR, Yule DI. (2003) A model of calcium waves in pancreatic and parotid acinar cells. Biophys J. 85(3):1392-405.

17.Soldo BL, Giovannucci DR *, Stuenkel EL, Moises HC. (2004) Ca2+ and frequency dependence of exocytosis in isolated somata of magnocellular supraoptic neurones of the rat hypothalamus. J Physiol. 16;555(Pt 3):699-711.

18.Bruce JI, Giovannucci DR , Blinder G, Shuttleworth TJ, Yule DI. (2004) Modulation of [Ca2+]i signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells. J Biol Chem. 279(13):12909-17.

19.Chen Y, Warner JD, Yule DI, Giovannucci DR. (2005) Spatiotemporal Analysis of Exocytosis in Mouse Parotid Acinar Cells. American Journal of Physiology (Cell), published on-line ahead of print.

20.Yuan Z, Cai T, Tian J, Ivanov AV, Giovannucci DR , Xie Z. (2005) Na/K-ATPase Tethers Phospholipase C and IP3 Receptor into a Calcium-regulatory Complex. Mol Biol Cell. 2005 16(9): 4034-45.

21.Mignen O, Brink C, Enfissi A, Nadkarni A, Shuttleworth TJ, Giovannucci DR, Capiod, T. (2005) Carboxyamidotriazole-induced inhibition of mitochondrial calcium import blocks capacitative calcium entry and cell proliferation in HEK 293 cells. J Cell Sci. 2005 118(Pt 23): 5615-23.

22.Peters CG, Miller DF, Giovannucci DR. (2006) Identification, localization and interaction of SNARE proteins in atrial cardiac myocytes, Journal of Molecular and Cellular Cardiology 40(3): 361-374.

Last Updated: 6/26/15