University of Toledo

Training - Online Registration Only

You must now register for the training classes online. Go to the UT Environmental Health and Radiation Safety website and log in. Look for the DLAR classes on the calendar. Everyone must be approved to work on an animal protocol.

If you are being added to an existing protocol, a personnel amendment must be filled out and sent to DLAR before your training begins.

Personnel Amendment form


Orientation 1hr (Mandatory for anyone using animals in research):  This session covers regulatory compliance, occupational health, IACUC policies, DLAR procedures and what you need to know about the privilege of using animals in research. 

       Welcome Orientation to DLAR Researcher

SPF/Conventional/Rodent Breeding 1 hr (directly following orientation): This session is incorporated in the orientation. Review policies and procedures, including traffic flow, access, etc. We also review rodent breeding schemes, how to handle difficult breeding strains, identification and tissue collection for genotyping. Directly following we do a facility tour and review micro-isolator techniques using a laminar flow hood.

Wet Labs & Technical Skills 2-3 hrs: There are two separate wet labs - mouse and rat. Basic rodent handling, injections, euthanasia, length of session depends on experience and number of trainees per session. This session must be completed prior to Surgery training (if applicable).

Some helpful links: Here is a link to great videos on technical procedures in mice and rats. Feel free to watch these videos prior to training and also for refreshers after training. (Rodent Handling and Restraint Techniques, Basic Care Procedures, Fundamentals of Breding and Weaning, and Rodent Identification.)

Technical Skills (are protocol specific and taught during the Wet Lab): This training is protocol specific (e.g. Submandibular Bleeding, Gavage, Retro Orbital (RO) Injections, Bleeding and Tail Vein Injections, Tail nick, etc.)

Some helpful links: Here is a link to great videos on technical procedures in mice and rats. Feel free to watch these videos prior to training and for refreshers after training. (Compound Administration I, II, III, Blood Withdrawal I & II, Anesthesia Induction and Maintenance, Considerations for Rodent Surgery.)

Aseptic Technique Wet Lab - 3 hrs:  This session covers aseptic technique, instrument preparation, pre- and post-op care. If you will not be performing surgery you are not required to attend this session. In preparation for the lab, you are required to watch the following videos and review the article below prior to the training session.

     Rodent Survival Surgery -

     Rodent skin prep -

     Open glove technique - the first 3 minutes -

     Please review the article and forms below prior to the training session:

          Guidelines for Rodent Survival Surgery

          Surgery Room Set-up

          Surgery Steps Checklist

          UCSF Rodent Surgery Anesthesia Record

Contact the DLAR office at 419-383-4310 for any questions. 

Last Updated: 6/29/18