University of Toledo



You must be on an existing approved protocol or be in the process of being added to an existing protocol by means of a personnel amendment. The personnel amendment must be sent to DLAR ( before your training begins.

     Click here for a blank: Personnel Amendment form

What training do you need?                hand  

Vivarium Orientation (2 hrs and mandatory for anyone using animals in research):  This session covers regulatory compliance, occupational health, IACUC policies, DLAR procedures and what you need to know about the privilege of using animals in research. We review policies and procedures, including traffic flow, access. etc. We outline the difference between Specific Pathogen Free "SPF", Conventional and Containment housing. There is an overview of rodent breeding schemes, how to handle difficult breeding strains, and identification and tissue collection for genotyping. Directly following we do a facility tour and review micro-isolator techniques using a laminar flow hood.

     Preview of the PowerPoint for Vivarium Orientation: Vivarium Orientation

Wet Labs & Technical Skills(3 hrs and mandatory for anyone using animals in research): There are two separate wet labs. One specific to the mouse and a separate one specific to the rat. Sign up for the one you need or both if you are working with mice and rats. The wet labs cover basic rodent handling, injections, euthanasia, length of session depends on experience and number of trainees per session. We also cover if needed submandibular bleeding, oral gavage, tail vein injections, tail nick, etc.

     Review videos prior to wet lab, (Rodent Handling and Restraint Techniques, Basic Care Procedures, Fundamentals of Breeding and Weaning, and Rodent Identification.) Videos for Wet Lab and Technical Skills -

     This session is a prerequisite for Aseptic Technique Wet Lab (if applicable).


Isoflurane Anesthesia (1 hr): This session is required for anyone performing isoflurane anesthesia for procedures or surgery on an IACUC protocol. We will cover the setup of the equipment, anesthesia induction and maintenance, monitoring, and post-anesthesia supportive care.

     This session is a prerequisite for Aseptic Techqnique Wet Lab (if applicable).


Aseptic Technique Wet Lab (3 hrs): Must have taken "Wet Lab & Technical Skills" as well as "Isoflurane Anesthesia" before signing up for this class. This session covers aseptic technique, instrument preparation, pre- and post-op care. If you will not be performing surgery you should not attend this session. You must review video and articles prior to wet lab.

     Rodent Survival Surgery -

     Rodent skin prep -

     Gloving Techniques (first 3 min of video) -

     Guidelines for Rodent Survival Surgery

     Surgery Room Set-up

     Surgery Steps Checklist

     UCSF Rodent Surgery Anesthesia Record

Contact the DLAR office at 419-383-4310 for any questions. 



Last Updated: 9/7/18