Pulmonary Lab - Principal Investigator, James C. Willey, M.D.
Dr. James C. Willey, M.D.
University of Vermont, Burlington, VT B.A. 1974 Zoology
Medical College of Ohio, Toledo, OH M.D. 1978 Medicine
Present Laboratory Personnel
Vrushalee Palsule, M.S.
Thomas Blomquist, B.S. (M.D./Ph.D. Candidate)
Lauren Stanoszek, B.S. (M.D./Ph.D. Candidate)
Jiyoun Yeo, (Ph.D. Candidate)
Xiaolu Zhang (Ph.D. Candidate)
Lindsey Pierce (Ph.D. Candidate)
James Willey, M.D.
Graduated Former Students
D'Anna Mullins, M.D., Ph.D.
Yasser Aldhamen, M.S.
Timothy Graves, Ph.D.
Former Post-Doctoral Fellows
Kristy Warner, Ph.D., 2000-2003
Former MS and BS Lab Members
Charles Knight, M.S., 2002-2005
Active NIH Grants
United States Patent # 5,639,606
United States Patent # 5,643,765
United States Patent # 5,876,978
U.S. Patent No. # 7,476,502
U.S. Patent No. # 7,527,930
Methods And Compositions For Identifying Cancer-Related Biomarkers 20070092893 Willey; James C. ; et al. April 26, 2007
Compositions and methods of use of standardized mixtures 20060188908 Willey; James C. ; et al. August 24, 2006
Methods and compositions for assessing nucleic acids 20060183144 Willey; James C. ; et al. August 17, 2006
Method and compositions for the diagnosis and treatment of non-small cell lung cancer
using gene expression profiles 20050260586 Demuth, Jeffrey P. ; et al. November 24, 2005
Method for quantitative measurement of gene expression for indentifying individuals
at risk for bronchogenic carcinoma 20040197785 Willey, James C. ; et al. October 7, 2004
Blomquist, T. Crawford, E.L., Mullins, D., Yoon, Y., Hernandez, D., Khuder, S., Ruppel, P.L., Peters, E., Oldfield, D.J., Austermiller, B., Anders, J.C., and Willey, J.C., Pattern of antioxidant and DNA repair gene expression in normal airway epithelium associated with lung cancer diagnosis, Cancer Research, 69, 8629-8635, 2009.
Blomquist T., Crawford, E. L. and Willey, J.C., Genetic variation at E2F1/YY1 and P53 consensus sites is associated with altered allele-specific expression of ERCC5 (XPG) transcript in normal human bronchial epithelium, Carcinogenesis, 31, 1242-1250, 2010.
MAQC Consortium, The MicroArray Quality Control (MAQC)-II study of common practices for the development and validation of microarray-based predictive models, Nature Biotechnology, 28, 827-838, 2010.
Crawford, E. L., Blomquist, T, Mullins, D.N., Yoon, Y., Hernandez, D.R., Al-Bagdhadi,M.,
Ruiz,J., Hammersley, J., Willey, J.C., CEBPG regulates ERCC5/XPG expression in human
bronchial epithelial cells and this regulation is modified by E2F1/YY1 interactions,
Carcinogenesis, doi:10.1093/carcin/bgm214, 2007.
Braun DP, Ding JD, Shaheen F, Willey, JC, Rana N, and Dmowski WP. Quantitative Expression of Apoptosis Regulating Genes in Endometrium from Women with and without Endometriosis. Fertil and Steril., 87, 263-8, 2007.
Canales, R.D.*, Luo, Y.*, Willey, J.C.*, et al, (Canales, Luo, Willey Shared lead). Evaluation of DNA Microarray Results with Alternative Quantitative Technology Platforms, Nature Biotechnology, 24, 1115-1122, 2006. (* equal contributors)
Leming Shi*, Reid, L. H.*, Jones, W. D.*, Shippy, R.*, Warrington, J. A.*, Baker, S. C.*, Collins, P. J.*, de Longueville, F.*, Kawasaki, E. S.*, Lee, K. Y.*, Luo, Y.*, Sun, Y. A.*,
Willey, J. C.* et al, The MicroArray Quality Control (MAQC) project shows inter- and intra-platform reproducibility of gene expression measurements, Nature Biotechnology, 24, 1151-1161, 2006. (*These listed authors were project managers)
Shippy, R., Wolber, P., Jones, W.D., Jensen, R.V., Johnson, C.D., Guo, X., Chudin, E., Fulmer-Smentek, Boysen, S., C., Pine, S.P., Sun Y., Willey, J.C. et al, The use of RNA sample titrations for assessing microarray performance and normalization techniques, Nature Biotechnology, 24, 1123-1131, 2006.
Peters, GJ, van Moorsel, CJA, Lakerveld, B, Smid, K, Noordhuis, P, Comijn, EC, Weaver, D, Willey, JC, Voorn, D, Van der Vijgh, WJF, Pinedo, HM, Effects of gemcitabine on cis-platinum-dna adduct formation and repair in a panel of gemcitabine and cisplatin sensitive or resistant human ovarian cancer cell lines, Int J Oncology, 28, 237-44, 2006.
Sudoa, H, Li-Sucholeiki, X-C, Marcelino, LA, Gruhl, AN, Zarbl, H, Willey, JC, Thilly, WG, Distributions of five common point mutants in the human tracheal-bronchial epithelium, Mutation Research, 596, pages 113-127, 2006.
Graves, TG, Harr, MW, Crawford, EL, Willey, JC, Stable low-level expression of p21WAF1/CIP1in A549 human bronchogenic carcinoma cell line-derived clones down-regulates E2F1 mRNA and restores cell proliferation control, Molecular Cancer, 5, 1, 2006.
Mullins, DN, Crawford, EL, Khuder, SA, Hernandez, D-A, Yoon, Y, Willey, JC, CEBPG transcription factor correlates with antioxidant and DNA repair genes in normal bronchial epithelial cells but not in individuals with bronchogenic carcinoma, BMC Cancer, 5, 141, 2005.
Harr, MW, Graves, TG, Crawford, EL, Warner, KA, Reed, CAM, and Willey, JC, Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21waf1/cip1 among human bronchogenic carcinomas, Molecular Cancer, 4, 23, 2005.
Weaver, DA, Crawford, EL., Warner, KA, Elkhairi, F, Khuder, SA, and Willey, JC, Association of ABCC5, ERCC2, XPA, and XRCC1 gene expression with cisplatin chemoresistance in non-small cell lung cancer cell lines, Molecular Cancer, 4, 18, 2005.
Willey JC, Crawford EL, Knight CR, Warner KA, Motten CA, Herness EA, Zahorchak RJ, Graves TG., Standardized RT-PCR and the standardized expression measurement center, Methods Mol Biol. 2004, 258, 13-41.
Pagliarulo V, George B, Beil SJ, Groshen S, Laird PW, Cai, J, Willey J, Cote RJ, Datar RH. Related Articles, Links Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR, Mol Cancer, 3, 5, 2004.
Warner KA, Crawford EL, Zaher A, Roshong-Denk SL, Sharief I, Amurao GV, Coombs RJ, Elsamaloty H, Yoon Y, Al-Astal AY, Jboor N, Assaly RA, Graves TG, Knight CR, Harr MW, Sheridan TB, DeMuth JP, Zahorchak RJ, Hammersley JR, Olson DE, and
Willey JC, The c-myc x E2F-1/p21 Interactive gene expression index augments cytomorphologic diagnosis of lung cancer in fine needle aspirate specimens, Journal of Molecular Diagnostics, 5, 176-83, 2003.
Vondracek M., Weaver D., Sarang Z., Hedberg J.J, Willey J.C. Wärngård L., and Grafström R.C. Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and Simian virus 40 T antigen-immortalized oral keratinocytes. Int. J. of Cancer, 99, 776-782, 2002.
Crawford, EL, Warner, KA, Khuder, SA, Zahorchak, RJ, Willey, JC. Multiplex Standardized RT-PCR for expression analysis of many genes in small clinical samples. Biochem.Biophis.Res.Commun.,293,509-516, 2002.
Crawford, E.L., Godfridus, J.P., Noordhuis, P., Rots, M.G., Vondracek, M., Grafström, R.C., Lieuallen, K., Lennon, G., Zahorchak, R.J., Georgeson, M.J., Wali, A., Lechner, J.F., Fan, P.-S., Kahaleh, M.B., Khuder, S.A., Warner, K.A., Weaver, D.A., and Willey, J.C., Reproducible gene expression measurement among multiple laboratories obtained in a blinded study using standardized RT (StaRT)-PCR, Mol. Diagnosis, 6, 217-225, 2001.
Rots, M.G., Willey, J.C., Jansen, G., Van Zantwijk, C.H., Noordhuis, P., DeMuth, J.P, Kuiper, E., Veerman, A.J.P., Pieters, R. and Peters, G.J. mRNA expression levels of methotrexate resistance-related proteins in childhood leukemia as determined by a standardized competitive template-based RT-PCR method. Leukemia, 14, 2166-2175, 2000.
Crawford E. L, Khuder S. A., Durham S. J., Frampton, M., Utell, M.A., Thilly W.G., Weaver D.A., Ferencak W.J., Jennings C.A., Hammersley J.R., Olson D.A., Willey J.C. Normal bronchial epithelial cell expression of glutathione transferase P1, glutathione transferase M3, and glutathione peroxidase is low in subjects with bronchogenic carcinoma. Cancer Res. 60, 1609-18, 2000.
Willey, J.C., Crawford, E.L., Jackson, C.M., Weaver, D.A., Hoban, J.C., Khuder, S.A., and DeMuth, J.P. Expression measurement of many genes simultaneously by quantitative RT-PCR using standardized mixtures of competitive templates Amer. J. of Respiratory Cell and Mol. Biology, 19, 16-24, 1998.