Department of Physiology and Pharmacology

Program in Physiological Genomics


Physiological genomics laboratory (pgl)

PGL is part of the Department of Physiology and Pharmacology at the UT College of Medicine. It is also part of the Center for Hypertension and Personalized MedicinePGL utilizes an integrated approach of Genetics, Epigenetics, Genomics, Physiology of Model organisms and Bioinformatics to delineate the Genetic elements controlling blood pressure, dysfunctional actions of which may lead to the development of elevated blood pressure or hypertension.

Dr. Bina JoePrincipal Investigator:

Bina Joe, PhD

Research Interest:

The Physiological Genomics Laboratory (Joe Lab) of the Department of Physiology and Pharmacology is focused on understanding the genetic components of pathophysiological conditions of the cardiovascular, renal and autoimmune systems.  The most prominent of all complex traits investigated in the Joe laboratory is blood pressure regulation. Rat models serve as valuable alternatives to human studies for the identification and characterization of genetic factors/genes. The main strategy is to identify the disease causative genetic factor/gene based on its location on the rat genome by linkage analysis and substitution mapping and/or gene expression and protein expression profiling using whole genome systems biology approaches.  We have identified at least 16 different genomic regions that harbor quantitative trait loci (QTLs) for hypertension in rats and successfully mapped several of these regions to unparalleled resolutions of a few kilobases.  Positional cloning projects have progressed to the identification of multiple novel genes as prominent BP QTLs in rats. Methodical mechanistic studies spanning from molecular to whole-organism physiology/pathophysiology are underway to determine the functional significance of the novel BP QTLs identified.  Genetic-engineering technologies such as the application of Crispr/Cas9 targeted gene disruption for creation of 'knock-out' and 'knock-in' rat models are also strategies integrated into our research for validation of the positionally cloned causal genetic biomarkers of blood pressure regulation.  Finally, the translational significance of our work is exemplified by the validation of the positionally cloned rat genes as being associated or linked with human hypertension.  The lab has also started focusing on the gut microbiome/microbiota and its link to salt-sensitive hypertension.

Please see a complete list of Dr. Joe's publications here.

Click here to read about Joe Lab News and Announcements

Staff:

Blair Mell Joe Laboratory Manager
Physiological and Genomics Core Facility Manager
Blair Mell

Research Interest:
-The link between gut microbiota and salt-sensitive hypertension.
-Fine mapping 2 genomic regions on rat Chromosome 1 that harbor blood pressure QTLs.

Xi Cheng

Research Associate
Xi Cheng, PhD

Research Interest:
-The role of non-coding RNAs in regulating blood pressure
-The link between tumorigenesis and hypertension

Oral Presentations/Awards:
 -2015 Graduate Research Forum                       
 -2015 Midwest Graduate Research Symposium 
 -2015 Experimental Biology
 -2015 Pharmacology Research Colloquium                               
 -2015 Ohio Physiological Society
 -2016 American Heart Association Council on Hypertension
 -2016 Toledo Blade Article http://www.toledoblade.com/Medical/2016/12/05/UT-students-studying-genetic-link-to-hypertension.html
-2017 Graduate Research Forum
-2017 Outstanding Student Award (Doctor of Philosophy in Biomedical Sciences Program)
-2017 Postdoc to Faculty Award

Ji-Youn Yeo

Postdoctoral Fellow
Ji-Youn Yeo, Ph.D.

 Research Interest:

-16S metagenomics in animals and human
-Hi-C sequence mapping to detect chromatin interactions
-Diagnosis and identification of bacteria/fungi in clinical samples using MiSeq

 

Current Trainees:

fdsfsdSaroj Chakraborty
Research Interest:
-Assess the effect of variants of the gene Secreted Phosphoprotein 2 (Spp2) on blood pressure and bone mass using CRISPR/Cas9 gene-edited Knockout and knock-in rat models.
-The role of diet (Dietary fiber) on gut microbiota composition and blood pressure regulation.
-Assess the effect of chromosome conformations on blood pressure regulation
-Fine mapping genomic regions on rat Chromosome 9 for BP QTLs.

                            Oral Presentations/Awards
                           
-2017 Graduate Research Forum
                            -2017 Experimental Biology
                            -2017 Complex Trait Consortium and Rat Genome joint meeting in Memphis
 


SarahSarah Galla
Research Interest:
-The role of food emulsifiers on gut microbiota composition and blood pressure
-The mechanism behind the blood pressure elevating effect of G-protein coupled estrogen receptor 1 (GPER-1)
-The role of gut microbiota in blood pressure regulation

Oral Presentations/Awards
                           
-2017 Graduate Research Forum
                            -2017 Experimental Biology
                            -2017 Complex Trait Consortium and Rat Genome joint meeting in Memphis
                           -2017 American Physiological Society Physiological Genomics Group Annual Research Conference
                         


ZhangYoujie Zhang
Research Interest:
-Genetics of aerobic exercise capacity and its related disease risks using inbred (HCR and LCR) rats.
-Low running capacity and/or microbiota dependent risk factors for cardiovascular, metabolic, immune, and behavioral abnormalities.
-Effect of chromosome 20 (rat MHC, RT1 complex) on gut microbiota diversity and hypertension development.

                          Oral Presentations/Awards
                          
-2017 Complex Trait Consortium and Rat Genome joint meeting in Memphis

 

MSBS-MS Students:

Naveena Luke

Muhtasham Sifaat

Postdoctoral Fellows:

Xi Cheng

Ji-Youn Yeo

Volunteers:

Izuchu Efobi

Phyllis Farms


Alumni:

  • Barbara Konku
    MS/BS student, UT College of Medicine, completed in August 2017
  • Ying Nie
    Department of Physiology and Pharmacology 2011-2015 Ph.D
  • Resmi Pillai
    Department of Physiology and Pharmacology 2011- 2015 Ph.D
  • Harshal Waghulde
    Department of Physiology and Pharmacology 2012-2016 Ph.D
  • Edward Toland
    Department of Physiology, Pharmacology, Metabolism and Cardiovascular Sciences 2005-2009 Ph.D.
  • Seema Dhindaw
    Department of Physiology, Pharmacology, Metabolism and Cardiovascular Sciences 2004- MS. Completed in Aug 2007.
  • Jagannath Saikumar
    Department of Physiology, Pharmacology, Metabolism and Cardiovascular Sciences 2006- MS. Completed in May 2008.
  • Michael Bush-Arnold
    MS/BS student, UT College of Medicine. Completed in Aug 2009
  • Dipali Nemade
    MPH Research, UT College of Medicine. Completed in June 2013

Post Doctoral Fellows:

  • Suresh Koneshwaran, Cardiology fellow at UTHSC (2007)
  • Kathirvel Gopalakrishnan (2008- 2011)
  • Sivarajan Kumarasamy (2009-2014)
  • Jayakumar Thangavel (2009-2010)
  • Shakila Abdul-Majeed (2011- 2013)

Research Assistant Professors:

  • Yasser Saad, Ph.D. (2005-2006, 2007-2008)
  • Kathirvel Gopalakrishnan (2011-2014)
  • Sivarajan Kumarasamy (2014-2016)

Summer Undergraduate Students:

  • Steven Ummel, 2004 SURF program, MUO
  • Sapna Shah 2005, SURF program, MUO
  • Diona Grefer 2005, SURF program, MUO
  • Heidi Haufmann 2006, SURF program, UTHSC
  • Amal Dudar 2006, UTHSC

MD/PhD Rotation Students:

  • Joseph Lee, 2014
  • Sarah McMasters Galla, 2014

 Summer Research for Educators:

  • Jeremy Nixon, Teacher, Springfield High School; 2009 and 2012 IMAGINE 2 Program funded by Howard Hughes Medical Institute (HHMI).
  • Tamra Bacon, Teacher, Toledo Public School; 2010 IMAGINE 2 Program (HHMI)
  • Anthony Schiciano, Teacher, Ursula High School; 2011 IMAGINE 2 Program (HHMI)
  • Jeremy Nixon, Teacher, Ottawa Hills High School; 2012 IMAGINE 2 Program funded by Howard Hughes Medical Institute (HHMI).

 

Contact us:

Physiological Genomics Lab
Block Health Science Bldg., Rm. 336, MS #1008
Department of Physiology and Pharmacology
University of Toledo College of Medicine and Life Sciences
3000 Transverse Drive
Toledo, OH 43614-2598
Tel (Lab): 419-383-5174
Fax: 419-383-2871
Email: Bina.Joe@utoledo.edu

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Last Updated: 9/27/17