Flow Cytometry Core Facility

As one of the University of Toledo's core laboratories, the Flow Cytometry Core Facility provides access to instrumentation, assistance, and guidance, and offer information on current cytometric methods and applications as well as on dyes, cytometric bead arrays, multi-color panel kits, and other reagents. We welcoming UT investigators from all campuses and industrial researchers, technicians, and students from the surrounding community.

For useful information on Flow Cytometry Basics & Protocols click here

Services

The Flow Core provides high-speed cell analysis for

Immunophenotyping

Cell Tracking and Proliferation DNA Cell Cycle/Tumour Ploidy

DNA Synthesis/Degradation

Chromatin Structure

Gene Expression

Cell Membrane Potential

Surface Charge

Membrane Fusion/Runover

Ion Flux

Redox State

Oxidative Metabolism

Cellular pH Change

Cell Viability/Apoptosis/Necrosis

Intracellular Protein Staining

Total Protein

Enzyme Activity

Lipids

Sulfhydryl Groups/Glutathione

Intracellular Cytokine/Chemokine Expression

ELISA

In addition the Flow Core also offers high-speed cell sorting. This can be used to isolate/separate cell types (or vesicular bodies), rare populations, as well as specific clones post-transfection for further cell culture or analysis/experimentation—if a population can be labeled, distinguished, and gated, it can be sorted.

Samples are prepared by individual investigators who then deliver them to the Core for flow cytometric analysis or sorting. The core will not prepare cells but can provide assistance, guidance, and/or generation of publication-quality graphics and experimental design.

The Core Facility provides the following equipment:

BD FACSAria IIu High-Speed Cell Sorter

Features

Lasers

Violet- 407nm Excitation
Argon Blue- 488nm Excitation
Helium Neon Red- 633nm Excitation

12 Detectors

488nm Excitation
- FSC
- SSC- 488/10
- 530/30- 515 to 545nm
  - FITC
  - Alexa Fluor 488
  - GFP
  - CFSE
  - Annexin V
  - eYFP
- 575/26- 562 to 588nm
  - PE
  - DsRed
  - dTomato
  - RFP
- 610/20- 600 to 620nm
  - PE-Texas Red
  - Propidium Iodide (PI)
  - Alexa Fluor 610-PE
  - Nile Red
  - mCherry
- 695/40- 675 to 715nm
  - PE-Cy5
  - PerCP
  - PerCP-Cy5
  - 7AAD
- 780/60- 750 to 810nm
  - PE-Cy7
633nm Excitation
- 660/20- 650 to 670nm Emission
  - APC
  - Alexa Fluor 647
- 730/45- 707 to 752nm Emission
  - Alexa 700
  - APC-Cy5.5
  - Alexa Fluor 680
  - Alexa Fluor 700
  - DRAQ5
  - DRAQ7
- 780/60- 750 to 810nm Emission
  - APC-Cy7
  - APC-H7
407nm Excitation
- 450/40- 430 to 470nm
  - Alexa Fluor 405
  - Pacific Blue
  - Cascade Blue
  - DAPI
  - BD Horizon V450
  - Brilliant Violet 421
- 530/30- 515 to 545nm
  - Alexa Fluor 430
  - AmCyan
  - CFP
  - Qdot 525
  - BD Horizon V500
  - Cascade Yellow
  - mTurquoise

High-Speed Sort Rate up to ~25,000 events(cells)/second
Aggregate Discrimination by BD Doublet Discrimination Module
Multi-well Plate Sorter
Aerosol Management System- for use with viral samples
Temperature Controlled Sample and Sorting Receptacles

BD FACSCanto II

Features

Lasers:
- Argon Blue- 488nm Excitation
- Helium Neon Red- 633nm Excitation
Detectors
- 488nm Excitation
  - FSC
  - SSC- 488/10
  - 530/30- 515 to 545nm
    - FITC
  - 585/42- 564 to 606nm
    - PE
  - 670LP- >670nm
    - Per CP-Cy5.5
  - 780/60- 750 to 810nm
    - PE-Cy7
- 633nm Excitation
  - 660/20- 650 to 670nm
  - 780/60- 750 to 810nm

More information is available at BD Biosciences website

Services

Hours of operation, fees, and scheduling

The Flow Cytometry Core Facility will be staffed from 8:00 a.m. until 4:00 p.m., Monday through Friday.

Any inquiries or scheduling can be made by phone at 419.383.3402 or by email.

The Core Facility operates on a Fee-for-Service basis which will include data acquisition, analysis and consultation if needed.

Current Flow Rates
	Assisted	Self-Operation
UT Investigators	$60/hr	$60/hr
Outside Users	$60/hr	$60/hr

Cancellations with less than a 24-hour notice are billed for the scheduled time. Investigators are not billed for cancellations with at least 24 hour notice. Exceptions will be determined by the Core Facility.

The Core Facility is located in the Health Education Building room 233A on the University of Toledo Health Science Campus.

Located on the second floor of the Health Education Building in Room 253, the lab is directly accessible from the second floor hallway of the Pharmacy Building and Dowling Hall.

Parking is located in the front of the Health Education Building in Area 43. Alternate parking is available in Areas 44, 46 (not pictured above), and 48 with access to Health Education through the adjoining buildings or basement tunnel.

A full campus map is available for download.

Flow basics click here

Still have questions? Contact David Weaver here or 419.383.3402