Flow Cytometry Core Facility
The Flow Core provides high-speed cell analysis for
Cell Tracking and Proliferation DNA Cell Cycle/Tumour Ploidy
Cell Membrane Potential
Cellular pH Change
Intracellular Protein Staining
Intracellular Cytokine/Chemokine Expression
In addition the Flow Core also offers high-speed cell sorting. This can be used to
isolate/separate cell types (or vesicular bodies), rare populations, as well as specific
clones post-transfection for further cell culture or analysis/experimentation—if a
population can be labeled, distinguished, and gated, it can be sorted.
Samples are prepared by individual investigators who then deliver them to the Core for flow cytometric analysis or sorting. The core will not prepare cells but can provide assistance, guidance, and/or generation of publication-quality graphics and experimental design (Example).
BD Biosciences FACSCalibur (Cell Analysis Only)
More information is available at BD Biosciences website
BD FACSAria IIu High-Speed Cell Sorter
Hours of Operation, Fees, and Scheduling
The Flow Cytometry Core Facility will be staffed from 8:00 a.m. until 4:00 p.m., Monday through Friday. Any inquiries can be made by phone at 419.383.3402 or by email.
The Core Facility operates on a Fee-for-Service basis which will include data acquisition, analysis and consultation if needed.
Current Flow Rates
Cancellations with less than a 24-hour notice are billed for the scheduled time. Investigators are not billed for cancellations with at least 24 hour notice. Exceptions
will be determined by the Core Facility.
Scheduling can be done by calling 419.383.3402 or by email.
Located on the second floor of the Health Education Building in Room 253, the lab
is directly accessible from the second floor hallway of the Pharmacy Building and
Parking is located in the front of the Health Education Building in Area 43. Alternate parking is available in Areas 44, 46 (not pictured above), and 48 with access to Health Education through the adjoining buildings or basement tunnel.
A full campus map is available for download.