Biological Sciences

Faculty Research



Professor and Associate Chair

PostDoc, Fox Chase Cancer Center
Ph.D., Shanghai Institute of Biochemistry
B.Sc., Wuhan University

Office: WO 4254
Phone No: 419-530-7853

Lab Website

Research Overview:  
Over 90% of solid tumors are aneuploid. The long-term goal of our research is to understand and exploit aneuploidy in cancers. To this end, we have been investigating both subcellular structures (the centromere/kinetochore complex) and regulatory mechanisms (the mitotic checkpoint) involved in chromosome segregation. As recent results have found that aneuploid cancer cells require additional genetic or epigenetic alterations to survive and proliferate, we also aim to identify recurring modifications in aneuploid cancers and test whether they can be used for aneuploid cancer prognosis and treatment.

Even though many cancer "signature" genes have been proposed, the biological functions of some of them remain unclear. We currently focus on characterizing certain chromosomal instability (CIN) signature genes in breast cancers.  We have realized the limitations of regular 2D cell culture in studying cancers so are in different 3D cell culture systems and animal models into our research.
Current Students
Sreemita Majumdar
Lindsey Cousino
Dr. Ejaz Ahmad
Dr. Yibo Luo
Current Laboratory Grants
4/1/2018-3/30/2019  The Elsa U. Pardee Foundation, PI,
     Development of small molecules that enhance CDC20:C-MAD2 interaction to inhibit cell proliferation


5/1/2014-4/30/2019  NCI RO1CA169500, PI,
    TRIP13 AAA-ATPase overexpression in chromosomal instability and breast cancer


Publications: (*graduate student; **undergraduate student; Bold Type-lab members) 

1.  Luo, Y.B., Ahmad, E., and Liu, S.T. (2018) MAD1:  Kinetochore Receptors and Catalytic Mechanisms. Front. Cell Dev. Biol.   doi: 10.3389/fcell.2018.00051

2. *Ji, W., Luo, Y.B., Ahmad, E., and Liu, S. T. (2018) Mad1:  Direct interactions of Mitotic Arrest Deficient 1(MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling.  J BIOL Chem.  293(2):484-496. doi:10.1074/jbc.RA117.000555.Epub 2017 Nov 21.PMID:29162720

3. Liu, S.T. and *Zhang, H. (2016) The mitotic checkpoint complex (MCC): looking back and forth after 15 years.  AIMS Molecular Science, 3(4): 597-634. Review for a special issue on Cell Signaling and Signal Transduction. doi: 10.3934/molsci.2016.4.597 [link]

4. *Ji, W., *Arnst, C.A., *Tipton, A.R., Bekier, M.E. 2nd, Taylor, W. R., Yen, T.J. and Liu, S.T. (2016) OTSSP167 abrogates mitotic checkpoint through inhibiting multiple mitotic kinases. PLOS ONE. PMID: 27082996. [link] 

5. Eytan, E., *Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T.J., Liu, S.T. and Hershko, A. (2014) Disassembly of mitotic checkpoint complexes by the joint action of the AAA ATPase TRIP13 and p31comet.  Proceedings of National Academy of Sciences USA. [link]

6. *Wang, K., Sturt-Gillespie, B., Hittle, J.C., Macdonald, D., Chan, G.K., Yen, T. J. and Liu, S.T. (2014) Thyroid Hormone Receptor Interacting Protein 13 (TRIP13) AAA-ATPase is a Novel Mitotic Checkpoint Silencing Protein. Journal of Biological Chemistry. 288(49):35149-58. PMID: 24151075. [link]

7. Liu, S.T. (2014) Kinetochore: Strucuture, Function and Evolution (version 2.0), In: Encyclopedia of Life Sciences (ELS). [DOI: 10.1002/9780470015902.a0006237.pub2]. Review.

8. Ma, J., Zhang, L., *Tipton, A.R, Wu, J., Messmer-Blust, A., Philbrick, M., Qi, Y., Liu, S.T., Liu, H., Li, J. and Guo, S. (2014) Structural and Functional Analysis of the Related Transcriptional Enhancer Factor-1 (RTEF-1) and Nuclear Factor Kappa B (NF-κB) Interaction. American Journal of Physiology-Heart and Circulatory Physiology. 306(2):H233-42. PMID: 24213609 [link]

9. *Tipton, A.R., *Ji, W., Sturt-Gillespie, B., Bekier, M. E. 2nd, *Wang, K., Taylor, W. R. and Liu, S.T. (2013) Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex.   Journal of Biological Chemistry. 288(49):35149-58. PMID: 24151075. [link]

10. *Tipton, A.R., *Wang, K., **Oladimeji, P., **Sufi, S., Gu, Z. and Liu, S. T. (2012) Identification of novel mitosis regulators through data-mining with human centromere/kinetochore proteins as group queries. BMC Cell Biology. 2012, 13:15. PMID:22712476; PMCID: PMC3419070. [link] (Editor’s Picks)(Highly Accessed) (2012 June Image Highlight) (among MOST VIEWED list)

11. *Tipton, A.R., **Tipton, M., Yen, T. J. and Liu, S.T. (2011) Closed MAD2 (C-MAD2) is selectively incorporated into the mitotic checkpoint complex (MCC). Cell Cycle. 10(21): 3740-50. PMID: 22037211. [link]

12. *Tipton, A.R., *Wang, K., **Link, L., Bellizzi, J.J., Huang, H. Yen, T. J. and Liu, S.T. (2011) BUBR1 and closed MAD2 (C-MAD2) interact directly to assemble a functional Mitotic Checkpoint Complex (MCC). Journal of Biological Chemistry. 286(24):21173-9. PMID: 21525009; PMCID: PMC3122179. [link]

Last Updated: 3/2/22