Biological Sciences

Faculty Research

Scott Leisner


Scott Leisner

Professor
Ph.D., Purdue University, 1989
B.S., University of Wisconsin

Office: WO 1235N
Phone No: 419.530.2066 
Email: scott.leisner@utoledo.edu

 

 

Research

As global populations increase, it is important to ask the question, “How are we going to feed all of these people?” Like humans, plants are exposed to a variety of stress. The more effectively we can help plants deal with stress, the greater crop productivity would increase and that would help to feed the growing number of hungry people. Our research examines how we can help plants to better deal with stress. Currently, we are studying two different kinds of stress that plants are exposed to, diseases and heavy metal pollutants. 

The disease that we are studying is caused by a pathogen called Cauliflower mosaic virus (CaMV). CaMV infects a wide variety of plants within the mustard family including, turnips, brussels sprouts, cauliflower, broccoli, and cabbage. The virus is transmitted by insects from infected to healthy plants. Once the virus is delivered into the inside of a healthy plant cell, the virus particle opens up, releasing the viral genomic DNA into the plant cell nucleus. Inside the plant cell nucleus the viral genomic DNA is transcribed to make viral RNAs. The viral RNAs are then used to make both new viral DNAs and proteins, which are assembled into new virus particles, to repeat the cycle. Just as with humans making a large number of complex products such as cars, the production and assembly of virus components does not occur randomly within the cell, but in specialized “factories.” Viral factories are where most CaMV processes occur. In our laboratory, we are studying how these factories work. We are examining the major constituent of viral factories, a protein called P6, and are determining how its interactions with viral and host proteins allow it to make these factories. Our most recent data indicate that mutations affecting the organization of factories drastically delays viral infection and shows what a good target this protein is for inhibiting viral infection. Such research will lead us to understand not just CaMV, but other types of viruses so we can stop them.

The heavy metal pollutant that we are studying is copper (Cu), an essential element for all organisms. However, if Cu levels get too high, they can cause toxicity effects, which harm the organism, in our case, the plant. We and others, have discovered that the element silicon (Si) helps plants to better deal with a variety of stress such as Cu toxicity. This is very interesting because Si confers many beneficial effects on plants and yet, we have no idea how this element works. Not only does Si help the plant to better deal with stress but stress also induces the uptake of Si into plant leaves. This means that Si uptake is regulated and that there must be transporter that brings Si into plants. We have recently discovered a Si transporter and are currently working to understand how it functions and is regulated. Such an understanding will permit us to better help plants deal with stress and thereby, improve crop productivity.

Current Graduate Students

Roberto Alers-Velasquez

Current Laboratory Grants

USDA-ARS
Specific Cooperative Agreement: Analysis of mechanisms involved in induction of abiotic or biotic stress tolerance in horticulture crops through nutrition or temperature.

Publications

Last Updated: 3/2/22