University of Toledo Miniscope Imaging Core
The University of Toledo Miniscope Imaging Core is established to meet the increasing need for conducting high-resolution calcium imaging within the brains of freely behaving rodents among researchers and scientists at the University of Toledo. This innovative neurotechnology involves the utilization of Genetically Expressed Calcium Indicators (GECI) at specific brain coordinates of mice, rats or voles. Implanting a Gradient Refractive Index (GRIN) lens over the target brain region allows for the collection and transmission of fluorescent data, indicating neuronal activity, through optical filters onto a CMOS sensor. This enables investigators to monitor real-time activity of GECI-expressing neurons as animals engage in behavioral tasks using a head-mounted miniature microscope, or miniscope, weighing less than 2 grams, granting animals the freedom of movement. The Core is designed with the vision to train students, postdocs, and research staff at the University of Toledo in performing implantation surgeries and cultivating high-quality researchers proficient in harnessing this cutting-edge neurotechnology.
About Us
Firing activity of neurons in the anterior cingulate cortex of an awake, behaving adult mouse.
At The University of Toledo Miniscope Imaging Core, our primary mission is to empower UToledo faculty and staff with cutting-edge neurotechnology. Specializing in the emerging field of miniscope imaging, we offer unparalleled access to a revolutionary method enabling the simultaneous recording of neuronal activity from hundreds of neurons in the brain of an awake, behaving rodent. Committed to advancing neuroscientific research, our core provides a dynamic platform for exploring brain function in real-world conditions.
Our team of experts, driven by a passion for innovation, collaborates with researchers, fostering an environment that encourages breakthroughs in understanding neural circuits. Join us at the forefront of neuroscientific exploration as we unlock the mysteries of the brain through the transformative capabilities of miniscope imaging.
Learn more about Core Research Facilities at The University of Toledo.
Meet our Team
Under the guidance of Director James Burkett, Ph.D., our team is dedicated to unraveling the mysteries of neural circuits. The core goes beyond being a facility; it is a collaborative hub fostering interdisciplinary research and innovation.
Join us in exploring the exciting realm of miniscope imaging and be part of a community where curiosity fuels groundbreaking discoveries in the field of neuroscience. Welcome to the future of neuroscientific inquiry.
Directors:
Equipment and Facilities
The University of Miniscope Imaging Core is equipped with state-of-the-art tools and technologies to facilitate groundbreaking neuroscientific research. Our facilities include:
- Inscopix nVoke 2.0 (Optogenetics and Calcium Imaging)
- Advanced capabilities for optogenetics and calcium imaging, unlocking unprecedented insights into neural activity.
- Inscopix nVue (Dual Channel Recording)
- Dual-channel recording capability, providing enhanced flexibility in data acquisition.
- Inscopix nVision (Synchronized Imaging and Behavior)
- Cutting-edge technology ensuring synchronized imaging with behavioral observations, enabling comprehensive analysis.
- Neurostar® Robotic Stereotaxic System
- Precise and automated stereotaxic system for accurate positioning in neurosurgical procedures.
- Inscopix IDEAS Platform
- A comprehensive platform supporting experimental design, data acquisition, and analysis.
- Inscopix Data Analysis Software
- Powerful software tools for efficient and insightful data analysis.
Rates and Services
Explore our range of services and associated rates tailored to meet the diverse needs of researchers:
Consultation Services
Experiment Design: $100 per hour (UToledo) / $125 per hour (Other academic institutions) / $150 per hour (Non-academic institutions)
Staff Scientist Support
Consultation: $100 per hour (UToledo) / $125 per hour (Other academic institutions) / $150 per hour (Non-academic institutions)
Surgical Services
Surgical Training: $1000 per 10 hours
Surgery performed by Staff Scientist: $400 per animal (UToledo) / $500 per animal
(Other academic institutions) / $600 per animal (Non-academic institutions)
Data Recording
nVoke or nVue: $240 per 4 hours (plus staff time as needed)
nVision (additional): $80 per 4 hours
Data Analysis Services
Training: $1000 per 10 hours
Performed by Staff Scientist: Hourly rate based on consultation
Cloud Data Storage
Fee-based on consultation
*Consumables, including GRIN lens, viruses, and animals, will be billed to the Principal
Investigator (PI).
For any specific inquiries or to schedule services, please feel free to contact us.