University of Toledo

Mandatory for Anyone Working in the Vivarium

This online only training offers a foundational overview of DLAR policies and procedures, including the proper use of personal protective equipment (PPE), security protocols, facility access, operational functions, DLAR-specific processes, and more.

Required Prerequisite:

1. IACUC required CITI training

How to Complete:

1. Log in to Test Bank.
2. Review DLAR Orientation (1671) PowerPoint presentation
3. Complete and pass accompany test
4. Be sure to complete and submit your Occupational Health Questionnaire. Do NOT send completed forms to DLAR.

*Annual refresher required.

Mandatory for Anyone Using Animals in Research

This hands-on animal training is species specific and includes a vivarium and/or room tour, basic techniques for animal handling and restraint, subcutaneous injection, and methods of euthanasia.

You must complete all handling and restraint courses for every species you will be working with.

For rabbit handling and restraint training, contact Jamie Harris at Jamie.Harris@utoledo.edu

Required Prerequisites:

1. IACUC required CITI training
2. DLAR Orientation (1671) in Test Bank
3. Handling and restraint in the mouse video
4. Substance administration video

How to Register:

1. Log in to Test Bank.
2. Select Class Calendar under Testing/Training/Tracking at the top.
3. In the training type drop down list, select Dept of Lab Animal Resources
4. Using the calendar, select a day/time for the class you need
5. Click on Register

Advanced Techniques Based on What's in Your Protocol

This hands-on animal training is species specific and focuses on advanced technical skills using mice. Topics covered include ear tagging/punching, intraperitoneal injections, facial blood collection, oral gavage, tail vein injections, and more.

You must read your protocol to determine which procedures you need training on. Once you sign up for training, DLAR will contact you to establish the specific training you need. 

For rabbit technical procedures training, contact Jamie Harris at Jamie.Harris@utoledo.edu 

Required Prerequisites:

1. IACUC required CITI training
2. DLAR Orientation (1671) in Test Bank
3. DLAR Rodent Handling and Restraint

How to Register:

1. Log in to Test Bank.
2. Under Testing/Training/Tracking select Class Calendar
3. Select Dept of Lab Animal Resources in the training type drop down list
4. Pick a day/time for the class you need
5. Click on Register

Supplemental Materials 

1. JOVE Video on Lab Animal Research
2. Mouse and Rat Oral Gavage Training Video
3. Mouse Intraperitoneal Injections
4. Rat Intraperitoneal Injections
5. Blood collection guidelines from the IACUC
6. Tail Vein Injections in Mice

Mandatory for Anyone Using Isoflurane Gas Anesthesia for Procedures or Surgeries Under an I.A.C.U.C. Approved Protocol

This hands-on animal training covers anesthetic equipment setup, induction and maintenance of anesthesia in rodents, shaving and surgical site preparation, monitoring during anesthesia, and post-anesthetic supportive care.

For rabbit isoflurane anesthesia training, contact Jamie Harris at Jamie.Harris@utoledo.edu 

Required Prerequisites:

1. IACUC required CITI training
2. DLAR Orientation (1671) in Test Bank
3. DLAR Animal Handling and Restraint
4. Review the JOVE article Anesthesia Induction and Maintenance

How to Register:

1. Log in to Test Bank.
2. Under Testing/Training/Tracking select Class Calendar
3. Select Dept of Lab Animal Resources in the training type drop down list
4. Pick a day/time for the class you need
5. Click on Register

Mandatory for Anyone Performing Surgery on Rodents

This hands-on animal training focuses on aseptic technique, including creating and maintaining a sterile field, proper sterile gloving, instrument handling, making incisions and performing closures, as well as reviewing post-operative care and monitoring procedures.

Required Prerequisites:

1. Complete required DLAR training
   • IACUC required CITI training
   • DLAR Orientation (1671) in Test Bank
   • DLAR Rodent Handling and Restraint
   • DLAR Isoflurane Anesthesia
2. Watch these videos
   • Rodent Survival Surgery
   • Rodent skin prep
   • Putting on sterile gloves
   • Instrument handling video
   • Suturing video (only required for those using suture to close incisions)
3. Review the following information
   • Surgery Packet
   • Surgery Room Set-up
   • Surgery Steps Checklist
   • UCSF Rodent Surgery Anesthesia Record

How to Register:

1. Log in to Test Bank.
2. Under Testing/Training/Tracking select Class Calendar
3. Select Dept of Lab Animal Resources in the training type drop down list
4. Pick a day/time for the class you need
5. Click on Register

Coming Soon - Check Back for Updates

This online only training provides an overview of pre-surgical preparation, from anesthesia induction to sterile skin preparation. It also covers surgical instrument packing and preparation, post-operative monitoring and documentation, incision grading, and the distinction between sterile and non-sterile techniques.

Required Prerequisites:

• IACUC required CITI training
• DLAR Orientation (1671) in Test Bank
• DLAR Rodent Handling and Restraint
• DLAR Isoflurane Anesthesia

How to Complete:

1. Log in to Test Bank.
2. Review DLAR Surgical Assisting & Post Operative Monitoring PowerPoint presentation
3. Complete and pass accompany test

Mandatory 2 PART Training for Using Biological or Chemical Hazards.

Part 1 consists of an online module and quiz, while Part 2 is an in-person review. Together, they provide a comprehensive overview of the policies and procedures for safely handling hazardous materials in animal research.

Required Prerequisites:

1. DLAR Orientation (1671) in Test Bank
2. DLAR Animal Handling and Restraint

How to Complete and Register:

 *Annual refresher required for Part 1 only

Mandatory for Anyone Approved to Breed Rodents on Their Protocol

This online only presentation and quiz covers the fundamentals of mouse and rat reproduction, along with key concepts in breeding colony management.

Additional in-person training available upon request.

Required Prerequisites:

1. DLAR Orientation (1671) in Test Bank
2. DLAR Animal Handling and Restraint

How to Complete:

1. Log in to Test Bank.
2. Review DLAR Rodent Breeding (2751) PowerPoint presentation
3. Complete and pass accompany test

Supplemental Materials:

1. Breeding Packet of helpful information: Breeding Packet
2. Example of an Excel spreadsheet used for managing a breeding colony.

Mandatory for Anyone Performing In-Vivo Tumor Experiments on Their Protocol

This online only presentation and quiz provide an overview of the requirements outlined in the IACUC Rodent Tumor Guidelines.

Additional in-person training available upon request.

Required Prerequisites:

1. DLAR Orientation (1671) in Test Bank
2. DLAR Animal Handling and Restraint

How to Complete:

1. Log in to Test Bank.
2. Review DLAR Rodent Tumor Monitoring (2750) PowerPoint presentation
3. Complete and pass accompany test