University of Toledo

Training Requirements and Classes

Getting Started

  1. You must be on an approved IACUC protocol.
    • If you are not on a protocol yet, your Principle Investigator (PI) must add you to the protocol. 
    • This can be done by logging into IRB Manager and completing a personnel amendment.
  2. You must have completed all of the required CITI training per the IACUC training requirements guideline.
  3. With your PI, determine what DLAR training is required for your lab. Everyone is required to take DLAR Orientation and Rodent Handling and Restraint. Sign up by logging into Test Bank.
    • In the upper left click on Testing/Training/Tracking.
    • Then click on Class Calendar.  Make sure Dept of Lab Animal Resources is listed towards the top under 'Select a Class Name'.  If not, click the drop down box and select Dept of Lab Animal Resources.

available dlar classes

dlar orientation*

mandatory online training

This session covers DLAR policies and procedures, including the difference between Specific Pathogen Free (SPF), Conventional and Containment housing, traffic flow, facility security, access, etc.     

Prerequisities PRIOR to taking this training

Training

  1. Log in to Test Bank.
  2. Search for DLAR Orientation (1671).
  3. Select, TAKE SELECTED TEST.  
  4. Once you have passed the DLAR Orientation test you must then submit your Occupational Health Questionnaire.
  5.  
rodent handling & restraint*

mandatory for anyone using rodents in research

This is a hands-on lab that covers basic rodent handling, subcutaneous injections, and euthanasia. If you’re going to work with both rats and mice, you must sign up for both. 

Prerequisities PRIOR to taking this training

  1. IACUC required CITI training
  2. DLAR Orientation in Test Bank (must pass the test)
  3. Handling and restraint in the mouse video
  4. Substance administration video

Sign Up Here

technical procedures

advanced techniques based on what's in your protocol

These are species specific wet labs. We cover advanced blood collection and injection procedures such as submandibular bleeding, intraperitoneal injections, oral gavage, tail vein injections, tail nick, etc.  You must read your protocol to determine which procedures you need training on, since there are many.  Once you sign up for the training time, DLAR will contact you to establish the specific training you need. 

Prerequisities PRIOR to taking this training

  1. IACUC required CITI training
  2. DLAR Orientation in Test Bank (must pass the test)
  3. Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)

Helpful materials to review and reference

  1. JOVE Video on Lab Animal Research
  2. Mouse and Rat Oral Gavage Training Video
  3. Mouse Intraperitoneal Injections
  4. Rat Intraperitoneal Injections
  5. Blood collection guidelines from the IACUC
  6. Tail Vein Injections in Mice

Sign Up Here

isofurane anesthesia

mandatory for anyone using gas anesthesia in rodents

This session is required for anyone performing isoflurane anesthesia for procedures or surgery on an IACUC protocol. We will cover the setup of the equipment, anesthesia induction and maintenance, monitoring, and post-anesthesia supportive care.

Prerequisities PRIOR to taking this training

  1. IACUC required CITI training
  2. DLAR Orientation in Test Bank (must pass the test)
  3. Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)
  4. Review the JOVE article Anesthesia Induction and Maintenance

Sign Up Here

aseptic technique & surgery

mandatory for anyone performing surgery on animals

This session 3-hour session covers aseptic technique, instrument preparation, incisions, closure, pre- and post-op care. Only attend if you will be performing surgery.  

Prerequisities PRIOR to taking this training - If you do not complete the below prerequisites, you CANNOT attend this lab.

  1. Complete required DLAR training
    • IACUC required CITI training
    • DLAR Orientation in Test Bank (must pass the test)
    • Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)
    • Isoflurane Anesthesia
  2. Watch the videos
  3. Review the information

Sign Up Here

hazard training

mandatory 2 PART training for anyone approved to use biological or chemical hazards.

The on-line training and quiz is required for anyone using Biological or Chemical Hazards in the vivarium.  It’s a comprehensive review on how to safely work with hazards in animals within DLAR and understanding the approved hazardous signage from EHRS.   

Prerequisities prior to taking this training

  1. DLAR Orientation in Test Bank
  2. Rodent Handling and Restraint

Training

Part 1 - Online Training & Test

  1. Log in to Test Bank.
  2. Search for Hazard Training (1686)
    (pay attention you will have a quiz on this PowerPoint).
  3. Select, TAKE SELECTED TEST

Part 2 - In Person Training in DLAR

  1. Log in to Test Bank.
  2. Search for Hazard Training (79).
  3. Select the next available in person class that you can attend. 
Rodent Breeding

mandatory for anyone approved to breed rodents on their protocol

This on-line training and quiz reviews the fundamentals of mouse and rat reproduction and breeding colony management.

Training

  1. Log in to Test Bank.
  2. Search for Rodent Breeding (pay attention--you will have a quiz on this PowerPoint).
  3. Select, TAKE SELECTED TEST

Helpful materials to review and reference

  1. Breeding Packet of helpful information: Breeding Packet
  2. Example of an Excel spreadsheet used for managing a breeding colony.
rodent tumor guidelines

mandatory for anyone performing in vivo tumor experiments on their protocol

This on-line training and quiz reviews the requirements set forth by the IACUC Rodent Tumor Guideline.

  1. Log in to Test Bank.
  2. Search for Rodent Tumor Monitoring Guideline (pay attention--you will have a quiz on this PowerPoint).
  3. Select, TAKE SELECTED TEST.

Security Access to DLAR

* Your security access to DLAR activates after you've COMPLETED THE REQUIRED TRAINING!

  • Required Training = DLAR Orientation + Rodent Handling and Restraint.
  • Health Science Campus (Health Education Building) DLAR access requires an Access Card (aka "Proxy Card") in addition to your UToledo ID.  Follow the steps outlined in the DLAR Orientation to get a Proxy Card. Proxy cards can be obtained at the HSC Security Office in the basement of Mulford Library.
  • Main Campus access utilizes your UToledo Rocket ID badge only (no Proxy Card required).  

Contact the DLAR office at 419.383.4310 for any questions.

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Last Updated: 9/29/23