University of Toledo

Training Requirements and Classes

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Getting started

  1. You must be on an approved IACUC protocol.
    • If you are not on a protocol yet, your Principle Investigator (PI) must add you to the protocol. 
    • This can be done by logging into IRB Manager and completing a personnel amendment.
  2. You must have completed all of the required CITI training per the IACUC training requirements policy.

 3. With your PI, determine what DLAR training is required for your lab. Everyone is required to take DLAR Orientation and Rodent Handling and Restraint. Sign up by logging into Test Bank.

    • In the upper left click on Testing/Training/Tracking.
    • Then click on Class Calendar.  Make sure Dept of Lab Animal Resources is listed towards the top under 'Select a Class Name'.  If not, click the drop down box and select Dept of Lab Animal Resources.

 

Available DLAR Classes

1)  DLAR ORIENTATION* (Mandatory On-Line Training):

This session covers DLAR policies and procedures, including the difference between Specific Pathogen Free "SPF", Conventional and Containment housing, traffic flow, facility security, access, etc.            

    1. Log in to Test Bank.
    2. Search for DLAR Orientation (1671).
    3. Select, TAKE SELECTED TEST.  
    4. Once you have completed DLAR Orientation you must then submit your Occupational Health Questionnaire.

Prerequisites prior to taking this training: 

    1. IACUC required CITI training

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 2)  Rodent Handling and Restraint* (Mandatory for Anyone Using Rodents in Research): 

These are species-specific 2-hour hands-on labs. The length of session depends on experience and number of trainees per session. This hands-on lab covers basic rodent handling, injections, and euthanasia. If you’re going to work with both rats and mice, sign up for both. 

 Prerequisites prior to taking this training: 

    1. DLAR Orientation
    2. Handling and restraint in the mouse video
    3. Substance administration video

 Sign up here:   

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3)  TECHNICAL PROCEDURES (Advanced Techniques Based on What’s in Your Protocol):

These are species specific 2-hour wet labs. We cover advanced blood collection and injection procedures such as submandibular bleeding, intraperitoneal injections "IP", oral gavage, tail vein injections, tail nick, etc.  You must read your protocol to determine which procedures you need training on, since there are many.  Once you sign up for the training time, DLAR will contact you to establish the specific training you need. 

Prerequisites prior to taking this training: 

    1. DLAR Orientation
    2. Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)

Helpful materials to review and reference:

    1. https://www.jove.com/science-education- library/23/lab-animal-research
    2. Mouse and Rat Oral Gavage Training Video
    3. Mouse Intraperitoneal Injections
    4. Rat Intraperitoneal Injections
    5. Blood collection guidelines from the IACUC
    6. Tail Vein Injections in Mice

Sign up here: 

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 4)  ISOFLURANE ANESTHESIA (Mandatory for Anyone Using Gas Anesthesia in Rodents):

This 1-hour session is required for anyone performing isoflurane anesthesia for procedures or surgery on an IACUC protocol. We will cover the setup of the equipment, anesthesia induction and maintenance, monitoring, and post-anesthesia supportive care.

 Prerequisites prior to taking this training: 

    1. DLAR Orientation
    2. Rodent Handling and Restraint
    3. Review the JOVE article Anesthesia Induction and Maintenance

Sign up here:

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5)  ASEPTIC TECHNIQUE & SURGERY (Mandatory for Anyone Performing Surgeries on Animals):

This session 3-hour session covers aseptic technique, instrument preparation, incisions, closure, pre- and post-op care. Only attend if you will be performing surgery.  

 Prerequisites prior to taking this training: 

******If you do not complete the below prerequisites, you cannot attend this lab. ******

    1. Complete required DLAR training
      • DLAR Orientation
      • Rodent Handling and Restraint
      • Technical Procedures
      • Isoflurane Anesthesia
    2. Watch the videos:

3. Review the information:

 Sign up here: 

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 6) HAZARD TRAINING (Mandatory 2 PART Training for Anyone Approved to Use Hazards--Biological or Chemical):

The on-line training and quiz is required for anyone using Biological or Chemical Hazards in the vivarium.  It’s a comprehensive review on how to safely work with hazards in animals within DLAR and understanding the approved hazardous signage from EHRS.   

Prerequisites prior to taking this training: 

    • Orientation
    • Rodent Handling and Restraint 

Part 1 (online training and test):

    1. Log in to Test Bank.
    2. Search for Hazard Training (1686) (pay attention you will have a quiz on this PowerPoint).
    3. Select, TAKE SELECTED TEST.  

Part 2 (in person training in DLAR):

    1. Log in to Test Bank.
    2. Search for Hazard Training (79).
    3. Select the next available in person class that you can attend.  

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7)  RODENT BREEDING (Mandatory for anyone approved to breed rodents on their protocol)

This on-line training and quiz reviews the fundamentals of mouse and rat reproduction and breeding colony management.

    1. Log in to Test Bank.
    2. Search for Rodent Breeding (pay attention you will have a quiz on this PowerPoint).
    3. Select, TAKE SELECTED TEST

 

Helpful materials to review and reference: 

    1. Breeding Packet of helpful information: Breeding Packet
    2. Example of an excel spreadsheet used for managing a breeding colony 

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8)  RODENT TUMOR GUIDELINES (Mandatory for anyone performing in vivo tumor experiments on their protocol):

This on-line training and quiz reviews the requirements set forth by the IACUC Rodent Tumor Guideline.

    1. Log in to Test Bank.
    2. Search for Rodent Tumor Monitoring Guideline (pay attention you will have a quiz on this PowerPoint).
    3. Select, TAKE SELECTED TEST.

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*YOUR SECURITY ACCESS TO DLAR ACTIVATES AFTER YOU'VE COMPLETED THE REQUIRED TRAINING!

  • *Required training includes DLAR Orientation and Rodent Handling and Restraint.
  • Health Science Campus (Health Education Building) DLAR access requires an Access Card (aka "Proxy Card") in addition to your UT ID.  Follow the steps outlined in the DLAR Orientation to get a Proxy Card.
  • Main Campus access utilizes your UT ID badge only (no Proxy Card required).  

 Contact the DLAR office at 419-383-4310 for any questions.  

Last Updated: 11/24/21