University of Toledo

mandatory online training

This session covers DLAR policies and procedures, including the difference between Specific Pathogen Free (SPF), Conventional and Containment housing, traffic flow, facility security, access, etc.     

Prerequisities PRIOR to taking this training

• IACUC required CITI training

Training

1. Log in to Test Bank.
2. Search for DLAR Orientation (1671).
3. Select, TAKE SELECTED TEST.  
4. Once you have passed the DLAR Orientation test you must then submit your Occupational Health Questionnaire.
5.  

mandatory for anyone using rodents in research

This is a hands-on lab that covers basic rodent handling, subcutaneous injections, and euthanasia. If you’re going to work with both rats and mice, you must sign up for both. 

Prerequisities PRIOR to taking this training

1. IACUC required CITI training
2. DLAR Orientation in Test Bank (must pass the test)
3. Handling and restraint in the mouse video
4. Substance administration video

Sign Up Here

• Mouse Lab Sign Up  
• Rat Lab Sign Up

advanced techniques based on what's in your protocol

These are species specific wet labs. We cover advanced blood collection and injection procedures such as submandibular bleeding, intraperitoneal injections, oral gavage, tail vein injections, tail nick, etc.  You must read your protocol to determine which procedures you need training on, since there are many.  Once you sign up for the training time, DLAR will contact you to establish the specific training you need. 

Prerequisities PRIOR to taking this training

1. IACUC required CITI training
2. DLAR Orientation in Test Bank (must pass the test)
3. Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)

Helpful materials to review and reference

1. JOVE Video on Lab Animal Research
2. Mouse and Rat Oral Gavage Training Video
3. Mouse Intraperitoneal Injections
4. Rat Intraperitoneal Injections
5. Blood collection guidelines from the IACUC
6. Tail Vein Injections in Mice

Sign Up Here

• Mouse Technical Procedures Sign Up
• Rat Technical Procedures Sign Up

mandatory for anyone using gas anesthesia in rodents

This session is required for anyone performing isoflurane anesthesia for procedures or surgery on an IACUC protocol. We will cover the setup of the equipment, anesthesia induction and maintenance, monitoring, and post-anesthesia supportive care.

Prerequisities PRIOR to taking this training

1. IACUC required CITI training
2. DLAR Orientation in Test Bank (must pass the test)
3. Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)
4. Review the JOVE article Anesthesia Induction and Maintenance

Sign Up Here

• Isofurane Anesthesia Sign Up

mandatory for anyone performing surgery on animals

This session 3-hour session covers aseptic technique, instrument preparation, incisions, closure, pre- and post-op care. Only attend if you will be performing surgery.  

Prerequisities PRIOR to taking this training - If you do not complete the below prerequisites, you CANNOT attend this lab.

1. Complete required DLAR training
   • IACUC required CITI training
   • DLAR Orientation in Test Bank (must pass the test)
   • Rodent Handling and Restraint (You must be proficient in handling and restraint prior to attending this lab.)
   • Isoflurane Anesthesia
2. Watch the videos
   • Rodent Survival Surgery
   • Rodent skin prep
   • Putting on sterile gloves
   • Instrument handling video
   • Suturing video (only required for those using suture to close incisions)
3. Review the information
   • Surgery Packet
   • Surgery Room Set-up
   • Surgery Steps Checklist
   • UCSF Rodent Surgery Anesthesia Record

Sign Up Here

• Aseptic Technique & Surgery Sign Up

mandatory 2 PART training for anyone approved to use biological or chemical hazards.

The on-line training and quiz is required for anyone using Biological or Chemical Hazards in the vivarium.  It’s a comprehensive review on how to safely work with hazards in animals within DLAR and understanding the approved hazardous signage from EHRS.   

Prerequisities prior to taking this training

1. DLAR Orientation in Test Bank
2. Rodent Handling and Restraint

Training

mandatory for anyone approved to breed rodents on their protocol

This on-line training and quiz reviews the fundamentals of mouse and rat reproduction and breeding colony management.

Training

1. Log in to Test Bank.
2. Search for Rodent Breeding (pay attention--you will have a quiz on this PowerPoint).
3. Select, TAKE SELECTED TEST

Helpful materials to review and reference

1. Breeding Packet of helpful information: Breeding Packet
2. Example of an Excel spreadsheet used for managing a breeding colony.

mandatory for anyone performing in vivo tumor experiments on their protocol

This on-line training and quiz reviews the requirements set forth by the IACUC Rodent Tumor Guideline.

1. Log in to Test Bank.
2. Search for Rodent Tumor Monitoring Guideline (pay attention--you will have a quiz on this PowerPoint).
3. Select, TAKE SELECTED TEST.