University of Toledo Integrated Core Facilities (ICF)

Andrea L. Kalinoski, Ph.D.

Andrea Kalinoski, Ph.D. Associate Professor
Technical Director Integrated Core Facilities
Office: 419.383.4205


B.A., Biology, University of Toledo, Toledo, Ohio 1994
Ph.D., Medical Sciences, Medical College of Ohio, Toledo, Ohio, 2003 


  • University of Toledo Health Science Campus, Department of Surgery, Toledo, Ohio 2003-2009 


  • 1994- 1998: Medical College of Ohio, Department of Surgery, Research Associate, Toledo, Ohio 
  • 2003- 2009: Post-Doc Fellow, Department of Surgery, Toledo Ohio, Toledo Ohio 
  • 2006- 2013: Technical Director, Advanced Microscopy & Imaging Center, Toledo Ohio, Toledo Ohio 
  • 2009- 2014: Assistant Professor, University of Toledo, Department of Surgery, Toledo, Ohio 
  • 2013- Present: Technical Director, UT Integrated Core Facilities (Advanced Microscopy & Imaging Center, Flow Cytometry and Histology), University of Toledo, College of Medicine and Life Sciences
  • 2015- Present: Associate Professor with Tenure, University of Toledo, Department of Surgery, Toledo, Ohio  


  • SPARC OT2OD023859 Howard (PI) 10/15/2016-09/30/2019 NIH
    Role Kalinoski (CO-I) Anatomical-Functional Mapping of Enteric Neural Circuits
  • University Research Award and Fellowship Vestal (PI) 6/01/2018-05/31/2019 
    Role Kalinoski (CO-I) GBP-1 in Glioblastoma
  • R01CA169500 Liu (PI) 05/01/2014-04/30/2018 NIH
    Role Kalinoski (CO-I) Trip12 AAA-ATPase overexpression in Chromosomal Instability and Breast Cancer


  1. Understanding the molecular signaling mechanisms within the vascular wall that results in the injury response and formation of neointimal hyperplasia.
  2. Understanding the signaling mechanisms within the vascular wall that result in fibroelastic changes and lead to arterial stiffness and/or decreased vascular tone. 


The University of Toledo Integrated Core Facilities acquire, maintain and support state-of-the-art equipment thatenables cutting-edge research to be performed by helping researchers economically and efficiently take advantage of innovative technology and collaborate with experts in the field.



  1. Allison DC, Nestor AL.  (1999) Evidence for a relatively random array of human chromosomes on the mitotic ring.  J Cell Bio 145: 1-14. 
  2. Assadnia S, Rapp JP, Nestor AL, Cerilli GJ, Webb TH, Kligman M, Allison DC.  (1999) Strain differences in neointimal hyperplasia in the rat. Circ Res 84: 1252-1257. 
  3. *Isaka T, *Nestor AL, Takada T, Allison DC.  (2003) Chromosomal variations within aneuploid cancer lines.  J Histochem Cytochem 51(10): 343-1353.  (*equal authorship)
  4. Nestor AL, Cicila GT, Karol SE, Langenderfer KM, Hollopeter SL, Allison DC.  (2006) Linkage analysis of neointimal hyperplasia and vascular wall changes following balloon angioplasty.  Physiol Genomics 25: 286-93. 
  5. Nestor AL, Hollopeter SL, Matsui SI, Allison DC. (2007) A Model for Genetic Complementation Controlling the Chromosomal Abnormalities and Loss of Heterozygosity Formation in Cancer.  Cytogenet Genome Res 116:235-247. 
  6. Nestor Kalinoski AL, Ramdath RS, Langenderfer KM, Sikanderkhel S, DeRaedt S, Welch M, Park JL, Pringle T, Joe B, Cicila GT and Allison DC.  (2010) Neointimal Hyperplasia and Vasoreactivity are Controlled by Genetic Elements on Rat Chromosome 3. Hypertension 55(2):555-561. 
  7. Stewart K, Olorunto W, Fink B, Zelenock, G, Allison DC, Nestor Kalinoski AL.  (2010) Resuscitation with Ketones Improves Survival Over Lactated Ringers in a Rat Shock Model.  JACS 211(3):S52-3, 2010.
  8. Bazeley PS, Nestor Kalinoski AL, Ways JA, Liu ST, Ramdath RS, Matsui SI and Allison, DC.  (2011) A Model for Random Genetic Damage Directing the Selection of Diploid or Aneuploid Tumors.  Cell Proliferation 44: 212-223.  PMID: 21535262
  9. Wyse MM, Lei J, Nestor-Kalinoski AL, and Eisenmann KM.  (2012)  Dia-interacting protein (DIP) imposes migratory plasticity in mDia2-dependent breast cancer cells in three-dimensional matrices.  PLOS One 7(9): e45085.  PMID: 23024796.
  10. Gopalakrishnan K, Kumarasamy S, Abdual-Majeed S, Kalinoski A, Morgan EE, Gohara AF, Nauli SM, Filipiak WE, Saunders TL and Joe B. (2012) Validation of a Blood Pressure Quantitative Trait Gene Using a Targeted Disruption Rat Model.  Proc Natl Acad Sci U S A. 109(50):20555-9.  PMID: 23185005.
  11. Wagner DE, Eisenmann K , Nestor-Kalinoski AL, Bhaduri, SB. (2012) A Novel Microwave Assisted Solution Combustion Synthesis (MASCS) to Produce Europium Doped Calcium Phosphate Nanowhiskers for Multifunctional Bioimaging Applications. Acta Biomaterialia. (9):8422-32.  PMID:  23764803.
  12. Nesamony JKalra AMajrad MSBoddu SHJung RWilliams FESchnapp AMNauli SMKalinoski AL. (2013)  Development and Characterization of Nanostructured Mists with Potential for Actively Targeting Poorly Water-Soluble Compounds into the Lungs.   Pharm Res.10:2625-39.  PMID:  23722411.
  13. Weaver, DA, Nestor-Kalinoski AL, Craig K, Gorris, M, Parikh T, Mabry H, Allison DC.  (2014) Correction of Sampling Errors Finds Increased mRNA Levels Possibly Compensating for Cancer Haplo-Insufficiency.  Genes Chromosomes Cancer  53(2):194-210   PMID:  24327546.
  14. Pettee KM, Dvorak KM, Nestor-Kalinoski ALand Kathryn M Eisenmann KM.  (2014)  An mDia2/ROCK Signaling Axis Regulates Invasive Egress from Epithelial Ovarian Cancer Spheroids.  PLoS One. 2014; 9(2): e90371.  PMID: 24587343.
  15. Morgan EE, Casabianca AB, Khouri S, and Nestor-Kalinoski, AL. (2014) In vivo Assessment of Arterial Stiffness in the Isoflurane Anesthetized Spontaneously Hypertensive Rat.  Cardiovascular Ultrasound, 12:37. PMID:  25227282
  16. Arden JD, Lavik KI, Rubinic KA, Chiaia N, Khuder SA, Howard MJ, Nestor-KalinoskiAL, Alberts AS, Eisenmann KM.  (2015) Small Molecule Agonists of Mammalian Diaphanous-related (mDia) Formins Reveal an Effective Glioblastoma Anti-Invasion Strategy.  Mol Biol Cell. Nov;26(21):3704-18.  PMID:  26354425.
  17. McBeth L, Nwaneri AC, Grabnar M, Demeter J, Nestor-Kalinoski A, Hinds TD Jr. (2016) Glucocorticoid Receptor beta Increases Migration of Human Bladder Cancer Cells.  OncotargetMay 10;7(19):27313-24.  doi: 10.18632/oncotarget.8430.  PMID:  27036026.
  18. Wu X, Ren G, Gunning WT 3rd, Weaver DA, Kalinoski AL, Khuder SA, Huntley JF.  (2016)  FmvB: A Francisella tularenisi Magnesium-Responsive Outer Membrane Protein that Plays a Role in Virulence.  PLoS One  Aug11:11(8):e0160977.  doi:10.1371/journal.pone.0160977. PMID:  27513341.
  19. Hinds TD, Jr., Burns KA,  Hosick PA, McBeth,L,  Nestor-Kalinoski, A, Drummond, HA,  AlAmodi, AA Hankins, MW, Vanden Heuvel, JP and Stec, DE. (2016)  Biliverdin reductase A attenuates hepatic steatosis by inhibition of glycogen synthase kinase (GSK) 3β phosphorylation of serine 73 of peroxisome proliferator-activated receptor (PPAR) α.   Journal of Biological Chemistry  291(48):25179-25191.  PMID: 27738106
  20. Marino JS, Stechschulte L, Stec DE, Nestor-Kalinoski A, Coleman S, Hinds Jr TD. (2016) Glucocorticoid Receptor β Induces Hepatic Steatosis by Augmenting Inflammation and Inhibition of PPARα.  Journal of Biological Chemistry 291(50):25776-25788.  PMID:  27784782
  21. Nestor-Kalinoski, A.L.(2016) Second Harmonic Generation and Multiphoton Excited Fluorescence Microscopy as a Phenotypic Tool in an Animal Model. Microscopy and Microanalysis, 22(Suppl 3), 1060–1061. doi: 10.1017/S1431927616006140.  
  22. Hinds, TD Jr., Hosick PA, Hankins MW, Nestor-Kalinoski AL, Stec DE. (2017) Mice with hyperbilirubinemia due to Gilbert’s syndrome polymorphism are resistant to hepatic steatosis by decreased serine 73 phosphorylation of PPARα.  Am J Physiol Endocrinol Metab  Apr 1;312(4):E244-E252.  doi:10.1152/ajpendo.00396.2016. PMID:  28096081
  23. Wyse MM, Goicoechea S, Garcia-Mata R, Nestor-Kalinoski ALand Eisenmann KM.  (2017)  mDia2 and CXCL12/CXCR4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions.  Biochemical and Biophysical Research Communications Mar 4;484(2):255-261. doi: 10.1016/j.bbrc.2017.01.087. PMID: 28115158
  24. Patel J, Ge L, Ahmed S, Ariyarantne M, Phuntumart V, Kalinoski A, Morris PF (2017)  Dual functioning of plant arginases provides a third route for putrescine synthesis.  Plant Science Vol 262: 62-73.
  25. Morran MP, Al-Dieri AG, Nestor‐Kalinoski AL, Jordan RK, Gupta NK and McInerney MF.  (2018) Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes.  Journal of Biological Methods 5(1):e85. DOI:  10.14440/jbm.2018.209
  26. Dvorak KM, Pettee KM, Rubinic-Minotti K, Su R, Nestor-Kalinoski A, Eisenmann KM. (2018) Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).  PLoS One Mar 29;13(3):e0195278. doi:10.1371/journal.pone.0195278.  PMID: 29596520
Last Updated: 2/12/20