University of Toledo Integrated Core Facilities (ICF)

HISTOLOGY Services & PRICING

The Histology Core provides services associated with the microscopic study of the anatomy of cells and tissues from plant or animal origin. The HC offers histological processing, sectioning and staining of frozen or formalin-fixed, paraffin-embedded tissues. In addition, the HC routinely performs staining including: hematoxylin and eosin (H&E), Masson’s Trichrome, PAS (Periodic Acid Schiff), and Oil Red O staining. The HC is fully capable of producing slides for immunofluorescence, immunohistochemistry, or even whole tissue clearing/IDISCO staining for 3D imaging. Custom services, such as, troubleshooting new antibodies to optimize staining protocols are also available. The Histology Core is set up to help accommodate researchers based on their individual needs and can provide services from tissue to digital on frozen or FFPE samples.  The Histology Core houses an Olympus VS120 slide scanner for 1 X 3 slides at 10X, 20X or 40x magnification for brightfield or fluorescence applications.  Slide scans will be uploaded to the server for retrieval and the user will be provided free access to the imaging software for viewing of the full slide scan with optical zoom capabilities for viewing full resolution.

Histology equipment

Leica automatic tissue processor: Provides a more safe and automated system to process tissue and prepare tissue for downstream histological applications. Found in Block Health Science Building, room 007.

Leica EG 1160 embedding station:  Compact automated system to allow consistent reproducible paraffin embedding. Found in Block Health Science Building, room 007.

Reichert-Jung 2030 microtome:  Rotary microtome that allows accurate specimen sectioning, with smooth easily controllable sectioning thickness and speed. Found in Block Health Science Building, room 007.

Thermo Scientific HM355S Automatic Microtome:  Automatic microtome that allows accurate specimen sectioning, with smooth easily controllable sectioning thickness and speed. Found in Block Health Science Building, room 007.

Microm HM550 Cryostat: Cryostat microtome which allows for the production of finely sliced frozen tissue sections. Found in Block Health Science Building, room 007.

Olympus VS120 Slide Scanner Slide Analyzer: Upright microscope which contains a motorized stage for whole tissue scanning in brightfield and fluorescence applications.  Automated microscope which produces high-detail and precise images, with the ability to scan multiple slides. Found in Block Health Science Building, room 007.

Histology equipment For shared use

Leica CM3050 S Cryostat: This cryostat microtome allows for the production of finely sliced frozen tissue sections. Found in Block Health Science Building, room 018.  Please see ICF staff for access.  Cost of use: $30.00/hr.   Instructions for scheduling can be found here.

 Calendar name:

•   Equipment - CoreCryostat Calendar - Advanced Microscopy & Imaging Center

services & rates