Physiology and Pharmacology : Sandrine Pierre

• B.S., Cell Biology/Immunology, 1996, Aix-Marseille II University, France
• M.S., Cell Communication in Endocrinology, 1997, Aix-Marseille II University, France
• Ph.D., Cell Communication in Endocrinology, 2000, Aix-Marseille II University, France
  

• Postdoctoral Fellow, Department of Physiology, Texas Tech University Health Sciences Center, Lubbock, Texas, 2000-2003
• Research Instructor, Department of Physiology, Texas Tech University Health Sciences Center, Lubbock, Texas, February, 2003-August, 2003
• Assistant Professor, Department of Physiology and Pharmacology, University of Toledo College of Medicine, Health Science Campus, September, 2003-

• Molecular basis of the physiological effects of digitalis.

  Na+/K+-ATPase catalyzes the active transport of Na+ and K+ across the plasma membrane.

  To date, studies on the molecular basis of the physiological effects of digitalis such as the increase in cardiac contractility have focused on its ability to specifically inhibit the ion pumping function of Na+/K+-ATPase.

  However, it has become apparent that, in addition to pumping ions, Na+/K+-ATPase interacts with neighbor membrane proteins and takes part in signaling complexes to send messages to various intracellular organelles. Therefore, the mechanism of action of digitalis may go beyond the modulation of ionic homeostasis.

  Accordingly, ongoing studies in the laboratory aim to identify specific intracellular pathways involved in the integrated response of the cell following exposure to digitalis.

Representative Publications

• Pierre, S., Jamme, I., Droy-Lefaix, M-T., Nouvelot, A., and Maixent, J-M. (1999) Ginkgo biloba extract (EGb 761) protects NA,K-ATPase activity during cerebral ischemia in mice. NeuroReport 10, 47-51.
• Sennoune, S., Gerbi, A., Duran, M-J., Benkoel, L., Pierre, S., Lambert, R., Dodero, F., Chamlian, A., Vague, P., and Maixent, J-M. (1999) Quantitative immunocytochemical study of NA,K-ATPase in rat hepatocytes after STZ-induced diabetes and dietary fish oil supplementation. J. Histochem. Cytochem. 47, 809-816.
• Gerbi, A., Sennoune, S., Pierre, S., Sampol, J., Raccah, D., Vague, P., and Maixent, J-M. (1999) Localization of NA+,K+-ATPase alpha/beta isoforms in rat sciatic nerves: Effect of diabetes and fish oil treatment. J. Neurochem. 73, 719-726.
• Barbey, O., Pierre, S., Duran, M-J., Sennoune, S., Levy, S., and Maixent, J-M. (2000) Specific up-regulation of mitochondria F0F1-ATPase activity after short episodes of atrial fibrillation in sheep. J. Cardiovasc. Electrophys. 11, 432-438.
• Maixent, J-M., Barbey, O., Pierre, S., Duran, J-J., Sennoune, S., Bourdeaux, M., Ricard, P., and Levy, S. (2000) Inhibition of NA,K-ATPase by external electrical cardioversion in a sheep model of atrial fibrillation. J. Cardiovasc. Electrophys. 11, 439-445.
• Sennoune, S., Gerbi, A., Duran, M-J., Grillasca, J-P., Compe, E., Pierre, S., Planells, R., Bourdeaux, M., Vague, P., Pieroni, G., and Maixent, J-M. (2000) Effect of streptozotocin-induced diabetes on rat liver NA+/K+-ATPase. Eur. J. Biochem. 267, 2071-2078.
• Pierre, S., Paganelli, F., Sennoune, S., Roche, R., Schwinger, R.H.G., Mesana, T., Maixent, J-M. (2001) RT-PCR detection of the NA,K-ATPase beta-3 isoform in human heart. Cell. Mol. Biol. 47, 261-264.
• Pierre, S., Compe, E., Grillasca, J-P., Plannells, R., Sampol, J., Pressley, T., and Maixent, J-M. (2001) RT-PCR detection of NA,K-ATPase subunit isoforms in human umbilical vein endothelial cells (HUVEC): Evidence for the presence of alpha-1 and beta-3. Cell. Mol. Biol. 47, 319-324.
• Maixent, J.M., Duran, M.J., Pierre, S., Sennoune, S., Robert, K., Bernaqrd, M., and Levy, S. (2002) Remodeling of NA,K-ATPase and membrane fluidity after atrial fibrillation in sheep. J. Recept. Signal Transduct. Res. 22, 203-213.
• Pierre, S.V., Duran, M.J., Carr, D.L., and Pressley, T.A. (2002) Structure/function analysis of NA+-K+-ATPase central isoform-specific region: Involvement in protein kinase C regulation. Am. J. Physiol. Renal Physiol. 283, F1066-F1074.
• Pierre, S., Jamme, I., Robert, K., Gerbi, A., Duran, M-J., Sennoune, S., Droy-Lefaix, MT, Nouvelot, A., and Maixent, J-M. (2002) Ginkgo biloba extract (EGb 761) protects NA,K-ATPase isoenzymes during cerebral ischemia. Cell. Mol. Biol. 48, 671-679.
• Pierre, S.V., Duran, M.J., Carr, D.L., and Pressley, T.A. (2003) Structure/function analysis of Na,K-ATPase alpha1 and alpha2 central isoform-specific regions reveals their involvement in regulation by protein kinase C. Ann. N.Y. Acad. Sci. 986, 260-262.
• Duran, M.J., Pierre, S.V., Carr, D.L., and Pressley, T.A. (2003) Role of the isoform-specific region of the Na,K-ATPase catalytic subunit. Ann. N.Y. Acad. Sci. 986, 258-259.
• Duran, M.J., Pierre, S.V., Carr, D.L., and Pressley, T.A. (2004) The isoform-specific region of the Na,K-ATPase catalytic subunit: Role in enzyme kinetics and regulation by protein kinase C. Biochemistry 43, 16174-16183.
• Pressley, T.A., Duran, M.J., and Pierre, S.V. (2005) Regions conferring isoform-specific function in the catalytic subunit of the Na,K-pump. Front. Biosci. 10, 2018-2026.
• Liu, L., Li, J., Liu, J., Yuan, Zhaokai, Pierre, S.V., Qu, W., Zhao, X., and Xie, Z.  (2006)  Involvement of Na+/K+-ATPase in hydrogen peroxide-induced hypertrophy in cardiac myocytes.  Free Radical Biology & Medicine 41, 1548-1556. 
• Pasdois, P., Quinlan, C.L., Rissa, A., Tariosse, L., Vinassa, B., Pierre, S.V., Dos Santos, P., and Garlid, K.D. (2007) Ouabain protects rat hearts against ischemia-reperfusion injury via a pathway involving Src kinase, mitoKATP, and ROS. Am. J. Physiol. (Heart Circ. Physiol.) 292:H1470-H1478.
• Pierre, S.V. and Xie, Z.-J. (2006) The Na,K-ATPase Receptor Complex: Its Organization and Membership. Cell Biochem Biophys. 46(3):303-316.
• Pierre, S.V., Yang, C., Yuan, Z., Seminerio, J., Mouas, K., Dos Santos, P., and Xie, Z.J. (2007) Ouabain triggers preconditioning through activation of the Na+,K+-ATPase signaling cascade in rat hearts.  Cardiovasc Res. Feb 1;73(3), 488-96. Epub 2006 Nov 6.
• Liang, M., Tian, J., Liu, L., Pierre, S., Liu, J., Shapiro, J., and Xie, Z.J. (2007) Identification of a pool of non-pumping Na/K-ATPase.  J Biol Chem. 282:10585-10593.
• Bonello, L., Sbragia, P., Amabile, N., Com, O., Pierre, S.V., Levy, S., and Paganelli, F.  (2007)  Protective effect of an acute oral loading dose of trimetazidine on myocardial injury following percutaneous coronary intervention. Heart 93: 703-707.
• Liu, L., Zhao, X., Pierre, S.V., and Askari, A.  (2007)  Association of PI3K-Akt signaling pathway with digitalis-induced hypertrophy of cardiac myocytes.  Am J Physiol Cell Physiol. 293(5):C1489-497.
• Costa, A.D., Pierre, S.V., Cohen, M.V., Downey, J.M. and Garlid, K.D.  (2008)  cGMP signaling in pre- and post-conditioning: the role of mitochondria.  Cardiovasc. Res. Jan 15; 77(2);344-352.
• Pierre, S.V., Sottejeau, Y., Gourbeau, J.M., Sánchez, G., Shidyak, A. and Blanco, G.  (2008) Isoform specificity of Na-K-ATPase-mediated ouabain signaling.  Am J Physiol Renal Physiol. Apr; 294(4):F859-F866. Epub 2007 Dec 19.
• Quinlan, C.L., Costa, A.D., Costa, C.L., Pierre, S.V., Dos Santos, P. and Garlid, K.D.  Conditioning the Heart Induces Formation of Signalosomes that Interact with Mitochondria to Open MitoKATP.  American Journal of Physiology (Heart Circulation Physiology) 295:953-961, 2008.
• Pierre, S.V., Lesnik, P., Moreau, M., Bonello, L., Droy-Lefaix, M.T., Sennoune, S., Duran, M.J., Pressley, T.A., Sampol, J. Chapman, J. and Maixent J.M.   (2008)  The standardized Ginkgo biloba extract Egb-761 protects vascular endothelium exposed to oxidized low density lipoproteins.  Cell Mol Biol (Noisy-le-grand). Oct 26; 54:1032-1042 Suppl:OL1032-42.
• Garlid, K.D., A.D. Costa, C.L. Quinlan, S.V. Pierre, P. Dos Santos.  (2009) Cardioprotective signaling to mitochondria. J Mol Cell Cardiol. Jun; 46(6):858-66.