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Health Education Building & Center for Creative Education Building
BPG Computer Classroom: HEB 1st Floor, Room #127
Genomic Core Lab: HEB 2nd Floor, Room #200
BPG Office: CCE 3rd Floor, Lobby
We suggest that you contact us directly, if you would like to design a custom microarray
project. Please contact the GCL Director by email, email@example.com, or by phone: 419-383-6105.
What you need to provide for the Affymetrix System
We recommend that you provide biotin-labeled fragmented aRNA in a quantity sufficient to allow for one time application of the hybridization cocktail to each microarray cartridge. For example, a single human sample to be run on the GeneChip® Human Genome U133 Plus 2.0 Array would require 12.5 µg of biotin-labeled aRNA after completion of the Affymetrix protocol. Specific requirements for paperwork accompanying the sample are listed below.
Minimally, we can accept 12.5 µg of Affymetrix kit-prepared aRNA for creating a hybridization cocktail that, if needed, can be reused on several chips. Affymetrix has informed us that a hybridization cocktail may be used up to 5 times without significant loss of signal for transcripts of average abundance.
Required information with submitted sample:
Information may be obtained by the following method, or can be substituted with appropriate information from instrumentation, such as the Agilent Technologies 2100 Bioanalyzer.