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~5.5yr with Dept. of Biological
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- Genomics Core Lab
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Graduate School Handbook
Health Science Campus
Health Education Building & Center for Creative Education Building
BPG Computer Classroom: HEB 1st Floor, Room #127
Genomic Core Lab: HEB 2nd Floor, Room #200
BPG Office: CCE 3rd Floor, Lobby
These services are provided by the Genomics Core Laboratory:
Affymetrix Microarray Analysis
The GCL performs gene expression analyses with the Affymetrix GeneChip system. Standard Affymetrix protocols are used by the investigator to prepare biotin-labeled cRNA starting from either total RNA or mRNA. At present, the GCL can accept only prepared biotin-labeled, fragmented cRNA samples, and carries out hybridization of the prepared samples, post-hybridization washing and staining, and scanning of fluorescence levels utilizing a GeneArray™ 3000 (6G) scanner.
The most commonly used Affymetrix GeneChip® arrays are (following 3 links are in pdf format): Human (GeneChip® Human Genome U133 Plus 2.0 Array analyzes expression levels of over 47,000
transcripts and variants, including 38,500 well-characterized human genes), Mouse (GeneChip® Mouse Genome 430 2.0 Array analyzes 39,000 transcripts and variants from
over 34,000 well-characterized mouse genes), and Rat (GeneChip® Rat Genome 230 2.0 Array analyzes over 30,000 transcripts and variants
from over 28,000 well-substantiated rat genes). The Human, Mouse, and Rat GeneChips
cost approximately $400 per chip and are ordered by the customer (please contact us
prior to placing an order). Other Affymetrix genome arrays are available for a number
of organisms. The GCL processing cost of $100 for each sample covers chip hybridization
and scanning, and preliminary data analysis.
We strongly encourage users to consult with us on the planning of their GeneChip experiments. We will gladly offer our advice in the experimental design, choice of chips, sample preparation, and coordination of experiment timings. You can contact the GCL Director by e-mail email@example.com, or by phone:
419-383-6105. He is also available to speak to your research team or department about the GCL and microarray analysis.
PerkinElmer Microarray Analysis
Processed, hybridized, spotted arrays in a microscope slide format can be brought to the GCL to be scanned. Slides are scanned by a PerkinElmer ScanArray Express Microarray Analysis System that uses 543 nm and 633 nm lasers for excitation intensities of the labeled hybridized samples. PerkinElmer™ ProScan Array Express Microarray Analysis software is used for image analysis, with the GCL staff assisting researchers in its use.
If you would like to design a custom microarray project, please contact the GCL Director by e-mail firstname.lastname@example.org, or by phone: 419-383-6105.