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Salah-uddin Ahmed 419-530-1913 office |
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B.S. Rajasthan University, Jaipur, India
Zoology and Chemistry
M.S. Jamia Hamdard University, New Delhi, India
Toxicology
Ph.D. Jamia Hamdard University, New Delhi, India
Post-Doc Case Western Reserve University
Orthopedics
My primary research interest is to test the efficacy of green tea and its active compound epigallocatechin gallate (EGCG) in rheumatoid arthritis (RA). Using synovial fibroblasts isolated from human RA joints, we are studying the mechanism of green tea and EGCG mediated regulation of chemokine production and chemokine receptor expression, and inhibition of synovial fibroblast invasion. Furthermore, we are also testing the therapeutic potential of EGCG in regulating cell recruitment, angiogenesis and joint destruction in animal models of RA and in RA synovial tissue (ST)-severe combined immunodeficient (SCID) chimera by blocking chemokine receptor CCR1/CCR5 expression. Patients suffering from RA tend to develop cardiovascular complications. My research focus in this area is to study the role of pro-inflammatory cytokines and downstream inflammatory mediators in the manifestation of cardiovascular complications in RA. Based on these findings, we plan to test potential novel anti-inflammatory molecules in clinical intervention studies. The success of these studies may lead to a significant advancement in the development of EGCG or structurally related molecules as potential treatment options for RA and possibly other autoimmune diseases. View Dr. Ahmed's complete CV
Ahmed S, Rahman A, Hasnain A, Lalaonde M, Goldberg VM, Haqqi TM: Green tea polyphenol epigallocatechin-3-gallate inhibits the IL-1β-induced activity and expression of cyclooxygenase-2 and nitric oxide synthase-2 in human chondrocytes. Free Radic. Biol. Med. 33:1097-1105, 2002.
Singh R*, Ahmed S*, Islam N, Goldberg VM, Haqqi TM: Epigallocatechin-3-gallate inhibits interleukin-1β-induced expression of nitric oxide synthase and production of nitric oxide in human chondrocytes: suppression of nuclear factor-κB (NF-κB/p65) activation by inhibiting ΙκΒ−α degradation. Arthritis Rheum. 46:2079-2086, 2002. (*Equal contribution).
Ahmed S, Rahman A, Husnain A, Goldberg VM, Haqqi TM: Phenyl-N-tert-butylnitrone (PBN) down- regulates IL-1β-stimulated activation of matrix metalloproteinase-13 (MMP-13) gene expression in human chondrocytes: Suppression of c-Jun NH2-terminal kinase, p38-MAPK and activating protein-1 (AP-1). J. Pharmacol Exp. Ther. 305:981-988, 2003.
Ahmed S, Wang N, Lalanode MS, Khan SA, Goldberg VM, Haqqi TM: Differential inhibition of interleukin-1β-induced expression of matrix metalloproteinases-1 and -13 by green tea polyphenol epigallocatechin-3-gallate (EGCG) in human chondrocytes. J. Pharmacol Exp. Ther. 308:767-773, 2004.
Wang N, Ahmed S, Haqqi TM: Genomic structure and functional characterization of the promoter region of human IκB kinase-related kinase IKK-i/IKKε gene. Gene 353:118-133, 2005.
Ahmed S, Wang N, Haqqi TM: Punica granatum L extract inhibits IL-1β-induced expression of matrix metalloproteinases in human chondrocytes by inhibiting the activation of mitogen-activated protein kinases sub-groups p38-MAPK and JNK and the nuclear factor-κB Activity. J. Nutr. 135:2096-2102, 2005.
Ahmed S, Anuntiyo J, Malemud CJ, Haqqi TM: Biological basis for the use of botanicals in osteoarthritis and rheumatoid arthritis: a review. Evid. Based Complement. Alternat. Med. 2:301-308, 2005.
Hafeez BB, Ahmed S, Wang N, Gupta S, Haqqi TM: Green tea polyphenols induce apoptosis in SAOS-2 cells via inhibition of nuclear factor-κB (NF-κB) and activation of caspase-3. Toxicol. Appl. Pharmacol. 216:11-19, 2006.
Ahmed S*, Pakozdi A, Koch AE: Regulation of interleukin-1β-induced chemokine production and matrix metalloproteinase-2 (MMP-2) activation by epigallocatechin-3-gallate (EGCG) in rheumatoid arthritis synovial fibroblasts. Arthritis Rheum. 54:2393-2401, 2006. [*Corresponding author].
Amin MA, Mansfield PJ, Pakozdi A, Campbell PL, Ahmed S, Martinez RJ, Koch AE: Interleukin-18 induces angiogenic factors in rheumatoid arthritis synovial tissue fibroblasts via distinct signaling pathways. Arthritis Rheum. 56:1817-1826, 2007.
Haas CS, Amin MA, Ruth JH, Allen BA, Ahmed S, Pakozdi A, Woods JM, Shahrara S, Koch AE: In vivo inhibition of angiogenesis by interleukin-13 gene therapy in a rat model of rheumatoid arthritis Arthritis Rheum. 56:2535-2548, 2007.
Ahmed S*, Marotte H, Kwan K, Ruth JH, Campbell PJ, Rabquer BJ, Pakozdi A, Koch AE: Epigallocatechin-3-gallate (EGCG) inhibits IL-6 synthesis and suppresses trans-signaling by enhancing soluble gp130 production. Proc Natl Acad Sci USA. 105:14692-14697, 2008. [*Corresponding author].
Ahmed S*, Silverman MD, Hubert M, Kwan K, Matuszczak N, Koch AE: Down-regulation of Mcl-1 by epigallocatechin-3-gallate (EGCG) sensitizes rheumatoid arthritis synovial fibroblasts to tumor necrosis factor-alpha (TNF-a)-induced apoptosis. Arthritis Rheum. 60:1282-1293, 2009. [* Corresponding author].
Marotte H, Ruth JH, Campbell PJ, Koch AE, Ahmed S: Green tea extract inhibits chemokine production, but upregulates chemokine receptor expression, in rheumatoid arthritis synovial fibroblasts and rat adjuvant-induced arthritis. Rheumatology 2009 [Accepted].
Marotte H, Ahmed S, Ruth JH, Koch AE: Blocking ERK1/2 reduces TNF-α-induced IL-18 bioactivity in rheumatoid arthritis synovial fibroblasts by induction of IL-18BPa. Arthritis Rheum. 2009 [In press].