Biochemistry & Cancer Biology

Sandrine Pierre, Ph.D.



Sandrine V. Pierre, Ph.D.
Assistant Professor
Biochemistry & Cancer Biology

Research Interests

The integrity of Na+,K+-ATPase protein complexes at the cell surface of the cardiac myocyte is not only critical for the maintenance of ion homeostasis, it is also key to adequate initiation and transduction of digitalis signalling and subsequent pharmacological effects. We focus on the mechanism of ischemia/reperfusion-induced Na+,K+-ATPase alteration and preconditioning by digitalis as tools to develop novel approaches for therapeutic intervention in myocardial infarction.

Member of the mentoring faculty for the Biomedical Sciences Graduate Program (Molecular Medicine).


B.S., Cell Biology/Immunology, 1996, Aix-Marseille II University, France
M.S., Cell Communication in Endocrinology, 1997, Aix-Marseille II University, France
Ph.D., Cell Communication in Endocrinology, 2000, Aix-Marseille II University, France


2000-2003 Postdoctoral Fellow, Dept of Physiology, Texas Tech University Health Sciences Center, Lubbock, TX
2/2003-8/2003 Research Instructor, Dept of Physiology, Texas Tech University Health Sciences Center, Lubbock, TX
2003-2011 Assistant Professor, Dept of Physiology and Pharmacology, Univ. of Toledo College of Medicine, Health Science Campus, Toledo, OH
2001-present Assistant Professor, Dept of Biochemistry & Cancer Biology, Univ. of Toledo College of Medicine & Life Sciences, Toledo, OH
Representative Publications

Pierre SV, Belliard A, Sottejeau Y. (2011) Modulation of Na(+)-K(+)-ATPase cell surface abundance through structural determinants on the α1-subunit.  Am J Physiol Cell Physiol. Jan;300(1):C42-8. Epub 2010 Nov 3.

Duran MJ, Pierre SV, Lesnik P, Pieroni G, Bourdeaux M, Dignat-Georges F, Sampol J, Maixent JM. (2010) 7-ketocholesterol inhibits Na,K-ATPase activity by decreasing expression of its α1-subunit and membrane fluidity in human endothelial cells. Cell Mol Biol (Noisy-le-grand). Nov 9;56 Suppl:OL1434-41.

Sottejeau Y, Patel AM, Gerber G, Pierre SV, Maixent JM.  (2010) Effect of a novel Omegacoeur®/Doluperine® nutritional combination on human embryonic kidney cell viability. Cell Mol Biol (Noisy-le-grand). Oct 5;56 Suppl:OL1400-9.

Quintas LE, Pierre SV, Liu L, Bai Y, Liu X, Xie ZJ. (2010) Alterations of Na+/K+-ATPase function in caveolin-1 knockout cardiac fibroblasts. J Mol Cell Cardiol. 2010 Sep;49(3):525-31. Epub 2010 May 6.

Sottejeau Y, Belliard A, Duran MJ, Pressley TA, Pierre SV. (2010) Critical role of the isoform-specific region in alpha1-Na,K-ATPase trafficking and protein Kinase C-dependent regulation. Biochemistry May 4;49(17):3602-10.

Morgan EE, Li Z, Stebal C, Belliard A, Tennyson G, Salari B, Garlid KD, Pierre SV. (2010) Preconditioning by subinotropic doses of ouabain in the Langendorff perfused rabbit heart. J Cardiovasc Pharmacol. 2010 Mar;55(3):234-9.

Garlid, K.D., A.D. Costa, C.L. Quinlan, S.V. Pierre, P. Dos Santos. (2009) Cardioprotective signaling to mitochondria. J Mol Cell Cardiol. Jun; 46(6):858-66.

Pierre, S.V., Lesnik, P., Moreau, M., Bonello, L., Droy-Lefaix, M.T., Sennoune, S., Duran, M.J., Pressley, T.A., Sampol, J. Chapman, J. and Maixent J.M. (2008) The standardized Ginkgo biloba extract Egb-761 protects vascular endothelium exposed to oxidized low density lipoproteins. Cell Mol Biol (Noisy-le-grand). Oct 26; 54:1032-1042 Suppl:OL1032-42.

Quinlan, C.L., Costa, A.D., Costa, C.L., Pierre, S.V., Dos Santos, P. and Garlid, K.D. (2008) Conditioning the Heart Induces Formation of Signalosomes that Interact with Mitochondria to Open MitoKATP. American Journal of Physiology (Heart Circulation Physiology) 295:953-961.

Pierre, S.V., Sottejeau, Y., Gourbeau, J.M., Sánchez, G., Shidyak, A. and Blanco, G. (2008) Isoform specificity of Na-K-ATPase-mediated ouabain signaling. Am J Physiol Renal Physiol. Apr; 294(4):F859-F866. Epub 2007 Dec 19.

Costa, A.D., Pierre, S.V., Cohen, M.V., Downey, J.M. and Garlid, K.D. (2008) cGMP signaling in pre- and post-conditioning: the role of mitochondria. Cardiovasc. Res. Jan 15; 77(2);344-352.

Liu, L., Zhao, X., Pierre, S.V., and Askari, A. (2007) Association of PI3K-Akt signaling pathway with digitalis-induced hypertrophy of cardiac myocytes. Am J Physiol Cell Physiol. 293(5):C1489-497.

Bonello, L., Sbragia, P., Amabile, N., Com, O., Pierre, S.V., Levy, S., and Paganelli, F. (2007) Protective effect of an acute oral loading dose of trimetazidine on myocardial injury following percutaneous coronary intervention. Heart 93: 703-707.

Liang, M., Tian, J., Liu, L., Pierre, S., Liu, J., Shapiro, J., and Xie, Z.J. (2007) Identification of a pool of non-pumping Na/K-ATPase. J Biol Chem. 282:10585-10593.

Pierre, S.V., Yang, C., Yuan, Z., Seminerio, J., Mouas, K., Dos Santos, P., and Xie, Z.J. (2007) Ouabain triggers preconditioning through activation of the Na+,K+-ATPase signaling cascade in rat hearts. Cardiovasc Res. Feb 1;73(3), 488-96. Epub 2006 Nov 6.

Pierre, S.V. and Xie, Z.-J. (2006) The Na,K-ATPase Receptor Complex: Its Organization and Membership. Cell Biochem Biophys. 46(3):303-316.

Pasdois, P., Quinlan, C.L., Rissa, A., Tariosse, L., Vinassa, B., Pierre, S.V., Dos Santos, P., and Garlid, K.D. (2007) Ouabain protects rat hearts against ischemia-reperfusion injury via a pathway involving Src kinase, mitoKATP, and ROS. Am. J. Physiol. (Heart Circ. Physiol.) 292:H1470-H1478.

Liu, L., Li, J., Liu, J., Yuan, Zhaokai, Pierre, S.V., Qu, W., Zhao, X., and Xie, Z. (2006) Involvement of Na+/K+-ATPase in hydrogen peroxide-induced hypertrophy in cardiac myocytes. Free Radical Biology & Medicine 41, 1548-1556.

Pressley, T.A., Duran, M.J., and Pierre, S.V. (2005) Regions conferring isoform-specific function in the catalytic subunit of the Na,K-pump. Front. Biosci. 10, 2018-2026.

Duran, M.J., Pierre, S.V., Carr, D.L., and Pressley, T.A. (2004) The isoform-specific region of the Na,K-ATPase catalytic subunit: Role in enzyme kinetics and regulation by protein kinase C. Biochemistry 43, 16174-16183.

Duran, M.J., Pierre, S.V., Carr, D.L., and Pressley, T.A. (2003) Role of the isoform-specific region of the Na,K-ATPase catalytic subunit. Ann. N.Y. Acad. Sci. 986, 258-259.

Pierre, S.V., Duran, M.J., Carr, D.L., and Pressley, T.A. (2003) Structure/function analysis of Na,K-ATPase alpha1 and alpha2 central isoform-specific regions reveals their involvement in regulation by protein kinase C. Ann. N.Y. Acad. Sci. 986, 260-262.

Pierre, S., Jamme, I., Robert, K., Gerbi, A., Duran, M-J., Sennoune, S., Droy-Lefaix, MT, Nouvelot, A., and Maixent, J-M. (2002) Ginkgo biloba extract (EGb 761) protects NA,K-ATPase isoenzymes during cerebral ischemia. Cell. Mol. Biol. 48, 671-679.

Pierre, S.V., Duran, M.J., Carr, D.L., and Pressley, T.A. (2002) Structure/function analysis of NA+-K+-ATPase central isoform-specific region: Involvement in protein kinase C regulation. Am. J. Physiol. Renal Physiol. 283, F1066-F1074.

Maixent, J.M., Duran, M.J., Pierre, S., Sennoune, S., Robert, K., Bernaqrd, M., and Levy, S. (2002) Remodeling of NA,K-ATPase and membrane fluidity after atrial fibrillation in sheep. J. Recept. Signal Transduct. Res. 22, 201-211.

Pierre, S., Compe, E., Grillasca, J-P., Plannells, R., Sampol, J., Pressley, T., and Maixent, J-M. (2001) RT-PCR detection of NA,K-ATPase subunit isoforms in human umbilical vein endothelial cells (HUVEC): Evidence for the presence of alpha-1 and beta-3. Cell. Mol. Biol. 47, 319-324.

Pierre, S., Paganelli, F., Sennoune, S., Roche, R., Schwinger, R.H.G., Mesana, T., Maixent, J-M. (2001) RT-PCR detection of the NA,K-ATPase beta-3 isoform in human heart. Cell. Mol. Biol. 47, 261-264.

Sennoune, S., Gerbi, A., Duran, M-J., Grillasca, J-P., Compe, E., Pierre, S., Planells, R., Bourdeaux, M., Vague, P., Pieroni, G., and Maixent, J-M. (2000) Effect of streptozotocin-induced diabetes on rat liver NA+/K+-ATPase. Eur. J. Biochem. 267, 2071-2078.

Maixent, J-M., Barbey, O., Pierre, S., Duran, J-J., Sennoune, S., Bourdeaux, M., Ricard, P., and Levy, S. (2000) Inhibition of NA,K-ATPase by external electrical cardioversion in a sheep model of atrial fibrillation. J. Cardiovasc. Electrophys. 11, 439-445.

Barbey, O., Pierre, S., Duran, M-J., Sennoune, S., Levy, S., and Maixent, J-M. (2000) Specific up-regulation of mitochondria F0F1-ATPase activity after short episodes of atrial fibrillation in sheep. J. Cardiovasc. Electrophys. 11, 432-438.

Gerbi, A., Sennoune, S., Pierre, S., Sampol, J., Raccah, D., Vague, P., and Maixent, J-M. (1999) Localization of NA+,K+-ATPase alpha/beta isoforms in rat sciatic nerves: Effect of diabetes and fish oil treatment. J. Neurochem. 73, 719-726.

Sennoune, S., Gerbi, A., Duran, M-J., Benkoel, L., Pierre, S., Lambert, R., Dodero, F., Chamlian, A., Vague, P., and Maixent, J-M. (1999) Quantitative immunocytochemical study of NA,K-ATPase in rat hepatocytes after STZ-induced diabetes and dietary fish oil supplementation. J. Histochem. Cytochem. 47, 809-816.

Pierre, S., Jamme, I., Droy-Lefaix, M-T., Nouvelot, A., and Maixent, J-M. (1999) Ginkgo biloba extract (EGb 761) protects NA,K-ATPase activity during cerebral ischemia in mice. NeuroReport 10, 47-51.

Last Updated: 6/9/16