Sudhir Jain, Ph.D.
- 1989, B.Sc. (Zoology, Chemistry, Environmental Science), Delhi University, Delhi, India
- 1991, M.Sc., (Zoology), Sagar University, Sagar, India
- 1997, Ph.D., Life Sciences (Reproductive Biology) Devi Ahilya University, Indore, India
- Research Scholar (Ph.D.), 1992-1997, School of Life Sciences, DAVV, Indore, India
- Postdoctoral Research Associate, 1997-1999, National Institute of Immunology, Delhi, India.
- Postdoctoral Fellow, January 2000-October 2000, Animal Science Department, Rutgers University, New Brunswick, New Jersey.
- Postdoctoral Associate, October 2000-December 2003, New York Medical College, Valhalla, New York.
- Research Assistant Professor, January 2004-September 2011, New York Medical College, Valhalla, New York.
- Assistant Professor, October 2011-present, University of Toledo, Toledo, Ohio.
Transcriptional regulation of genes associated with Human Hypertension
My laboratory strives to evaluate the contributions of genetic heterogeneity in pathological conditions associated with the cardiovascular-renal systems. In particular, we study single nucleotide polymorphisms in the renin-angiotensin-aldosterone (RAA) axis with an eye towards their impact in the genesis of hypertension; our long term goal being to identify novel molecular targets in this pathway so as to facilitate the development of a "tailored" drug therapy for hypertension and associated pathologies. We have cloned and generated mice models of human angiotensinogen gene and its receptor hAT1R gene variants. We employ these animal models to dissect differential regulation of these target genes whose polymorphisms have been reported in the human population. Apart from blood pressure regulation, we have proposed the use of these animal models, with appropriate modifications, to better understand the role of RAA axis in contributing towards metabolic syndrome and vascular lesions of the brain and the kidneys. In this regard, we have preliminary evidence linking our target genes to the development of metabolic syndrome and associated cardio-renal pathologies. Major focus of our work is to analyze how metabolic syndrome affects cellular gene regulatory networks and hAGT/hAT1R expression in our transgenic lines. These studies will improve our understanding of the metabolic syndrome-RAS interplay, identify “at risk” patients, and provide supplementary therapeutic options for patients with metabolic syndrome.
Selected Journal Publications
Sudhir Jain, Jatin Tulsulkar, Anita Rana, Ashok Kumar and Zahoor Shah (2015) Transgenic mice overexpressing angiotensin 1 receptor are susceptible to stroke injury. Molecular Neurobiology (accepted DOI 10.1007/s12035-015-9109-2). (Corresponding Author). PMID: 25652270
Varunkumar G. Pandey, Sudhir Jain , Anita Rana , Nitin Puri , Sri Krishna C. Arudra , Brahmaraju Mopidevi, Meenakshi Kaw, Alberto Nasjletti, and Ashok Kumar (2015) Dexamethasone Promotes Hypertension By Allele-Specific Regulation Of The Human Angiotensinogen Gene. The Journal of Biological Chemistry 290(9):5749-5758. PMID: 25568318
Mopidevi BR, Kaw M, Puri N, Ponnala M, Jain S, Rana A, Keetha NR, Fiering SN, and Kumar A (2015) Variable transcriptional regulation of the human aldosterone synthase gene causes salt-dependent high blood pressure in transgenic mice. Circulation: Cardiovascular Genetics 2015 Feb; 8(1):30-39. PMID: 25504670
Sudhir Jain, Alicia Prater, Varunkumar G Pandey, Anita Rana, Nitin Puri and Ashok Kumar (2013) A haplotype of angiotensin receptor associated with human hypertension increases blood pressure in transgenic mice. The Journal of Biological Chemistry 288(52):37048-37056. PMID: 24202179
Sudhir Jain, Andrej Tillinger, Brahmaraju Mopidevi, Varunkumar G Pandey, Chetan KC Chauhan, Steven Fiering, Soren Warming and Ashok Kumar (2010) Transgenic mice with -6A haplotype of the human angiotensinogen gene have increased blood pressure compared to -6G haplotype. The Journal of Biological Chemistry 285:41172-41186. PMID: 20978123
Sudhir Jain, Govindaiah Vinukonda, Steven Fiering, and Ashok Kumar (2008) A haplotype of human angiotensinogen gene containing -217A increases blood pressure in transgenic mice as compared to – 217G. American Journal of Physiology-Regulatory Integrative Comp Physiol. 295:1849-1857. PMID: 18945948
Sudhir Jain, Yana li, Sai Patil, and Ashok Kumar (2007) HNF-1α plays an important role in IL-6 induced expression of the human angiotensinogen gene. American Journal of Physiology-Cell Physiology 293:C401-C410 (Corresponding Author). PMID: 17475670
Sudhir Jain, Mehul Shah, Yanna Li, Govind Vinukonda, Pravin B Sehgal, and Ashok Kumar (2006) Interferon-γ increases the expression of Human Angiotensinogen Gene. BBA-Gene Structure and Expression, 1759:340-347. PMID: 16949687
Yana Li, Sudhir Jain, Sai Patil, and Ashok Kumar (2006) A haplotype of angiotensinogen gene is associated with essential hypertension and effects promoter activity in adipocytes. Vascular Pharmacology, 44:29-33. PMID: 16303336
Jain S, Li Y, Kumar A, and Sehgal PB (2005) Transcriptional signaling from membrane raft-associated glucocorticoid receptor (GR). BBRC, 336(1):3-8. PMID: 16125141
Ashok Kumar, Yanna Li, Sai Patil, and Sudhir Jain (2005) A haplotype of the angiotensinogen geneis associated with hypertension in African-Americans. Clinical and Experimental Pharmacology and Physiology, 32:95-502. PMID: 15854165
Sudhir Jain, Yana Li, Sai Patil, and Ashok Kumar (2005) A SNP in hAGT gene is associated with essential hypertension and effects glucocorticoid induced promoter activity. Journal of Molecular Medicine (JMM), 83(2):121-135.
Sudhir Jain, Xiangna Tang, Chittampalli S. Narayanan, Yogesh Aggarwal, Stephen M Peterson, Clinton D Brown, Jurg Ott, and Ashok Kumar (2002) Angiotensinogen gene polymorphism at -217 affects basal promoter activity and is associated with hypertension in african American. The Journal of Biological Chemistry, 277(39):36889-36896. PMID: 12145290