Cancer Biology Track

David C. Allison, M.D., Ph.D.



M.D.   1967 University of Michigan
Ph.D.  1976 University of Chicago (Microbiology)

Positions and Honors:

1967-1968       Surgical Intern, University of Chicago
1968-1970       Medical Officer, U.S. Army
1970-1976       Graduate Student, University of Chicago
1974-1977       Surgical Resident, University of Chicago
1977-1978       Instructor of Surgery, University of Chicago
1978-1986       Assistant to Associate Professor of Surgery,
                        University of New Mexico School of Medicine
1978-1986       Staff Physician, Albuquerque Veterans Administration Medical Center
1986-1982       Associate Professor of Surgery and Oncology,
                        The Johns Hopkins University School of Medicine
1986-1992       Chief of Surgical Oncology, The Johns Hopkins Hospital
1986-1992       Staff Physician, Baltimore VAMC
1992-1996       Professor and Chairman, Department of Surgery, Medical College of Ohio
1992-Present   Lecturer in Surgery, The Johns Hopkins University School of Medicine
1997-2005       Professor of Surgery, Physiology and Molecular Medicine,
                        Medical College of Ohio
1997-Present   Director, Surgical Research
2004-2005       Acting Chairman, Department of Surgery, Medical University of Ohio
2006-Present   Director, University of Toledo Advanced Microscopy and Imaging Center

Editorial Boards:

1984-1988       J Histochemistry and Cytochemistry
1987-1992       Cell and Tissue Kinetics
1987-1992       Cell Proliferation
1992-present   J Surgical Oncology


American Association for the Advancement of Science
American Association for Cancer Research
American Surgical Association
Histochemical Society
Sigma Xi Society
Society of University of Surgeons
Society of Surgical Oncologists
Surgical Biology Club II
N.I.H. Site Visit Reviewer, "Extension of Radiotherapy Research" M.D. Anderson
     Hospital, Houston, TX  January 1991
VA Merit Review Program, Program Specialist in Surgery, Veterans Health Services and
     Research Administration, July 1991-1995
American College of Surgeons Commission on Cancer, 1990-2000
Examination Consultant, American Board of Surgery, Surgical Basic Science Section, 1989-1999 


1972-1974          NIH Fellow, University of Chicago
1994-1995          President, Cell Kinetics Society

Selected Peer Reviewed Publications:

Allison, DC, Abell M, Payne FE, Accelerated skin graft rejection following transfer of cells sensitized to a “weak” histocompatibility antigen(s).  Immunological Comm. 2(4):393-402, 1973.

Allison, DC, M Ralph, et al. Partial separation of Sendai virus-fused lymphocytes by velocity sedimentation. J Immunol Methods 5:121-9. 1974.

Allison DC, McKearn T.  A mechanism for the generation of self‑tolerance.  J Theor Biol 54:201-6, 1975.

Allison DC, Meier P, Majeune M, Cohen E.  Antigen expression of thymocyte X spleen cell heterokaryons.  Cell 6:521-7, 1975.

Allison DC, Bose K, Hill JL.  Detection of thymic surface antigens and radioactive labeling of mouse lymphoid cells: J Histochem Cytochem 25:1269-74, 1977. 

Allison DC, Ridolpho P.  Use of a trypan blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable cells.  J Histochem Cytochem 28:700-3, 1980.

Allison DC, Ridolpho P, Rasch E, Rasch R, Johnson T. Increased accuracy of absorption cytophotometric DNA values by control of stain intensity.  J Histochem Cytochem 29:1219-28, 1981.

Allison DC, Yuhas JM, Ridolpho PF, Anderson SL, Johnson TS. Cytophotometric measurement of the cellular DNA content of (3H)thymidine‑labeled spheroids:  Direct demonstration of non‑labeled cells with S and G2 DNA content.  Cell Tissue Kinet 16:237-46, 1983.

Allison DC, Meyne J, Ridolpho PF, Bose K, Chakerian M, Robertson J.  Computerized measurement of the DNA content, areas, and autoradiographic grains of the same nuclei. J Histochem Cytochem 32:1197, 1984.

Allison DC, Lawrence GN, O'Grady BJ, Rasch RW, Rasch EM.  Increased accuracy and speed of absorption cytometric DNA Measurements by automatic correction for nuclear darkness. Cytometry 5:217-27, 1984.

Bose K, Allison DC.  An improved method of preparing nuclei for absorption cytometry.  J Histochem Cytochem 33:65-8, 1985.

Meyne J, Allison DC, Bose K, Jordan SW, Ridolpho PF, Smith J. Hepatotoxic doses of dioxin  do not damage mouse bone marrow chromosomes.  Mutation Research 157:63-9, 1985.

Allison DC.  Refinements in absorption‑cytometric measurements of cellular DNA content.  In: Advances in Microscopy.  Edited by Cowden RR, Harrison FW, AR Liss Company, NY, 1985, pp 167‑85.

Allison DC, Ridolpho PF, Anderson S, Bose K.  Variations in the (3H)thymidine‑labeling of S‑phase cells in experimental mouse tumors.  Cancer Research 45:6010-16, 1985. 

Allison DC, Anderson S, Meyne J, Ridolpho PF, Robertson J. Alterations in the DNA metabolism of MCa‑11 tumor cells grown in vivo and in vitro.  Cancer Research 46:3951-7, 1986.

Curley, SA and Allison, DC. Alterations in cell cycle kinetics with changes in tumor size.  Surgical Forum 37:407-8, 1986.

Allison DC, Chakerian M, Ridolpho P, Curley S, Wilder M, Anderson S, Robertson J.   Combined flow and absorption DNA measurements of (3H)thymidine labeled tumor cells I. Studies of MCa‑11 cells grown as tumors in vivo and exponential cultures in vitro.  Cell Tissue Kinet 20:273-90, 1987.

Bose K, Allison, DC. Cytophotometric determination of unscheduled DNA synthesis.  Cytometry 8:203, 1987.

Brooks AL, Jordan SW, Bose K, Smith J, Allison DC.  The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells.  Cell Biology and Toxicology 4:31-40, 1988.

Allison DC, Mayall BH, Levin F, Levin J.  Comparison of Feulgen‑DNA measurements by image analysis and absorption cytometry.  Cytometry, 9:573-8, 1988.

Allison DC, Bose KB, Anderson S, Curley S, Robertson J.  Slowing of cell cycle traverse for cells in exponential monolayer cultures placed into plateau‑fed and starved medium.  Cancer Research 49:1456-64, 1989.

Allison DC.  Alterations in DNA metabolism with tumor growth. In:  Essays in Surgery. Edited by Zarins CK, Churchill Livingstone Inc, NY, 1989, pp 55-64.

Dooley WC, Roberts JR, Allison, DC.  Acid giemsa for rapid identification of mitotic cells.  J. Histochem Cytochem 37:1553-6, 1989.

Bose K, Curley S, Smith W, Allison DC.  Differences in the DNA distributions obtained from  flow and absorption cytometric measurements of mouse hepatocytes and tumor cells.  Cytometry 10:388-93, 1989.

Roberts JR, Rowinski E, Donehower R, Robertson J, Allison DC.  Demonstration of the cell cycle positions for taxol‑induced "asters" and "bundles" by measurement of fluorescence, Feulgen‑DNA content, and autoradiographic labeling of the same cells.  J Histochem Cytochem 37:1659-65, 1989.

Roberts JR, Allison DC, Rowinski E, Donehower R.  Development of polyploidization in taxol resistant human leukemia cells in vitro.  Cancer Research 50:710-6, 1990. 

Dooley WC, Allison DC, Robertson J. Discrepancies between the metaphase, telophase, and the G0/G1 and G2 DNA peaks of heteroploid cell lines.  Cytometry 12:99-106, 1991.

Allison DC, Bose KK, Hruban RH, Piantadosi S, Dooley WC, Boitnott JK, Cameron JL.  Pancreatic cancer cell DNA content correlates with long-term survival after pancreatoduodenectomy. Annals of Surgery 214:648-56, 1991.

Dooley WC, Allison DC.  Non-random distributions of abnormal mitoses in tumor cells.  Cytometry 13:462-8, 1992.

Lin P, Lipnick S, Allison DC.  Evidence that tumor cells with nonmodal DNA content are capable of continued proliferation.  Surgical Forum 43:497-8, 1992.

Bose KK, Allison DC, Hruban RH, Piantadosi S, Zahurak M, Dooley WC, Lin P, Cameron JL.  A comparison of flow-and absorption-cytometric DNA values as prognostic indicators for pancreatic carcinoma.  Cancer 71:691-700, 1993.

Hruban RH, van Mansfeld ADM, Offerhaus GJA, van Weering DHJ, Allison DC, Goodman SN, Kensler TW, Bose KK, Cameron JL, Bos JL.  K-ras oncogene activation in adenocarcinoma of the human pancreas: a study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele specific oligonucleotide hybridization.  Am J Path 143:545-54,1993.

Dooley WC, Allison DC, Lin P, Paul M.  Evidence for altered cell cycle traverse of nonmodal cells of the heteroploid MCa-11 line.  Cell Prolif 26:349-60, 1993.

Lin P, Allison DC.  Measurement of the DNA content and the tritiated-thymidine and bromodeoxyuridine incorporation of the same cells.  J Histochem Cytochem 41:1435-9, 1993.

Lin P, Allison DC, Wainstock J. Durham K, Dooley W, Friedman N, Baker RR.  Impact of  axillary lymph node dissection on the therapy of breast cancer patients.  J Clin Oncol 11:1536-44, 1993.

DiGiuseppe JA, Hruban RH, Goodman SN, Polak M, vanden Berg FM, Allison DC, Cameron SC, Offerhaus JA.  Overexpression of p53 protein in adenocarcinoma of the pancreas.  Amer J Clin Path 101:684-8, 1994.

Bose K, Zahurak, M, Allison, DC.  Differential effects of nitrogen mustard in vivo on the cancer and host cells in Mca-11 tumors.  Cell Proliferation, 28:403-414, 1995.

Klein P, Allison DC, Khuder S, Walsh A, Khan Z, Smith D, Webb T.  Long-term benefits of aggressive treatment for primary colorectal cancer.  J Surg Oncol 62:258-266, 1996.

Smith-Mensah W, Allison DC.  Other prognostic factors for pancreatic cancer.  In: Surgical Diseases of the Pancreas, 3rd Edition.  Edited by Howard JH, Idezuki Y, Ihse, I, Prinz, R.  Williams & Wilkins, Media, PA. pp 491-96, 1997.

Allison DC, Piantodosi S, Hruban RH, Dooley WC, Fishman EK, Yeo CJ, Lillemoe KD, Pitt HA, Lin P, Cameron JL.  DNA content and other factors associated with ten-year survival after resection of pancreatic carcinoma.  J Surg Oncol 67:151-9, 1998. 

Assadnia S, Rapp JP, Nestor AL, Cerilli GJ, Webb TH, Kligman M, Allison DC.  Strain differences in neointimal hyperplasia in the rat.  Circulation Research 84;1252-7, 1999.  Allison DC, Nestor A.  Evidence for a relatively random array of human chromosomes on the mitotic ring.  J Cell Biology 145:1-14, 1999.

Nestor, AL, Allison DC.  Tissue Culture, Cell Growth, and Analysis. In Surgical Research.  Edited by: Souba W, and Wilmore D.  Academic Press, CA, 2001, pp 193-206.

Isaka T, Nestor AL, Takada T, Allison DC.  Chromosomal variations within aneuploid cancer lines.  J Histochem Cytochem 51:1343-53, 2003.

Allison DC, Nestor AL, Isaka T.  Chromosomes During Cell Division. In: Encyclopedia of the Human Genome. Edited by:  Nature Publishing Group, London, UK (2003).

Senchenkov A, Kriegel A. Staren ED, Allison DC.  Use of intraoperative ultrasound in excision of multiple schwannomas of the thigh.  J Clin US 33:360-3, 2005

Nestor, AL, Cicila, GT, Karol, SE, Langenderfer, K, Hollopeter, SL, Allison DC.  Linkage analysis of neointimal hyperplasia and vascular wall transformation following balloon angioplasty.  Physiol Genomics. 25(2):286-93, 2006.

Ways JA, Smith BM, Barbato JC, Ramdath RS, Pettee KM, DeRaedt SJ, Allison DC, Koch, LG, Lee SJ, Cicila GT.  Congenic strains confirm aerobic running capacity quantitative trait loci on rat chromosome 16 and identify possible intermediate phenotypes.  Physiol Genomics.  Dec 19, 2006; [Epub ahead of print].

Smith BM, Zyromski NJ, Allison DC.  Community-Acquired Methicillin-Resistant Staphylococcus Aureus Liver Abscess Requiring Resection.  Surgery 141(1):110-1, 2007.

Nestor AL, Hollopeter SL, Matsui SI, Allison DC.  A Model for Genetic Complementation Controlling the Chromosomal Abnormalities and LOH Formation in Cancer.  Cytogenetic Genome Res. 116(4):235-47, 2007.



Last Updated: 6/9/16