Lake Erie Center

Sampling Efforts

Using iodine and isopropyl alcohol to disinfect the surgical incision site on a
walleye (Sander vitreus)
Harvesting the spleen and kidney using sterile dissection tools
Ph.D. student Lindsey Pierce (Great Lakes Genetics Laboratory) and Castalia State Fish Hatchery Supervisor Dave Insley (Ohio
Division of Wildlife
) processing samples.
In December 2010, a massive gizzard shad die-off was reported at Mac Ray Bay Harbor in Harrisonburg, Michigan. The fish exhibited external and internal hemorrhages associated with the VHS fish virus. Ph.D. student Lindsey Pierce and laboratory technician Vrushalee Palsule collected 20 live gizzard shad for testing with the newly developed StaRT-PCR (Standardized Reverse Transcriptase Polymerase Chain Reaction) assay. Within two weeks, our laboratory team reported the fish as VHSv negative to Michigan DNR officers. The condition of the fish was later confirmed as VHSv free from a certified diagnostic facility.
Ph.D. student Lindsey Pierce (GLGL) and laboratory manager Erin Crawford (George Isaac Cancer Research Center) running fish samples on the Agilent Bioanalyzer 2100 to detect for VHSv.
Last Updated: 6/26/15