Department of Pharmacology and Experimental Therapeutics

Amit K. Tiwari, Ph.D.

Photo of Dr. Tiwari 

Assistant Professor
Department of Pharmacology and Experimental Therapeutics

Phone: 419.383.1913
Frederic and Mary Wolfe Center 282F
amit.tiwari@utoledo.edu
Education
2011-2013 Postdoctoral Research Associate, Mount Sinai School of Medicine, NY
2008-2011 Doctor of Philosophy in Pharmaceutical Sciences, Pharmacology, St. John's University, NY
2006-2008 Master of Science in Pharmaceutical Sciences, Pharmacology, St. John's University, NY
2001-2005 Bachelor of Pharmacy, Pharmaceutical Sciences, Ram-Eesh Institute, U.P.T.U. University

Research Interests

Dr. Tiwari’s Cancer Pharmacology and Systems Therapeutics lab is focused to not only finding a cure for cancer but also on bridging Cancer Health Disparity among segments of the population. Currently, his lab’s efforts are directed in the following key areas:

  • Cancer Chemotherapy - Pharmacokinetics/Pharmacodynamics
  • Multidrug Resistance in Cancer
  • Targeting Factors Associated with Cancer Health Disparity
  • Drug Discovery & Experimental Therapeutics

Publications

Research and Review Articles

  1. Malla R, Wang Y, Chan W, Tiwari AK, Faridi JS. Genetic ablation of PRAS40 improves glucose homeostasis via linking the AKT and mTOR pathways. Biochem Pharmacol. 2015 Apr 27. pii: S0006-2952(15)00239-7. doi: 10.1016/j.bcp.2015.04.016. [Epub ahead of print]
  2. Hassan H, Min BJ, Tiwari AK, Reddy GP, Hinton A, Adesiyun A and Abdela W. Antibacterial activity of plant extracts against food-borne pathogens and spoilage bacteria. International Journal of Poultry Science 14 (x): xx-xx, 2015
  3. Malla R, Ashby CR, Narayanan N, Narayanan B, Faridi JS, and Tiwari AK. Proline-rich AKT substrate of 40-kDa (PRAS40) in the pathophysiology of cancer: Biochemical Biophysical Research Communications, May 7, 2015, In press
  4. Pondugula SR, Ferniany G, Ashraf F, Abbott KL, Smith BF, Coleman ES, Mansour M, Bird RC, Smith AN, Karthikeyan C, Trivedi P, Tiwari AK.Stearidonic acid, a plant-based dietary fatty acid, enhances the chemosensitivity of canine lymphoid tumor cells Biochem Biophys Res Commun. 2015 Apr 4. pii: S0006-291X(15)00607-5. doi: 10.1016/j.bbrc.2015.03.141.
  5. Balaji NV, Ramani MV, Viana AG, Sanglard LP, White J, Mulabagal V, Lee C, Gana TJ, Egiebor NO, Subbaraju GV and Tiwari AK#. Design, Synthesis and In Vitro Cell-based Evaluation of the Anti-cancer Activities of Curcumin and Hispolon Analogs: (Accepted, in press, 2015, Bioorganic Medicinal Chemistry)
  6. Narayanan NK, Tiwari AK, Kunimasa K, Ashby CR, Narayanan B. Potential Role of Natural Dietary Compounds in the Modulation of Metabolomic Fingerprints of Cancer. Accepted, in press Dec, 2014, Metabolomics 5: e131. doi:10.4172/2153-0769.1000e131
  7. Chandrabose K, Lee C, Moore J, Mittal R, Suswam EA, Abott KL, Pondugula SR, Manne U, Trivedi P and Tiwari AK#. Novel Pyrimido[1 ,2 :1,5]-pyrazolo[3,4-b]quinolines derivative circumvents multi drug resistance and produces potent apoptosis in colon cancer cells. (Accepted, in press Nov, 2014, Bioorganic Medicinal Chemistry)
  8. Mansour AM, Abbott KL, Flannery PC, Coleman ES, Tiwari AK, Pondugula SR. Diindolylmethane Inhibits the Activity of P-glycoprotein in 17-71 Canine B-Cell Lymphoid Tumor Cells (Accepted, in press Nov, 2014, The Auburn Univ J of Undergrad Scholarship)
  9. Sodani K, Tiwari AK, Dai, CL, Abuznait AH, Xiao ZJ, Patel A, Ashby C, Kaddoumi A and Chen ZS. Sildenafil enhances the anticancer activity of paclitaxel in an ABCB1- mediated multidrug resistance xenograft mouse model. Journal of Cancer Research Upd, 2014, 3, 169-173
  10. Guo H, Wang DS, Rizwani GH, Ahmed M, Ahmed M, Hassan A, Xu RH, Mansoor N, Tiwari AK and Chen ZS. Antineoplastic activity of Holoptelea integrifolia (Roxb.) Planch bark extracts (in vitro). Pak. J. Pharm. Sci., Vol.26, No.6, November 2013, pp.1151-1156
  11. Tiwari AK# and Koduru SV. Translating Mechanistic Knowledge of Diseases in Clinical Treatment. Trans Clin Bio, 2013; Volume 1 (Issue 1): Pages 1-2 
  12. Mittal R, Agrawal A, Roach R, Buckley T and Tiwari AK#. Acquired Legionnaire’s Disease through Aerosolization of Water from Natural Sources. Trans Clin Bio 2013; Volume 1 (Issue 1): Pages 6-9.
  13. Williams A, Crawford L, Tiwari AK, Samuel T, Wirtu G. Lipid Metabolism in Mammary Neoplasia and Potential Therapeutic Targets. VRI Cell Signaling 2013; Volume 1 (Issue 2): Pages 16-21
  14. Singh S, Makwana DK, Buckley T, Agrawal A, Koduru SV and Tiwari AK#. Clinical Significance of PARP-1 Inhibitors in Cancer Chemotherapy. Trans Clin Bio 2013; Volume 1 (Issue 1): Pages 10-15.
  15. Chen JJ, Sodani K, Xiao ZJ, Tiwari AK, Patel A, Yang DH, Chen SD and Chen ZS. BBA, a synthetic derivative of 23-hydroxybutulinic acid, reverses multidrug resistance by inhibiting the efflux activity of MRP7 (ABCC10). PLoS One. 2013 Sep 17;8(9):e74573. doi: 10.1371/journal.pone.0074573.
  16. Tiwari AK. Cell signaling to systems biology. Editorial, July 1st, 2013 p.1-2, Vol.1, VRI Cell signaling
  17. Tiwari AK#, and Chen ZS. Repurposing PDE5 inhibitors as a chemoadjuvants. (June 25th, 2013, Frontiers in Pharmacology)
  18. Chitranshi N, Tiwari AK, and Tripathi PK. Investigating the function of single nucleotide polymorphism (SNPs) in CTSB gene (cathepsin B): a computational approach. (Accepted May 18th, Future Neurology)
  19. Patel A, Tiwari AK, Chufan EE, Sodani K, Anreddy N, Singh S, Ambudkar SV, Stephani R and Chen ZS. PD17384, human VEGFR inhibitor, reverses ABCB1 mediated drug resistance in cancer cells. (May 15th, 2013, Cancer Chemotherapy and Pharmacology) PMID: 23673445 (Cited 9 times: as of 09-01-14)
  20. Tiwari AK, Zhang R and Gallo JM. Overlapping functions of ABC transporters in topotecan disposition as determined in gene knockout mouse models. (online first, May 1, 2013 Mol Cancer Ther) PMID: 23635651 (Cited 3 times: as of 09-01-14)
  21. Tiwari AK, Sodani K, Dai CL, Abuznait AH, Singh S, Xiao ZJ, Vispute S, Patel P, Talele TT, Fu LW, Kaddoumi A, Gallo JM, Chen ZS. Nilotinib potentiates anticancer drug sensitivity in murine ABCB1-, ABCG2-, and ABCC10-multidrug resistance xenograft models. Cancer Lett. 2012 Oct 9. [Epub ahead of print] PMID: 23063650 (Cited 20 times: as of 09-01-14)
  22. Kuang YH, Patel J, Wu CP, Sodani K, Dai CL, Tiwari AK, Lee J, Fu LW, Ambudkar SV, Korlipara V, Chen ZS. OSI-930 analogues as novel reversal agents for ABCG2-mediated multidrug resistance. Biochem Pharmacol, 2012 Sep 15;84(6):766-74. PMID: 22750060 (Cited 6 times: as of 09-01-14)
  23. Chen JJ, Sun YL, Tiwari AK, Xiao ZJ, Sodani K, Vispute SG, Yang DH, Chen SD and Chen ZS. PDE5 inhibitors, sildenafil and vardenafil, reverse multidrug resistance by inhibiting the efflux function of MRP7 (ABCC10) transporter. Cancer Sci. 2012 May 11. doi: 10.1111/j.1349-7006.2012.02328.x. [Epub ahead of print] PMID: 22578167 (Cited 10 times: as of 09-01-14)
  24. Li T, Yang Y, Cheng C, Tiwari AK, Sodani K, Zhao Y, Chen ZS, . N-biphenyl (terphenyl)- 2,2-dichloroacet-amide analogues as anti-cancer drugs: design, synthesis and biological evaluation. Bioorg Med Chem Lett. 2012 Jul 23. ii: S0960-894X(12)00933-X. doi: 10.1016/j.bmcl. 2012.07.057. PMID: 23067553 (Cited 3 times: as of 09-01-14)
  25. Sodani K, Tiwari AK, Dai CL, Patel A, Chen ZS. GW583340 and GW2974, Human EGFR and HER-2 inhibitors, reverse ABCB1- and ABCG2-mediated drug resistance. Biochem Pharmacol. 2012 Jun 15;83(12):1613-22. Epub 2012 Mar 7. PMID: 22414725 (Cited 15 times: as of 09-01-14)
  26. Peng XX, Tiwari AK, Wu HC and Chen ZS. Overexpression of P-glycoprotein induces acquired resistance to imatinib in chronic myeloid leukemia cells. Chin J Cancer. 2012 Feb;31(2):110-8. Epub 2011 Nov 18. PMID: 22098951 (Cited 5 times: as of 09-01-14)
  27. Chen ZS and Tiwari AK. Multidrug resistance proteins (MRPs/ABCCs) in cancer chemotherapy and human genetic diseases. FEBS J. 2011 Sep;278(18):3226-45. Epub 2011 Aug 1. PMID: 21740521 (Cited 86 times: as of 09-01-14) (Note: Top citation award 2011- 2013)
  28. Shi Z, Tiwari AK, Patel A, Fu LW. Chen ZS. Roles of sildenafil in enhancing drug sensitivity in cancer. Cancer Res, 2011 Jun 1;71(11):3735-8. Epub 2011 May 24. PMID: 21610107 (Cited 16 times: as of 09-01-14)
  29. Ding PR, Tiwari AK, Ohnuma S, Lee JWKK, An X, Dai CL, Lu Q, Singh S, Talele TT, Ambudkar SV, Chen ZS. The phosphodiesterase-5 inhibitor vardenafil is a potent inhibitor of ABCB1/P-glycoprotein. Submitted to Plos One, 2011 Apr 28;6(4):e19329. PMID: 21552528 (Cited 35 times: as of 09-01-14)
  30. Shi Z, Tiwari AK, Shukla S, Robey RW, Singh S, Kim IW, Bates SE, Peng XX, Abraham I, Ambudkar SV, Talele TT, Fu LW, Chen ZS. Sildenafil reverses ABCB1- and ABCG2- mediated chemotherapeutic drug resistance. Cancer Res, 2011 Apr 15;71(8):3029-41. Epub 2011 Mar 14. PMID: 21402712 (Cited 43 times: as of 09-01-14)
  31. Peng XX, Shi Z, Tiwari AK, Damaraju VL, Fu L, Cass CE, Kruh GD and Chen ZS. Upregulation of P-gp in human chronic myeloid leukemia cells with acquired resistance to 6-mercaptopurine. Oncol Lett, 2011 May-Jun 2 (3):549-56.
  32. Tiwari AK, Sodani K, Dai CL, Ashby C, Chen ZS. Revisiting the ABCs of Multidrug Resistance in Cancer Chemotherapy. Curr Pharm Biotechnol. 2011 Apr 1;12(4):570-94. PMID: 21118094 (Cited 68 times: as of 09-01-14) (Note: Top 5 most cited article in Curr Pharm Biotechnol)
  33. Mi YJ, Liang YJ, Huang HB, Zhao HY, Wu CP, Wang F, Tao LY, Zhang CZ, Dai CL, Tiwari AK, Ma XX, To KKW, Ambudkar SV, Chen ZS, and Fu LW. Apatinib (YN968D1) Reverses Multidrug Resistance by Inhibiting the Efflux Function of Multiple ATPBinding Cassette Transporters. Cancer Res 2010 Oct 15;70(20):7981-91. Epub 2010 Sep 28. PMID: 20876799 (Cited 68 times: as of 09-01-14)
  34. Tiwari AK, Xin A, Chen ZS. The role of stem cell markers in multidrug resistance mediated by ABC transporters. Leukemia Res 34, 696–697, 2010 doi:10.1016/j.leukres.2009.12.007. PMID: 20061021 (Cited 4 times: as of 09-01-14)
  35. An X*, Tiwari AK*, Sun Y, Ding PR, Ashby CR, and Chen ZS. BCR-ABL tyrosine kinase inhibitors in the treatment of philadelphia chromosome positive chronic myeloid leukemia: a review. Leuk Res. 2010 Oct;34(10):1255-68. PMID: 20537386 (*Joint-First Author) (Cited 77 times: as of 09-01-14) (Note: Top 10 most cited article in leukemia research)
  36. Kuang YH, Shen T, Chen X, Sodani K, Hopper-Borge E, Tiwari AK, Lee JW, Fu LW and Chen ZS Lapatinib and erlotinib are potent reversal agents for MRP7 (ABCC10)-mediated multidrug resistance. Biochem Pharmacol 2010 Jan 15;79(2):154-61. Epub 2009 Aug 29. PMID: 19720054 (Cited 51 times: as of 09-01-14)
  37. Shen T, Kuang YH, Ashby CR, Lei Y, Chen A, Zhou Y, Chen X, Tiwari AK, Hopper-Borge E, Ouyang J and Chen ZS. Imatinib and nilotinib reverse multidrug resistance in cancer cells by inhibiting the efflux activity of the MRP7 (ABCC10). PLoS One 2009 Oct 20;4(10):e7520. PMID: 19841739 (Cited 39 times: as of 09-01-14)
  38. Tiwari AK#, Sodani K, Chen ZS. Current advances in modulation of ABC transportermediated multidrug resistance in cancer. Invited review. International J of Pharml and Clin Res, 2009. 1(1): 1-6 (Cited 3 times: as of 09-01-14) (*Correspondence)
  39. Tiwari AK, Sodani K, Wang SR, Kuang YH, Ashby CR, Jr., Chen X and Chen ZS. Nilotinib (AMN107, Tasigna) reverses multidrug resistance by inhibiting the activity of the ABCB1/Pgp and ABCG2/BCRP/MXR transporters. Biochem Pharmacol 2009 Jul 15;78(2):153-61. Epub 2009 Apr 11. PMID: 19427995 (Cited 107 times: as of 09-01-14) (*Note: Top citation award from Elsevier for top cited article in biochemical pharmacology from 2009-2011) ** Also, 4th and 5th most hottest article at Sciencedirect.com from jun-dec 2009 http://top25.sciencedirect.com/subject/pharmacologytoxicology-and-pharmaceutical-science/20/journal/biochemicalpharmacology/00062952/archive/22/
  40. Dai CL, Liang YJ, Wang YS, Tiwari AK, Yan YY, Wang F, Chen ZS, Tong XZ and Fu LW. Sensitization of ABCG2-overexpressing cells to conventional chemotherapeutic agent by sunitinib was associated with inhibiting the function of ABCG2. Cancer Lett 2009 Jun 28;279(1):74-83. Epub 2009 Feb 18. PMID: 19232821 (Cited 60 times: as of 09-01-14)
  41. Shi Z, Tiwari AK, Shukla S, Robey RW, Kim IW, Parmar S, Bates SE, Si QS, Goldblatt CS, Abraham I, Fu LW, Ambudkar SV and Chen ZS. Inhibiting the function of ABCB1 and ABCG2 by the EGFR tyrosine kinase inhibitor AG1478. Biochem Pharmacol 2009 Mar 1;77(5):781-93. Epub 2008 Nov 18. PMID: 19059384 (Cited 32 times: as of 09-01-14)
  42. Dai CL*, Tiwari AK*, Wu CP, Su XD, Wang SR, Liu DG, Ashby CR, Jr., Huang Y, Robey RW, Liang YJ, Chen LM, Shi CJ, Ambudkar SV, Chen ZS and Fu LW. Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2. Cancer Res 2008 Oct 1;68(19):7905-14 PMID: 18829547 (*Joint-First Author). (Cited 156 times: as of 09-01-14)
  43. Peng XX, Shi Z, Damaraju VL, Huang XC, Kruh GD, Wu HC, Zhou Y, Tiwari AK, Fu L, Cass CE and Chen ZS. Up-regulation of MRP4 and down-regulation of influx transporters in human leukemic cells with acquired resistance to 6-mercaptopurine. Leuk Res 32:799-809, 2008. PMID: 17996297 (Cited 34 times: as of 09-01-14)

Book Chapters

  1. Shi Z*, Tiwari AK*, Ashby CR, Fu LW and Chen ZS. Anticancer role of Phospodiesterase 5 inhibitors. The Research and Biology of Cancer I. ISBN: 978-1-477554-999. (*Joint-First Author).
  2. Tiwari AK#, Yang DH, and Chen ZS. Molecular mechanisms of methotrexate resistance. Nova Science Publishers, Inc. ISBN – 978-1-62100-596-4, 2012
  3. Mittal R, Agrawal A, Tourne M, Koduru SV, Viana AG, Reddy G, and Tiwari AK#. Nanoparticle-Based Therapeutics to Circumvent Cancer Chemoresistance. 2013. Recent Developments in Biotechnology. Studium Press LLC, USA (in press), 2014.
Last Updated: 6/9/16